A5016402885- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Dietary Effects on Health
- Immune Response and Inflammation
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Immune cells in cancer
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Tryptophan and brain disorders
- Genetics, Aging, and Longevity in Model Organisms
- Diet and metabolism studies
- Lipid metabolism and biosynthesis
- Sirtuins and Resveratrol in Medicine
- Cancer, Hypoxia, and Metabolism
- Nitric Oxide and Endothelin Effects
- Advanced Fluorescence Microscopy Techniques
- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Alcohol Consumption and Health Effects
- Neurological Disease Mechanisms and Treatments
- Peroxisome Proliferator-Activated Receptors
- Stress Responses and Cortisol
- Barrier Structure and Function Studies
Heidelberg University
2019-2024
Universidade Estadual de Campinas (UNICAMP)
2017-2020
Universidade de São Paulo
2011-2019
Instituto de Botânica
2012
Type II IFN (IFN-γ) is a proinflammatory T lymphocyte cytokine that serves in priming of microglia-resident CNS macrophages-during the complex microglial activation process under pathological conditions. Priming generally permits an exaggerated response to secondary inflammatory stimulus. The impact primed microglia on physiological neuronal function intact cortical tissue (in situ) widely unknown, however. We explored effects chronic IFN-γ exposure hippocampal slice cultures, i.e.,...
Caloric restriction (CR) protects against many cerebral pathological conditions that are associated with excitotoxic damage and calcium overload, although the mechanisms still poorly understood. Here we show CR strongly insults in vitro vivo a manner significant changes mitochondrial function. increases electron transport chain activity, enhances antioxidant defenses, favors retention capacity brain. These accompanied by decrease cyclophilin D activity acetylation an increase Sirt3...
Abstract Microglia, brain-resident macrophages, can acquire distinct functional phenotypes, which are supported by differential reprogramming of cell metabolism. These adaptations include remodeling in glycolytic and mitochondrial metabolic fluxes, potentially altering energy substrate availability at the tissue level. This phenomenon may be highly relevant brain, where metabolism must precisely regulated to maintain appropriate neuronal excitability synaptic transmission. Direct evidence...
Intermittent fasting (IF) is a dietary intervention often used as an alternative to caloric restriction (CR) and characterized by 24 hour cycles alternating ad libitum feeding fasting. Although the consequences of CR are well studied, effects IF on redox status not. Here, we address state markers in different tissues order uncover how changes frequency alter balance rats. rats displayed lower body mass due decreased energy conversion efficiency. Livers presented increased mitochondrial...
Abstract Microglial activation by oleate and palmitate differentially modulates brain inflammatory status. However, the metabolic reprogramming supporting these reactive phenotypes remains unknown. Employing real-time measurements lipidomic analysis, we show that both fatty acids promote microglial oxidative metabolism, while lipopolysaccharide (LPS) enhances glycolytic rates. Interestingly, treatment was followed enrichment in storage lipids bound to polyunsaturated (PUFA), parallel with...
Abstract Background The granulocyte-macrophage colony-stimulating factor (GM-CSF) (or CSF-2) is involved in myeloid cell growth and differentiation, and, possibly, a major mediator of inflammation body tissues. role GM-CSF the activation microglia (CNS resident macrophages) consequent impacts on neuronal survival, excitability, synaptic transmission are widely unknown, however. Here, we focused electrical network rhythms gamma frequency band (30–70 Hz). Gamma oscillations fundamental to...
Calorie restriction is a dietary intervention known to improve redox state, glucose tolerance, and animal life span. Other interventions have been adopted as study models for caloric restriction, including nonsupplemented food intermittent, every-other-day feedings. We compared the short- long-term effects of these ad libitum protocols found that, although all restricted diets decrease body weight, intermittent feeding did not intra-abdominal adiposity. Short-term calorie presented similar...
Intermittent fasting (IF) is an often-used intervention to decrease body mass. In male Sprague-Dawley rats, 24 hour cycles of IF result in light caloric restriction, reduced mass gain, and significant decreases the efficiency energy conversion. Here, we study metabolic effects order uncover mechanisms involved this lower conversion efficiency. After 3 weeks, animals displayed overeating during fed periods mass, accompanied by alterations energy-related tissue The use was not due uncoupling...
β-cells quickly adjust insulin secretion to oscillations in nutrients carried by the blood, acting as fuel sensors. However, most studies of β-cell responses do not discriminate between levels and signaling components present circulation. Here we studied effect serum from calorie-restricted rats versus fed ad libitum, diluted tenfold medium, which did contribute significantly pool nutrients, on mitochondrial function dynamics under regular high-nutrient culture conditions. Insulin secreting...
Breast cancer is a prevalent neoplastic disease among women worldwide which treatments still present several side effects and resistance. Considering that cells derangements in their energetic homeostasis, peroxisome proliferator-activated receptor- gamma coactivator 1 (PGC-1) crucial for cellular metabolism redox signaling, the main objective of this study was to investigate whether there relationship between PGC-1 expression, proliferation breast mechanisms involved. We initially assessed...
Obesity-derived inflammation and metabolic dysfunction has been related to the activity of inducible nitric oxide synthase (iNOS). To understand interrelation between metabolism, obesity NO., we evaluated effects obesity-induced NO. signaling on liver mitochondrial function. We used mouse strains containing nicotinamide transhydrogenase activity, while prior studies involved a spontaneous mutant this enzyme, are, therefore, more prone oxidative imbalance. Wild-type iNOS knockout mice were...
Abstract Microglial cells (resident macrophages) feature rapid activation in CNS disease and can acquire multiple phenotypes exerting neuroprotection or neurotoxicity. The functional impact of surveying (“resting”) microglia on neural excitability neurotransmission physiology is widely unknown, however. We addressed this issue male rat hippocampal slice cultures (in situ) by pharmacological microglial ablation within days characterizing neuronal gamma‐band oscillations (30–70 Hz) that are...