Long-term dexamethasone therapy may induce peripheral insulin resistance (IR), which in turn elicits increased beta-cell function and proliferation. However, whether such adaptive compensations occur during short-term treatment with is unclear. Here, we compared morphofunctional parameters endocrine pancreas after short- long-term administration. Groups of rats received daily i. p. injection 1 mg/kg b. w. for (DEX-1), 3 (DEX-3), or 5 consecutive days (DEX-5), whilst control were...

Abstract: In this study, we evaluated the effects of obesity and insulin resistance induced by a high‐fat diet on prostate morphophysiology, focusing cell proliferation, expression androgen (AR) estrogen receptors (ER) proteins signaling pathway. Adult male Wistar rats were fed (20% fat) for 15 weeks, whereas control animals received balanced (4% fat). Both groups then divided treated 2 weeks with 1 mg/kg body weight/day aromatase inhibitor letrozole or vehicle only. The ventral was analyzed...

Summary Recent studies have shown a positive association of cancer and obesity, but the morphological molecular mechanisms involved in this relationship are still unknown. This study analysed impact long‐term obesity on rat prostate, focusing stromal changes. Male adult Wistar rats were treated with high‐fat diet to induce while control group received balanced diet. After 30 weeks feeding, ventral prostate was by immunohistochemistry for cell proliferation, smooth muscle α‐actin, vimentin,...

This study compares the impact of obesogenic environment (OE) in six different periods development on sperm parameters and testicular structure adult rats their correlations with sex steroid metabolic scenario. Wistar were exposed to OE during gestation (O1), gestation/lactation (O2), from weaning adulthood (O3), lactation (O4), sexual maturity (O5), after maturation (O6). was induced by a 20% fat diet, control groups fed balanced diet (4% fat). Serum leptin levels adiposity index indicate...

This study evaluated the impact of a high-fat diet (HFD) during different stages rat life, associated or not with maternal obesity, on content sex steroid hormones and morphophysiology Leydig cells. The following periods development were examined: gestation (O1), lactation (O2), from weaning to adulthood (O3), (O4), (O5), after sexual maturation (O6). HFD contained 20% unsaturated fat, whereas control had 4% fat. Maternal obesity was induced by feeding 15 weeks before mating. All groups...

This study determined the phases of sexual development male Mongolian gerbil (Meriones unguiculatus) based on an integrative analysis testicular morphology, hormonal data and sperm parameters. Male gerbils were analysed at 1, 7, 14, 21, 28, 35, 42, 50, 60, 70, 90, 100 120 days age. Body, epididymal weights increased up to Day 60 respectively. The impuberal phase, characterised by presence gonocytes, extended until 14. prepubertal period lasted when puberty was achieved a drastic increase in...

Melatonin may be used as an antioxidant in therapy against systemic sequelae caused by oxidative stress diabetes. However, melatonin has a major role regulating reproductive activity, its consequence on parameters under diabetes needs to better clarified. We have studied whether prior and concomitant treatment of juvenile Wistar rats with low doses interferes damage induced experimental after 1 8 weeks. The consequences administration since weaning healthy at adulthood were also evaluated....

BACKGROUND Experimental data indicate that high-fat diet (HFD) may alter proliferative activity and prostate health. However, the consequences of HFD exposure during different periods ontogenetic development on histophysiology remain to be elucidated. Herein, we compare influence obesogenic environment (OE) due maternal obesity at life nuclear receptors frequency in rat ventral a possible relationship with metabolic hormonal alterations. METHODS Male Wistar rats (19 weeks old), treated...

In this study, we evaluated whether maternal obesity (MO) affects testis development and gonocyte differentiation in the rat from 0.5 to 14.5 postnatal days. Male Wistar rats were used at 0.5, 4.5, 7.5, days post partum (dpp). These born obese mothers, previously fed with a high-fat diet (20% saturated fat), for 15 weeks, or normal mothers that had received balanced murine (4% lipids). MO did not affect weight histology birth but changed migratory behavior of gonocytes. The density relocated...

BACKGROUND TNF‐α is a key cytokine involved in prostate carcinogenesis and mediated by the receptor type 1 (TNFR‐1). This triggers two opposite pathways: cell death or survival presents protective stimulator role cancer. Thus, purpose of this study was to evaluate TNF signaling chemically induced mice. METHODS C57bl/6 wild (WT) p55 TNFR‐1 knockout mice (KO) were treated with mineral oil (control) N‐methyl N‐nitrosurea (MNU) association testosterone (MNU+T, single injection 40 mg/kg weekly 2...

Considering the increasing consumption of saturated fat and glucose in diets worldwide its possible association to carcinogenesis, this investigation analysed proliferation profile nonmalignant human prostate epithelial cells after exposure elevated levels glucose. PNT1A were cultured with palmitate (100 or 200 μM) and/or (450 mg/dl) for 24 48 h. Treated evaluated viability test cell (MTS assay). AKT AMPK phosphorylation status by Western blotting. After h high-fat alone associated...

Phthalate esters are endocrine disrupters that can affect the development of testis in a species-specific manner. However, their interference male gonads Mongolian gerbil is unknown. The aim present study was to evaluate whether gestational exposure di-n-butyl phthalate (DBP) interferes with during first six weeks life. Males were evaluated at 1, 7, 14, 28, 35 and 42 days age an untreated (control) group or groups exposed from 8 23 gestation DBP (100 mg kg−1 day−1 mineral oil) vehicle by...

Abstract This study describes the neonatal differentiation of Mongolian gerbil gonocytes, focusing on relationship between its relocation to basement membrane, apoptosis and postrelocation changes also distribution androgen receptors (AR). Testes gerbils from 1 35 days age (d) were examined by high resolution light microscopy immunocytochemistry for proteins PCNA, VASA, AR as well TUNEL method. Gonocytes quantified according degree into nonrelocated, relocating relocated. Most them found in...

The effects of intrauterine exposure to 17β-oestradiol (E2) are well studied for the male prostate and there accumulating evidences that high dosages leads a hypomorphic development. However, is lack information about E2 in rodent females, such research becomes relevant view presence functional proportion women, morphophysiological similarities between female rodents women. This study uses histochemical, immunohistochemical, immunofluorescence three-dimensional (3D) reconstruction techniques...

Abstract Information on postnatal Leydig cell (LC) differentiation in the Mongolian gerbil has been unavailable. Therefore, current investigation was designed to examine LC lineage differentiationin this rodent, from birth adulthood. Gerbil testes at 1 day, 1–7 weeks (w), 2 and 3 months of age were conventionally processed by light transmission electron microscopy. Immunocytochemistry for specific markers steroidogenic enzymes, namely 3β‐hydroxysteroid dehydrogenase (3β‐HSD)...

Maternal obesity plus high-fat diet in breastfeeding induces stromal hyperplasia and diffuse acinar atrophy the rat prostate at aging, related to dyslipidemia testosterone reduction. The high-lipid nutritional environment from intrauterine throughout life favors development of prostatic intraepithelial neoplasia aggravated degenerative alterations gland.Maternal (HFD) affect permanently histophysiology adulthood, but consequences during aging are unknown. Here, we evaluated middle-aged rats...

A depressão pós-parto (DPP) é definida como um transtorno mental com alta prevalência, acompanhada de alterações emocionais, cognitivas, comportamentais e físicas. Geralmente inicia entre a quarta oitava semana após o parto (por vezes mais tarde, mas ainda dentro do primeiro ano) pode persistir por ano. O estudo trata-se uma revisão bibliográfica, onde visou identificar fatores associados à DPP, incluindo risco, manifestações clínicas, diagnóstico tratamento. partir das análises os dados...