A5042250612- Metabolomics and Mass Spectrometry Studies
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Photosynthetic Processes and Mechanisms
- Immune cells in cancer
- Biochemical effects in animals
- Stress Responses and Cortisol
- Plant nutrient uptake and metabolism
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Immune Cell Function and Interaction
- Aldose Reductase and Taurine
- Polyamine Metabolism and Applications
- Free Radicals and Antioxidants
- Acute Myeloid Leukemia Research
Heidelberg University
2016-2025
Heidelberg University
2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2011
University of Kaiserslautern
2011
Aryl hydrocarbon receptor (AHR) activation by tryptophan (Trp) catabolites enhances tumor malignancy and suppresses anti-tumor immunity. The context specificity of AHR target genes has so far impeded systematic investigation activity its upstream enzymes across human cancers. A pan-tissue signature, derived natural language processing, revealed that 32 entities, interleukin-4-induced-1 (IL4I1) associates more frequently with than IDO1 or TDO2, hitherto recognized as the main Trp-catabolic...
Abstract The dynamics and phenotypes of intratumoral myeloid cells during tumor progression are poorly understood. Here we define cellular states in gliomas by longitudinal single-cell profiling demonstrate their strict control the genotype: isocitrate dehydrogenase (IDH)-mutant tumors, differentiation infiltrating is blocked, resulting an immature phenotype. In late-stage gliomas, monocyte-derived macrophages drive tolerogenic alignment microenvironment, thus preventing T cell response. We...
Abstract Aberrant expression of MYC transcription factor family members predicts poor clinical outcome in many human cancers. Oncogenic profoundly alters metabolism and mediates an antioxidant response to maintain redox balance. Here we show that MYCN induces massive lipid peroxidation on depletion cysteine, the rate-limiting amino acid for glutathione (GSH) biosynthesis, sensitizes cells ferroptosis, oxidative, non-apoptotic iron-dependent type cell death. The high cysteine demand...
Abstract Postnatal maturation of cardiomyocytes is characterized by a metabolic switch from glycolysis to fatty acid oxidation, chromatin reconfiguration and exit the cell cycle, instating barrier for adult heart regeneration 1,2 . Here, explore whether reprogramming can overcome this enable regeneration, we abrogate oxidation in inactivation Cpt1b We find that disablement improves resistance hypoxia stimulates cardiomyocyte proliferation, allowing after ischaemia–reperfusion injury....
Summary The vacuolar membrane is involved in solute uptake into and release from the vacuole, which largest plant organelle. In addition to inorganic ions metabolites, large quantities of protons sugars are shuttled across this membrane. Current models suggest that proton gradient drives accumulation and/or sugars. Recent studies have associated AtSUC4 with Some members SUC family plasma proton/sucrose symporters. addition, sugar transporters TMT1 TMT2, localized membrane, been suggested...
Abstract Growth of eukaryotic cells is regulated by the target rapamycin (TOR). The strongest activator TOR in metazoa amino acid availability. established transducers sensing to are absent plants. Hence, a fundamental question how achieved photo-autotrophic organisms. Here we demonstrate that plant Arabidopsis does not sense sulfur-containing cysteine itself, but its biosynthetic precursors. We identify kinase GCN2 as sensor carbon/nitrogen precursor availability, whereas limitation sulfur...
Chemotaxis enables bacteria to navigate chemical gradients in their environment, accumulating toward high concentrations of attractants and avoiding repellents. Although finding nutrients is likely be an important function bacterial chemotaxis, not all characterized are nutrients. Moreover, even for potential nutrients, the exact relation between metabolic value chemicals efficiency as chemoattractants has been systematically explored. Here we compare chemotactic response amino acids with...
Plant stem cell niches, the meristems, require long-distance transport of energy metabolites and signaling molecules along phloem tissue. However, currently it is unclear how specification cells controlled. Here we show that genes SUPPRESSOR OF MAX2 1-LIKE3 (SMXL3), SMXL4, SMXL5 act as cell-autonomous key regulators formation in Arabidopsis thaliana. The three form an uncharacterized subclade SMXL gene family mediates hormonal strigolactone karrikin signaling. Strigolactones are endogenous...
Background: Nutrients are transported through endothelial cells before being metabolized in muscle cells. However, little is known about the regulation of transport processes. Notch signaling a critical regulator metabolism and angiogenesis during development. Here, we studied how genetic pharmacological manipulation adult mice affects fatty acid transport, cardiac angiogenesis, heart function. Methods: Endothelial-specific inhibition was achieved by conditional inactivation Rbp-jκ to...
Abstract Subcellular sugar partitioning in plants is strongly regulated response to developmental cues and changes external conditions. Besides transitory starch, the vacuolar sugars represent a highly dynamic pool of instantly accessible metabolites that serve as energy source osmoprotectant. Here, we present molecular identification functional characterization glucose (Glc) exporter Arabidopsis (Arabidopsis thaliana) Early Responsive Dehydration-Like6 (AtERDL6). We demonstrate tonoplast...
Seeds preserve a far developed plant embryo in quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since switchover from quiescence reactivation provides remarkable case of cell physiological transition we investigated earliest events energy redox Arabidopsis seeds at imbibition. By developing fluorescent protein biosensing intact seeds, observed ATP accumulation oxygen uptake within minutes,...
FOXP1 syndrome caused by haploinsufficiency of the forkhead box protein P1 (FOXP1) gene is a neurodevelopmental disorder that manifests motor dysfunction, intellectual disability, autism, and language impairment. In this study, we used Foxp1+/- mouse model to address whether cognitive deficits in are associated with mitochondrial dysfunction oxidative stress. Here, show genes role biogenesis dynamics (e.g., Foxo1, Pgc-1α, Tfam, Opa1, Drp1) were dysregulated striatum mice at different...
Glioblastoma is a deadly brain tumor for which there no cure. The presence of glioblastoma stem-like cells (GSCs) contributes to the heterogeneous nature disease and makes developing effective therapies challenging. have been shown influence their environment by releasing biological nanostructures known as extracellular vesicles (EVs). Here, we investigated role GSC-derived nanosized EVs (<200 nm) in heterogeneity, plasticity, aggressiveness, with particular focus on protein, metabolite,...
CD4 + regulatory T (T reg ) cells accumulate in the tumor microenvironment (TME) and suppress immune system. Whether how metabolite availability TME influences cell differentiation is not understood. Here, we measured 630 metabolites found that serine palmitic acid, substrates required for synthesis of sphingolipids, were enriched. A serine-free diet or a deficiency Sptlc2, rate-limiting enzyme catalyzing sphingolipid synthesis, suppressed accumulation inhibited growth. Sphinganine, an...
Cell metabolism reprogramming to sustain energy production, while reducing oxygen and consuming processes is crucially important for the adaptation hypoxia/ischemia. Adaptive metabolic rewiring controlled by hypoxia-inducible factors (HIFs). Accumulating experimental evidence indicates that timely activation of HIF in brain-resident cells improves outcome from acute ischemic stroke. However, underlying molecular mechanisms are still incompletely understood. Thus, we investigated whether...
Abstract Microglia, brain-resident macrophages, can acquire distinct functional phenotypes, which are supported by differential reprogramming of cell metabolism. These adaptations include remodeling in glycolytic and mitochondrial metabolic fluxes, potentially altering energy substrate availability at the tissue level. This phenomenon may be highly relevant brain, where metabolism must precisely regulated to maintain appropriate neuronal excitability synaptic transmission. Direct evidence...
Summary Arabidopsis vacuoles harbor, besides sugar transporter of the TMT ‐type, an early response to dehydration like 6 ( ERDL 6) protein involved in glucose export into cytosol. However, mode transport and plant's feedback overexpression its activity on essential properties such as, for example, seed germination or freezing tolerance, remain unexplored. Using patch‐clamp studies expressing At we demonstrated directly that this carrier operates as a proton‐driven exporter. Overexpression Bv...
Plant and inflorescence architecture determine the yield potential of crops. Breeders have harnessed natural diversity for to improve yields, induced genetic variation could provide further gains. Wheat is a vital source protein calories; however, little known about genes that regulate development its inflorescence. Here, we report identification semidominant alleles class III homeodomain-leucine zipper transcription factor, HOMEOBOX DOMAIN-2 (HB-2), on wheat A D subgenomes, which generate...