A5062600991- Cleft Lip and Palate Research
- SARS-CoV-2 and COVID-19 Research
- Inflammatory mediators and NSAID effects
- COVID-19 Clinical Research Studies
- S100 Proteins and Annexins
- Biosensors and Analytical Detection
- Craniofacial Disorders and Treatments
- dental development and anomalies
- SARS-CoV-2 detection and testing
- Neuropeptides and Animal Physiology
- Oral microbiology and periodontitis research
- Blood Coagulation and Thrombosis Mechanisms
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
- Vitamin D Research Studies
- Endodontics and Root Canal Treatments
- Receptor Mechanisms and Signaling
- Environmental Sustainability and Education
- Ethics in Clinical Research
- Pancreatitis Pathology and Treatment
- Geochemistry and Elemental Analysis
- Congenital Ear and Nasal Anomalies
- Magnesium in Health and Disease
- Magnesium Oxide Properties and Applications
- Biological Research and Disease Studies
Universidade de São Paulo
2017-2023
Universidade do Porto
2021-2023
Instituto Butantan
2023
The development of new approaches allowing for the early assessment COVID-19 cases that are likely to become critical and discovery therapeutic targets urgently required. In this prospective cohort study, we performed proteomic laboratory profiling plasma from 163 patients admitted Bauru State Hospital (Brazil) between 4 May 2020 July 2020. Plasma samples were collected upon admission routine analyses shotgun quantitative label-free proteomics. Based on course disease, divided into three...
This study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six twelve months using five different extraction protocols: protocol 1 - Oragene™ commercial kit, 2 QIAamp mini 3 ammonium acetate, 4 Instagene™ Matrix 5 diluted 1:1 proteinase K 1% SDS. Briefly, analyzed spectrophotometry, electrophoresis PCR.Results indicated time spent in storage typically decreased with exception 1. The purity generally not affected by times based...
Abstract Background The initiation and progression of periodontitis might involve a local renin‐angiotensin system in periodontal tissue. This study hypothesized that Losartan treatment could promote protection to rats submitted experimental (EP) by attenuating alveolar bone loss due reduction inflammatory cytokines, better reactive oxidant species regulation maintenance the balance between formation resorption factors. Methods One hundred thirty were EP with silk suture thread (4.0) placed...
Abstract Objective Subjects with cleft lip and palate (CLP) present high prevalence of dental agenesis. Among candidate genes for these phenotypes is IRF6 . However, genetic studies do not analyze agenesis as a phenotype associated cleft. Therefore, we investigated the frequency rare novel variations in subjects non‐syndromic unilateral (NSUCLP), without Methods Genomic DNA samples 100 NSUCLP 50 controls were sequenced. mutational screening was conducted by direct sequencing. Results Ten new...
Abstract Background Oral fibroblast immunological responses to bacterial stimuli are well known. However, there few studies about pulp fibroblasts from deciduous teeth (HDPF) responses, which important for the treatment of infections in children. The aim this study was evaluate expression and production inflammatory cytokines chemokines by HDPF when challenged with antigens normally present advanced caries lesions. Methods Triplicate 4 children ( n = 4; 2 boys girls) were cultured explant...
Activation of renin–angiotensin system (RAS) plays a role in bone deterioration associated with metabolic disorders, via increased Angiotensin II (AngII) targeting type 1 receptor/Angiotensin 2 receptor (AT1R/AT2R). Despite the wide data availability, RAS remains controversial. This study analyzes feasibility using embryonic chick femur organotypic model to address AngII/AT1R/AT2R axis bone, which is an application not yet considered. Embryonic day-11 femurs were cultured ex vivo for 11 days...
Considering the role of magnesium in bone metabolism and increasing relevance plant-mediated green-synthesis, this work compares cytocompatibility hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)2, or a rosehip (RH) aqueous extract, Mg(OH)2RH. The NPs were evaluated for dose- time-dependent effects on human osteoblastic osteoclastic response, due to direct involvement two cell types metabolism. Mg(OH)2 presented nanoplatelet-like morphology (mean diameter ~90 nm)...
Abstract The development of new approaches that allow early assessment which cases COVID-19 will likely become critical and the discovery therapeutic targets are urgent demands. In this cohort study, we performed proteomic laboratorial profiling plasma from 163 patients admitted to Bauru State Hospital (Bauru, SP, Brazil) between May 4 th July , 2020, who were diagnosed with by RT-PCR nasopharyngeal swab samples. Plasma samples collected upon admission for routine laboratory analyses shotgun...
Angiotensin II (Ang II) plays a role in several inflammatory diseases and is capable of inducing the release mediators cell types. The objective was to investigate potential Ang induce mRNA expression by cultured primary human periodontal cells. A synergistic effect co-treatment with IL1β on also explored. Primary cultures fibroblast-like cells isolated from gingival ligament tissues 3 subjects were established using explant techniques. Immunophenotyping STRO-1, type 1 receptor...
Angiotensin II (Ang II) plays a role in several inflammatory diseases and is capable of inducing the release mediators cell types. The objective was to investigate potential Ang induce mRNA expression by cultured primary human periodontal cells. A synergistic effect co-treatment with IL1β on also explored. Primary cultures fibroblast-like cells isolated from gingival ligament tissues 3 subjects were established using explant techniques. Immunophenotyping STRO-1, AT1 AT2 receptors performed...
Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription (RT-PCR) a transparent accessible qualitative method for SARS-CoV-2 detection from nasopharyngeal oral pharyngeal samples with potential generate actionable data more quickly, at lower cost, fewer experimental resources than full RT-qPCR. This study...
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Abstract Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription (RT-PCR) an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal oral pharyngeal samples with potential generate actionable data more quickly, at a lower cost, fewer experimental resources than full RT-qPCR. This study...