A5039629478- Pancreatic function and diabetes
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Metabolism, Diabetes, and Cancer
- Diabetes Management and Research
- Natural Antidiabetic Agents Studies
- Fluoride Effects and Removal
- Bone and Dental Protein Studies
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Birth, Development, and Health
- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Agricultural and Food Sciences
- Exercise and Physiological Responses
- Muscle Physiology and Disorders
- Phytochemistry Medicinal Plant Applications
- Sleep and Wakefulness Research
- Connexins and lens biology
- Advanced Glycation End Products research
- Diabetes Treatment and Management
- Dental Health and Care Utilization
Universidade Estadual Paulista (Unesp)
2010-2024
Fundação Faculdade de Odontologia
2024
Universidade de São Paulo
2008-2024
Universidade Federal de Santa Catarina
2012
Fundação de Apoio à Universidade Federal de São Paulo
2007
Universidade Estadual de Campinas (UNICAMP)
1997-2007
Activation of insulin signaling and cell cycle intermediates is required for adult beta-cell proliferation. Here, we report a model to study proliferation in living rats by administering three different doses dexamethasone (0.1, 0.5, 1.0 mg/kg ip, DEX 0.1, 1.0, respectively) 5 days. Insulin sensitivity, secretion, histomorphometric data were investigated. Western blotting was used analyze the levels proteins related control growth. rats, which present moderate hyperglycemia marked...
Objectives: We have analyzed the peripheral insulin and glucose sensitivity in vivo, islet function ex vivo rats with different degrees of resistance induced by dexamethasone (DEX). Methods: Dexamethasone, concentrations 0.1 (DEX 0.1), 0.5 0.5), 1.0 mg/kg body weight 1.0) was administered daily, intraperitoneally, to adult Wistar for 5 days, whereas controls received saline. Results: Dexamethasone treatment a dose-dependent manner. At end treatment, only DEX showed significant increase...
Abstract Although glucocorticoids are widely used as antiinflammatory agents in clinical therapies, they may cause serious side effects that include insulin resistance and hyperinsulinemia. To study the potential functional adaptations of islet Langerhans to vivo glucocorticoid treatment, adult Wistar rats received dexamethasone (DEX) for 5 consecutive days, whereas controls (CTL) only saline. The analysis release freshly isolated islets showed an enhanced secretion response glucose...
Long-term dexamethasone therapy may induce peripheral insulin resistance (IR), which in turn elicits increased beta-cell function and proliferation. However, whether such adaptive compensations occur during short-term treatment with is unclear. Here, we compared morphofunctional parameters endocrine pancreas after short- long-term administration. Groups of rats received daily i. p. injection 1 mg/kg b. w. for (DEX-1), 3 (DEX-3), or 5 consecutive days (DEX-5), whilst control were...
Augmented glucose-stimulated insulin secretion (GSIS) is an adaptive mechanism exhibited by pancreatic islets from insulin-resistant animal models. Gap junction proteins have been proposed to contribute islet function. As such, we investigated the expression of connexin 36 (Cx36), 43 (Cx43), and glucose transporter Glut2 at mRNA protein levels in dexamethasone (DEX)-induced rats. Study rats received daily injections DEX (1 mg/kg body mass, i.p.) for 5 days, whereas control (CTL) saline...
Abstract: In this study, we evaluated the effects of obesity and insulin resistance induced by a high‐fat diet on prostate morphophysiology, focusing cell proliferation, expression androgen (AR) estrogen receptors (ER) proteins signaling pathway. Adult male Wistar rats were fed (20% fat) for 15 weeks, whereas control animals received balanced (4% fat). Both groups then divided treated 2 weeks with 1 mg/kg body weight/day aromatase inhibitor letrozole or vehicle only. The ventral was analyzed...
Species of Myrcia are used by indigenous people and in traditional communities Brazil for the treatment Diabetes mellitus. We investigated hypoglycemic effect extract leaves bella diabetic mice. The chemical fingerprinting 70% EtOH characterized as main constituents flavonoid aglycones, flavonoid-O-glycosides, acylated flavonoid-O-glycosides derivatives quercetin myricetin. Mice were treated with saline or M. (300 600 mg/Kg b.w.) 14 days. Body weight water food intake measured every day....
It is well known that glucocorticoids induce peripheral insulin resistance in rodents and humans. Here, we investigated the structural ultrastructural modifications, as proteins involved beta-cell function proliferation, islets from insulin-resistant rats. Adult male Wistar rats were made resistant by daily administration of dexamethasone (DEX; 1mg/kg, i.p.) for five consecutive days, whilst control (CTL) received saline alone. Structure analyses showed a marked hypertrophy DEX with an...
Abstract Aim: Glucocorticoid administration induces insulin resistance (IR) and enhances islet mass secretion in rodents humans. Here, we analysed whether these effects are still present after the interruption of dexamethasone treatment. Methods: Adult Wistar rats were distributed into CTL (daily injection saline for five consecutive days), DEX 1 mg kg −1 body wt days) 10 (5 days treatment, followed by a period without dexamethasone). Results: In vivo experiments indicated that marked...
Endurance training has been shown to increase pancreatic β-cell function and mass. However, whether exercise modulates growth survival pathways signaling is not completely understood. This study investigated the effects of on apoptotic markers levels in rat islets. Male Wistar rats were randomly assigned 8-wk endurance or a sedentary control group. After that, islets isolated; gene expression total content phosphorylation several proteins related as well main antioxidant enzymes determined...
Pancreatic β cells are very sensitive to reactive oxygen species (ROS) and this might play an important role in cell death diabetes. Dexamethasone is a synthetic diabetogenic glucocorticoid, which impairs pancreatic function. Therefore we investigated the toxicity of dexamethasone RINm5F insulin-producing its dependence on expression level antioxidant enzyme catalase, inactivates hydrogen peroxide. This was correlated with oxidative stress death. An increased generation ROS observed...
This study investigated how proteins of the insulin signaling cascade could modulate resistance after dexamethasone (Dexa) treatment and aerobic training. Rats were distributed into 4 groups: sedentary control (SC), sedentary+Dexa (SD), trained (TC), trained+Dexa (TD), underwent training for 70 days or remained sedentary. Dexa was administered during last 10 (1 mg · kg<sup>−1</sup> per day i. p.). After days, an intraperitoneal glucose tolerance test (ipGTT) performed. Protein levels IRS-1,...
The fruit bat Artibeus lituratus absorbs large amounts of glucose in short periods time and maintains normoglycemia even after a prolonged starvation period. Based on these data, we aimed to investigate various aspects related with homeostasis analyzing: blood insulin levels, intraperitoneal tolerance tests (ipGTT ipITT), glucose-stimulated secretion (2.8, 5.6 or 8.3 mmol/L glucose) pancreas fragments, cellular distribution beta cells, the amount pAkt/Akt pectoral muscle liver. Blood levels...
Previous studies by our research group using a model of insulin resistance induced dexamethasone (DEX) showed that in the rat ventral prostate there was epithelial and smooth muscle cell atrophy were also alterations fibroblasts. Proteins signalling pathway are known to be very important for proliferation development. Thus, we investigated this correlated findings with expression glucocorticoid (GR) androgen (AR) receptors. Insulin adult male Wistar rats injection DEX (1 mg/kg, ip 5...
Chronic administration of glucocorticoids (GC) leads to characteristic features type 2 diabetes in mammals.The main action dexamethasone target cells occurs through modulation gene expression, although the exact mechanisms are still unknown.We therefore investigated expression profile pancreatic islets from rats treated with using a cDNA array screening analysis.The selected genes and proteins involved mitochondrial apoptosis was further analyzed by PCR immunoblotting.Insulin, triglyceride...
During pregnancy, the maternal endocrine pancreas undergoes, as a consequence of placental lactogens and prolactin (PRL) action, functional changes that are characterized by increased glucose-induced insulin secretion. After delivery, rapidly returns to non-pregnant state, which is mainly attributed serum levels glucocorticoids (GCs). Although GCs known decrease secretion counteract PRL mechanisms for these effects poorly understood. We have previously demonstrated signal transducer...
Bauhinia L. species, including holophylla (Bong.) Steud. (Fabaceae), have traditionally been used to treat diabetes. is a complex botanical genus, and the indiscriminate use of diverse species reflected in experimental divergence their medicinal potential.The hypoglycaemic hypolipidaemic effects, molecular mechanism action phytochemical properties an authentic extract B. leaves were evaluated.A study 70% EtOH was performed using FIA-ESI-IT-MS/MSn HPLC-PAD-ESI-IT-MS. The (200 or 400 mg/kg...
Fluoride (F) has been widely used to control dental caries, and studies suggest beneficial effects against diabetes when a low dose of F is added the drinking water (10 mgF/L). Objectives This study evaluated metabolic changes in pancreatic islets NOD mice exposed doses main pathways altered by treatment. Methodology In total, 42 female were randomly divided into two groups, considering concentration administered for 14 weeks: 0 or 10 mgF/L. After experimental period, pancreas was collected...