A5056415473- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Biochemical Analysis and Sensing Techniques
- Stress Responses and Cortisol
- Pancreatic function and diabetes
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Dietary Effects on Health
- Birth, Development, and Health
- Eating Disorders and Behaviors
- Alzheimer's disease research and treatments
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Aldose Reductase and Taurine
- Cholesterol and Lipid Metabolism
- Bioactive natural compounds
- Neonatal and fetal brain pathology
- Ginseng Biological Effects and Applications
- Obesity, Physical Activity, Diet
- Antioxidant Activity and Oxidative Stress
- Circadian rhythm and melatonin
Universidade Estadual de Campinas (UNICAMP)
2014-2025
Hospital de Clínicas da Unicamp
2025
Institute of Molecular Biology and Biophysics
2016
In diet-induced obesity, hypothalamic and systemic inflammatory factors trigger intracellular mechanisms that lead to resistance the main adipostatic hormones, leptin insulin. Tumor necrosis factor-α (TNF-α) is one of produced during this process its mechanistic role as an inducer insulin has been widely investigated. Most TNF-α signals are delivered by TNF receptor 1 (R1); however, played in context obesity-associated inflammation not completely known. Here, we show TNFR1 knock-out (TNFR1...
Hypothalamic inflammation is a common feature of experimental obesity. Dietary fats are important triggers this process, inducing the activation toll-like receptor-4 (TLR4) signaling and endoplasmic reticulum stress. Microglia cells, which cellular components innate immune system in brain, expected to play role early diet-induced hypothalamic inflammation. Here, we use bone marrow transplants generate mice chimeras that express functional TLR4 entire body except marrow–derived cells or only...
The consumption of large amounts dietary fats is one the most important environmental factors contributing to development obesity and metabolic disorders. GPR120 GPR40 are polyunsaturated fatty acid receptors that exert a number systemic effects beneficial for inflammatory diseases. Here, we evaluate expression potential role hypothalamic as targets treatment obesity. Male Swiss (6-weeks old), were fed with high fat diet (HFD, 60% kcal from fat) 4 weeks. Next, mice underwent stereotaxic...
The peel of the native Brazilian fruit jaboticaba is rich in anthocyanins, which are known for their anti-obesity effects animal models. aim present study was to evaluate freeze-dried powder (FDJPP) on a number metabolic parameters model diet-induced obesity. Mice ( n 8 per group) were initially fed high-fat diet (HFD, 35 % w/w) 4 weeks and then switched HFD supplemented with FDJPP (1, 2 or an additional 6 weeks. Energy intake, weight loss, glucose tolerance, insulin resistance lipid profile...
Obesity is the result of a long-term positive energy balance in which caloric intake overrides expenditure. This anabolic state results from defective activity hypothalamic neurons involved sensing and response to adiposity. However, it currently unknown what earliest obesity-linked defect how orchestrates imbalance present obesity. Using an outbred model diet-induced obesity we show that regulation POMC marker distinguishing obesity-prone obesity-resistant mice. The early inhibition was...
TNFα is an important mediator of catabolism in cachexia. Most its effects have been characterized peripheral tissues, such as skeletal muscle and fat. However, by acting directly the hypothalamus, can activate thermogenesis modulate food intake. Here we show that high concentration hypothalamus leads to increased O2 consumption/CO2 production, body temperature, reduced caloric intake, resulting loss mass. thermogenic response produced β3-adrenergic signaling brown adipose tissue (BAT),...
Fructose consumption causes insulin resistance and favors hepatic gluconeogenesis through mechanisms that are not completely understood. Recent studies demonstrated the activation of hypothalamic 5′-AMP-activated protein kinase (AMPK) controls dynamic fluctuations in glucose production. Thus, present study was designed to investigate whether AMPK by fructose would mediate increased gluconeogenesis. Both ip intracerebroventricular (icv) treatment stimulated acetyl-CoA carboxylase...
Apoptosis of hypothalamic neurons is believed to play an important role in the development and perpetuation obesity. Similar hippocampus, hypothalamus presents constitutive stimulated neurogenesis, suggesting that obesity-associated dysfunction can be repaired. Here, we explored hypothesis n-3 polyunsaturated fatty acids (PUFAs) induce neurogenesis. Both diet injected directly into hypothalamus, PUFAs were capable increasing neurogenesis levels similar or superior effect brain-derived...
Intermittent fasting (IF) is an often-used intervention to decrease body mass. In male Sprague-Dawley rats, 24 hour cycles of IF result in light caloric restriction, reduced mass gain, and significant decreases the efficiency energy conversion. Here, we study metabolic effects order uncover mechanisms involved this lower conversion efficiency. After 3 weeks, animals displayed overeating during fed periods mass, accompanied by alterations energy-related tissue The use was not due uncoupling...
Fibroblast growth factor 19 (FGF19) signaling in the brain is associated with body weight loss, reduced food intake, and improved glycemic control obese mice through unclear mechanisms. Here, we investigated effects of central FGF19 administration on peripheral tissues, focusing adipose tissue, its contributions to loss. Using single-cell RNA sequencing adult murine hypothalamus, found that has potential target multiple cell populations, including astrocytes-tanycytes, microglia, neurons,...
The consumption of large amounts dietary fats activates an inflammatory response in the hypothalamus, damaging key neurons involved regulation caloric intake and energy expenditure. It is currently unknown why mediobasal hypothalamus main target diet-induced brain inflammation. We hypothesized that can damage median eminence blood/spinal fluid interface. Swiss mice were fed on a high-fat diet, molecular structural studies performed employing real-time PCR, immunoblot, immunofluorescence,...
Obesity is one of the leading noncommunicable diseases in world. Despite intense efforts to develop strategies prevent and treat obesity, its prevalence continues rise worldwide. A recent study has shown that tricarboxylic acid intermediate succinate increases body energy expenditure by promoting brown adipose tissue thermogenesis through activation uncoupling protein-1; this generated interest surrounding potential usefulness as an approach obesity. It currently unknown how impacts protein...
Maternal obesity is an important risk factor for obesity, cardiovascular, and metabolic diseases in the offspring. Studies have shown that it leads to hypothalamic inflammation progeny, affecting function of neurons regulating food intake energy expenditure. In adult mice fed a high-fat diet, one abnormalities contribute development damage blood-brain barrier (BBB) at median eminence-arcuate nucleus (ME-ARC) interface; however, how BBB affected offspring obese mothers requires further...
Free fatty acid receptor-1 (FFAR1) is a medium- and long-chain sensing G protein-coupled receptor that highly expressed in the hypothalamus. Here, we investigated central role of FFAR1 on energy balance. Central agonism virogenic knockdown were performed mice. Energy balance studies, infrared thermographic analysis brown adipose tissue (BAT) molecular hypothalamus, BAT, white (WAT) liver carried out. Pharmacological stimulation FFAR1, using administration its agonist TUG-905 diet-induced...
Hypothalamic adult neurogenesis provides the basis for renewal of neurons involved in regulation whole-body energy status. In addition to hormones, cytokines and growth factors, components diet, particularly fatty acids, have been shown stimulate hypothalamic neurogenesis; however, mechanisms behind this action are unknown. Here, we hypothesized that GPR40 (FFAR1), receptor medium long chain unsaturated could mediate at least part neurogenic activity hypothalamus. We show a ligand increased...
The consumption of large amounts dietary fats can trigger an inflammatory response in the hypothalamus and contribute to dysfunctional control caloric intake energy expenditure commonly present obesity. objective this study was identify chemokine-related transcripts that could be involved early stages diet-induced hypothalamic inflammation. We used immunoblot, PCR array, real-time PCR, immunofluorescence staining, glucose insulin tolerance tests, determination general metabolic parameters...
In experimental obesity, the hypothalamus is affected by an inflammatory response activated dietary saturated fats. This inflammation triggered as early one day after exposure to a high-fat diet, and during its progression, there recruitment of cells from systemic circulation. The objective present study was identifying chemokines potentially involved in development hypothalamic diet-induced inflammation. order identify this process, we performed real-time PCR array that determined Ackr2...