A5048638699- Pancreatic function and diabetes
- Metabolism, Diabetes, and Cancer
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Endoplasmic Reticulum Stress and Disease
- Genetics and Neurodevelopmental Disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Regulation of Appetite and Obesity
- Diet, Metabolism, and Disease
- Birth, Development, and Health
- Drug Transport and Resistance Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Diet and metabolism studies
- Fibroblast Growth Factor Research
- Connexins and lens biology
- Biochemical effects in animals
- Liver Disease Diagnosis and Treatment
- Diabetes Management and Research
- Alzheimer's disease research and treatments
- Epigenetics and DNA Methylation
- Innovative Teaching Methods
- Problem and Project Based Learning
- Cannabis and Cannabinoid Research
- Ubiquitin and proteasome pathways
- Peroxisome Proliferator-Activated Receptors
Universidade Estadual de Campinas (UNICAMP)
2016-2025
Hospital de Clínicas da Unicamp
2025
King's College London
2011-2015
Orthopaedic Center
2004
Progressive reduction in β-cell mass is responsible for the development of type 2 diabetes mellitus, and alteration insulin receptor substrate (IRS-2) abundance plays a critical role this process. IRS-2 expression stimulated by transcription factor cAMP response element-binding protein (CREB) we recently demonstrated that Ca(2+)/calmodulin dependent kinase 4 (CaMK4) upstream CREB activation β-cells. This study investigated whether CaMK4 also potential target to increase through CREB-mediated...
Fibroblast growth factor 19 (FGF19) signaling in the brain is associated with body weight loss, reduced food intake, and improved glycemic control obese mice through unclear mechanisms. Here, we investigated effects of central FGF19 administration on peripheral tissues, focusing adipose tissue, its contributions to loss. Using single-cell RNA sequencing adult murine hypothalamus, found that has potential target multiple cell populations, including astrocytes-tanycytes, microglia, neurons,...
Tumor necrosis factor-alpha (TNF-alpha) is known to participate in the wastage syndrome that accompanies cancer and severe infectious diseases. More recently, a role for TNF-alpha pathogenesis of type 2 diabetes mellitus obesity has been shown. Much regulatory action exerted by upon control energy stores depends on its hypothalamus. In this study, we show activates canonical pro-inflammatory signal transduction pathways hypothalamus rats. These signaling events lead transcriptional...
Susceptibility during fasting has been reported for the common vampire bat (Desmodus rotundus), to point of untimely deaths after only 2-3 nights fasting. To investigate underlying physiology this critical metabolic condition, we analyzed serum insulin levels, pancreatic islets morphometry and immunocytochemistry (ICC), static secretion in pancreas fragments, signaling mechanism male bats. A glucose tolerance test (ipGTT) was also performed. Serum found be lower fed vampires compared other...
In the present study, we investigated relationship between early life protein malnutrition-induced redox imbalance, and reduced glucose-stimulated insulin secretion. After weaning, male Wistar rats were submitted to a normal-protein-diet (17%-protein, NP) or low-protein-diet (6%-protein, LP) for 60 days. Pancreatic islets isolated hydrogen peroxide (H2 O2 ), oxidized (GSSG) (GSH) glutathione content, CuZn-superoxide dismutase (SOD1), peroxidase (GPx1) catalase (CAT) gene expression, as well...
Fetal and neonatal pancreatic islets present a lower insulin secretory response as compared with adult islets. Prolonged culturing leads to an improvement of the glucose-induced secretion in that may involve regulation gap junction mediated cell communication. In this study, we investigated effect islet cells for varying periods time different glucose medium concentrations on cellular expression endocrine associated connexin (Cx) 36 Cx43. We report here 7-d culture induced upregulation these...
Islet neogenesis associated protein (INGAP) increases islet mass and insulin secretion in neonatal adult rat islets. In the present study, we measured short- long-term effects of INGAP-PP (a pentadecapeptide having 104–118 amino acid sequence INGAP) upon expression phosphorylation components PI3K, MAPK cholinergic pathways, on secretion. Short-term exposure islets to (90 s, 5, 15, 30 min) significantly increased Akt1 -Ser473 MAPK3/1 -Thr202/Tyr204 also acutely from perifused with 2 20 mM...
The aim of this work was to develop an efficient, biodegradable, biocompatible and safe controlled release system using insulin-loaded poly(epsilon-caprolactone) (PCL) nanoparticles. PCL nanoparticles were prepared by double emulsion method (water-in-oil-in-water) Pluronic F68 as emulsifier. Using the a high insulin encapsulation efficiency (90.6 +/-1.6%) with zeta potential -29 +/-2.7 mV average particle size 796 +/-10.5 nm obtained. Insulin-loaded showed no toxicity MIN6 cells. Insulin...
During pregnancy, the maternal endocrine pancreas undergoes, as a consequence of placental lactogens and prolactin (PRL) action, functional changes that are characterized by increased glucose-induced insulin secretion. After delivery, rapidly returns to non-pregnant state, which is mainly attributed serum levels glucocorticoids (GCs). Although GCs known decrease secretion counteract PRL mechanisms for these effects poorly understood. We have previously demonstrated signal transducer...
To demonstrate the role of islet glucokinase, glucose metabolism, and intracellular insulin mediators in enhancing effect neogenesis-associated protein pentadecapeptide (INGAP-PP) on glucose-induced secretion.Islets from normal rats were cultured for 4 days absence or presence 10 μg/mL INGAP-PP, with/without Wortmannin LY294002. Islets incubated with different concentrations to measure secretion content, hexokinase glucokinase activity, oxidation utilization, receptor, receptor substrate...
Abstract Aging is associated with glucose metabolism disturbances, such as insulin resistance and hyperinsulinemia, which contribute to the increased prevalence of type 2 diabetes (T2D) its complications in elderly population. In this sense, some bile acids have emerged new therapeutic targets treat TD2, well metabolic disorders. The taurine conjugated acid, tauroursodeoxycholic acid (TUDCA) improves homeostasis T2D, obesity, Alzheimer's disease mice model. However, effects aged not been...