A5046441902- Fluoride Effects and Removal
- Oral microbiology and periodontitis research
- Bone and Dental Protein Studies
- Salivary Gland Disorders and Functions
- Dental Erosion and Treatment
- Heavy Metal Exposure and Toxicity
- Mercury impact and mitigation studies
- Dental Health and Care Utilization
- Folate and B Vitamins Research
- Anesthesia and Neurotoxicity Research
- Alcohol Consumption and Health Effects
- Trace Elements in Health
- Laser Applications in Dentistry and Medicine
- Dental Trauma and Treatments
- Nutrition and Health in Aging
- Selenium in Biological Systems
- S100 Proteins and Annexins
- Digestive system and related health
- Aluminum toxicity and tolerance in plants and animals
- Pancreatic function and diabetes
- Temporomandibular Joint Disorders
- Dental Anxiety and Anesthesia Techniques
- Diet and metabolism studies
- Medical and Biological Ozone Research
- Cancer, Hypoxia, and Metabolism
Universidade de São Paulo
2015-2025
Fundação Faculdade de Odontologia
2018-2024
Universidade Brasil
2023
Centre Hospitalier de Douai
2023
Universidade São Francisco
2015
The excessive fluoride (F) exposure is associated with damage to cellular processes of different tissue types, due changes in enzymatic metabolism and breakdown redox balance. However, few studies evaluate doses F compatible human consumption. Thus, this study evaluated the effects chronic sodium (NaF) on peripheral blood mice from evaluation biochemical parameters. animals were divided into three groups (n = 10) received concentrations NaF drinking water for 60 days: 0 mg/L F, 10 50 F. was...
Long-term exposure to high concentrations of fluoride (F) can damage mineralized and soft tissues such as bones, liver, kidney, intestine, nervous system adult rats. The permeability the blood–brain barrier placenta F during pregnancy lactation may be critical neurological development. Therefore, this study aimed investigate effects on molecular processes oxidative biochemistry offspring rats' hippocampus. Pregnant Wistar rats were randomly assigned into 3 groups in accordance with drinking...
<b><i>Introduction:</i></b> This study investigated the changes in acquired enamel pellicle (AEP) proteome when this integument is formed vivo after treatment with sugarcane-derived cystatin (CaneCPI-5), hemoglobin (HB), and a statherin-derived peptide (StN15), or their combination then exposed to an intrinsic acid challenge. The effectiveness of these treatments preventing erosion was also evaluated. <b><i>Methods:</i></b> Ten volunteers,...
Investigation of biochemical and morphological parameters underlying the cognitive dysfunction after MeHg exposure.
Gastrointestinal symptoms are the first signs of fluoride (F) toxicity. In present study, jejunum rats chronically exposed to F was evaluated by proteomics, as well morphological analysis. Wistar received water containing 0, 10 or 50 mgF/L during 30 days. HuC/D, neuronal Nitric Oxide (nNOS), Vasoactive Intestinal Peptide (VIP), Calcitonin Gene Related (CGRP), and Substance P (SP) were detected in myenteric plexus immunofluorescence. The density nNOS-IR neurons significantly decreased...
The development of new approaches allowing for the early assessment COVID-19 cases that are likely to become critical and discovery therapeutic targets urgently required. In this prospective cohort study, we performed proteomic laboratory profiling plasma from 163 patients admitted Bauru State Hospital (Brazil) between 4 May 2020 July 2020. Plasma samples were collected upon admission routine analyses shotgun quantitative label-free proteomics. Based on course disease, divided into three...
<b><i>Introduction:</i></b> The identification of acid-resistant proteins, including hemoglobin (Hb), within the acquired enamel pellicle (AEP) led to proposition “acquired engineering” concept, which involves modification AEP by incorporating specific presenting a novel strategy prevent dental demineralization. <b><i>Objective:</i></b> Combining in vivo and vitro proof-of-concept protocols, we sought reveal impact engineering with Hb protein...
The role of fluoride (F) in oxidative stress is well reported, but its effects on the lipid metabolism has not been completely explored Background Here, we evaluated relationship diet and F-induced to liver rats eating normocaloric or hypercaloric diets for two time periods (20 60 days). Methods Seventy-two 21-day-old Wistar were divided into 2 groups (n = 36) based type they eating; each these was then further another 18) either 20 days, a total four groups. Each 3 subgroups 6...
Saliva contains numerous proteins and peptides, each of them carries a number biological functions that are very important in maintaining the oral cavity health also yields information about both local systemic diseases. Currently, proteomic analysis is basis for large-scale identification these discovery new biomarkers distinct Objective This study compared methodologies to extract salivary analysis. Material Methods samples were collected from 10 healthy volunteers. In first test,...
Although the literature does not provide evidence of health risks from exposure to fluoride (F) in therapeutic doses, questions remain about effects long-term and high-dose use on function central nervous system. The objective this study was investigate effect F at levels similar those found areas artificial water fluoridation endemic fluorosis biochemical, proteomic, cell density, functional parameters associated with cerebellum. For this, mice were exposed containing 10 mg F/L or 50 (as...
<b><i>Objective:</i></b> Saliva is the most important biological factor to protect against erosive tooth wear (ETW). Gastroesophageal reflux disease (GERD) patients have an increased risk of ETW due frequent presence intrinsic acids in oral cavity. Remarkably, not all GERD suffer from ETW, which might be differences composition saliva. <b><i>Methods:</i></b> This study compared proteomic profile saliva (1) with and (basic examination, BEWE,...
The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml−1 CaneCPI-5, 0.1 or 0.5 CaneCPI-5. They incubated inoculum (human saliva + McBain's saliva) the first 8 h. From then until end experiment, was exposed to McBain sucrose and, once a day, 5 days, they solutions. At experimental period, resazurin viable plate count assays performed. Enamel...
Mercury is a severe environmental pollutant with neurotoxic effects, especially when exposed for long periods. Although there are several evidences regarding mercury toxicity, little known about inorganic (IHg) species and cerebellum, one of the main targets associated neurological symptomatology mercurial poisoning. Besides that, global proteomic profile assessment valuable tool to screen possible biomarkers elucidate molecular neurotoxicity; however, literature still scarce. Thus, this...
Abstract Fluoride is added to water due its anticariogenic activity. However, natural presence in soils and reservoirs at high levels, it could be a potential environmental toxicant. This study investigated whether prolonged exposure fluoride from adolescence adulthood—at concentrations commonly found artificially fluoridated fluorosis endemic areas—is associated with memory learning impairments mice, assessed the molecular morphological aspects involved. For this endeavor, 21-days-old mice...
The objective of this study was to compare the protein profile acquired enamel pellicle (AEP) formed in vivo patients with or without gastroesophageal reflux disease (GERD), and erosive tooth wear (ETW). Twenty-four volunteers were divided into 3 groups: 1) GERD ETW; 2) 3) control (without GERD). AEP 120 min after prophylaxis collected from lingual/palatal surfaces. samples subjected mass spectrometry (nLC-ESI-MS/MS) label-free quantification by Protein Lynx Global Service software. A total...
Objective This in vitro study evaluated the effect of commercial whitening dentifrices on erosive tooth wear (ETW) bovine enamel samples, comparison with regular dentifrices. Methodology Sixty crowns were embedded acrylic resin, polished and then had their baseline profile determined. They randomly assigned to 5 groups (n=12/group), according type dentifrice be tested: GI – Crest Anti-cavity Regular; GII 3D White; GIII Colgate Total 12 Clean Mint; GIV Optic GV Placebo (negative control,...
Lead (Pb) is an environmental contaminant that presents a high risk for human health. We aimed to investigate the possible alterations triggered by exposure Pb acetate long period in motor performance and relationship with biochemical, proteomic morphological cerebellum of rats. Male Wistar rats were exposed 55 days, at 50 mg/Kg acetate, control animals received distilled water. Open field (OF) rotarod tests; biochemistry parameters (MDA nitrite); staining/immunostaining Purkinje cells (PC),...