Activation of insulin signaling and cell cycle intermediates is required for adult beta-cell proliferation. Here, we report a model to study proliferation in living rats by administering three different doses dexamethasone (0.1, 0.5, 1.0 mg/kg ip, DEX 0.1, 1.0, respectively) 5 days. Insulin sensitivity, secretion, histomorphometric data were investigated. Western blotting was used analyze the levels proteins related control growth. rats, which present moderate hyperglycemia marked...

To assess the efficacy of heterologous fibrin biopolymer (HFB) in promoting alveolar bone healing after tooth extraction rats.

Fluoride (F) is a potent anti-cariogenic element, but when ingestion excessive, systemic toxicity may be observed. This can occur as acute or chronic responses, depending on both the amount of F and time exposure. The present study identified profile protein expression possibly associated with F-induced hepatotoxicity. Weanling male Wistar rats (three-weeks old) were divided into three groups treated drinking water containing 0, 5 50 mg/L for 60 days (n=6/group). At this point, serum livers...

In recent years, important advances have occurred in the determination of diagnostic criteria for disease diabetes mellitus and new strategies its treatment. The purpose this research was to develop a method diagnosis by microscopic cytomorphometric analyses oral epithelium.The smears were obtained from three distinct sites: buccal mucosa (cheek), tongue dorsum, floor mouth 10 control individuals type II diabetic patients. stained with Papanicolaou EA-36 solution. nuclear (NA) cytoplasmic...

Periodontal disease in diabetic patients presents higher severity and prevalence; increased of ligature-induced periodontal has been verified rats. However, absence aggressive stimuli such as ligatures, the influence diabetes on rat tissues is incompletely explored. The aim this study was to evaluate establishment progression diseases rats only with induction.Diabetes induced Wistar (n = 25) by intravenous administration alloxan (42 mg/kg) were analyzed at 1, 3, 6, 9 12 months after...

Abstract Objective: To histomorphometrically investigate the repair of critical size defects (CSDs) and bone augmentation in cranial walls using block sintered bovine‐derived anorganic (sBDAB) graft. Material methods: Forty guinea‐pigs were divided into test ( n =20) CSD control groups. In each animal, a full‐thickness defect with 9.5 mm diameter was made frontal bone. The filled an sBDAB soaked blood group clot group. skulls collected at 0 h =2) 30, 90 180 days =6/group period)...

Background: Aggregatibacter actinomycetemcomitans (previously Actinobacillus ) is a Gram‐negative bacterium present in the oral cavity and usually associated with localized aggressive periodontitis. Isolated antigens from A. can activate innate immune cells through Toll‐like receptors (TLRs), which are molecules that recognize structural components conserved among microorganisms. In this study, we evaluate role of TLR2 recognition actinomycetemcomitans. Methods: Macrophages neutrophils...

Diabetes mellitus comprises a heterogeneous group of disorders with the main feature hyperglycemia. Chronic hyperglycemia increases severity periodontal disease via an exacerbated inflammatory response, activated by advanced glycation end products and their receptor, RAGE. Therefore, anti-inflammatory agents represent potential inhibitors this pathological interaction. In particular, green tea has been shown to possess properties mediated its polyphenol content. Objectives: This study...

Abstract The bone‐induction capacity of a porous biphasic calcium phosphate (pBCP) using heterotopic implantation in mouse (mHI‐model) and its efficacy as substitute for autograft mandibular critical‐size defect rabbit (rabMCSD‐model) was investigated. In mHI‐model, pBCP implanted into the thigh muscles bone formation histomorphometrically immunohistochemically evaluated. rabMCSD‐model, 13 mm defects were treated with or repair comparatively evaluated by radiographic histomorphometric...

The role of fluoride (F) in oxidative stress is well reported, but its effects on the lipid metabolism has not been completely explored Background Here, we evaluated relationship diet and F-induced to liver rats eating normocaloric or hypercaloric diets for two time periods (20 60 days). Methods Seventy-two 21-day-old Wistar were divided into 2 groups (n = 36) based type they eating; each these was then further another 18) either 20 days, a total four groups. Each 3 subgroups 6...

Vascular endothelial growth factor (VEGF) is a macromolecule of importance in inflammation that has been implicated periodontitis. The aims this study were to investigate VEGF expression during the progression periodontal disease and evaluate effect preferential cyclooxygenase (COX)-2 inhibitor meloxicam on alveolar bone loss experimentally induced periodontitis.A total 120 Wistar rats randomly separated into groups 1 (control) 2 (meloxicam, 3 mg/kg/day, intraperitoneally, for 3, 7, 14, or...

The pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features this disease should be developed. OBJECTIVE: In study, the evolution experimentally induced infection in rat sockets characterized, which leads to clinical signs suppurative with remarkable wound healing disturbs. MATERIAL AND METHODS: Non-infected (Group I) infected Rattus novergicus II) were histometrically evaluated regarding kinetics alveolar healing. addition, characterization...

The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance plasticity. Xenogenic grafts of bovine cancellous origin are particularly interesting due to their biologically designed porous structure that enhance both cellular vascular invasion. purpose this study was evaluate the tissue response induced by macrogranular anorganic implanted in rat subcutaneous tissue. Forty rats were assigned 2 groups, as follows: control group received empty...

Abstract Objective: To evaluate the repair of critical‐size bone defects in rats treated with demineralized bovine (DBB) compared autogenous (AB). Material and method: A defect 8 mm diameter was created calvaria 50 Rattus norvegicus , either DBB or AB. Sub‐groups five each group were killed at 7, 14, 21, 30 90 days post‐operatively, skulls removed processed histologically. Histological sections stained hematoxylin eosin. Result: analysis showed complete closure new AB, substitution...

Diabetes mellitus is a heterogeneous group of disorders, in which hyperglycemia main feature. The objective was to evaluate the involvement RAGE, inflammatory cytokines, and metalloproteinases spontaneous periodontitis triggered by diabetes induction. Immunohistochemical procedures for MMP-2, MMP-9, TNF-α, IL-1β, IL-6, RANKL, RAGE were performed rats after 1, 3, 6, 9, 12 months Total DNA extracted from paraffin-embedded tissues evaluated Real-TimePCR 16S total bacterial load specific...

To evaluate two treatment modalities of dry socket in rats and to discuss the first findings molecular analysis this experimental model.84 underwent a tooth extraction were divided 4 groups: I-uninfected (control), II-infected without any treatment, III-infected treated with irrigation 2% sodium iodide 3% hydrogen peroxide solution, IV-infected submitted curettage, physiological saline solution fulfilled metronidazole paste as base. The groups subdivided postoperative sacrifice periods:...

Few studies have demonstrated the pathologic reactions yielded by smoke inhalation on airway in rats.The aim of this study was to analyze possible histopathological effects produced chronic cigarette vocal folds rats.Experimental.36 male rats (Rattus norvergicus Wistar strain), aged 60 days, were kept cages and exposed 10 cigarettes lit 3 times a day, 7 days week, for periods 25, 50 75 their respective controls. Thereafter animals killed larynxes dissected submitted histological processing...

Given that tobacco smoking habit is a risk factor for periodontal diseases, the aim of this study was to compare clinical aspects between smokers and non-smokers. The status were assessed in 55 patients, 29 26 non-smokers, aged 30 50 years, with mean age 40. parameters used were: probing depth (PD), plaque index (PI), gingival (GI), attachment level (CAL), recession (GR) bleeding (GBI) arches (upper lower ) teeth (anterior posterior). Tooth loss also evaluated both groups. Multiple...