A5019339569- Influenza Virus Research Studies
- Infective Endocarditis Diagnosis and Management
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Nosocomial Infections in ICU
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Healthcare cost, quality, practices
- Silk-based biomaterials and applications
- Mycobacterium research and diagnosis
- Vasculitis and related conditions
- Infections and bacterial resistance
- Microscopic Colitis
- Antifungal resistance and susceptibility
- Acne and Rosacea Treatments and Effects
- Gut microbiota and health
- Microbial infections and disease research
- Helminth infection and control
- Monoclonal and Polyclonal Antibodies Research
Washington University in St. Louis
2023-2024
University of Pennsylvania
2021-2022
Children's Hospital of Philadelphia
2021-2022
Duke University
2017-2020
Duke Medical Center
2017
Duke University Hospital
2017
University of Delaware
2015
Highlights•CRISPR activation used in a genome-wide screen for viral restriction factors•B4GALNT2 was the major hit screen, and restricted IAV infection•B4GALNT2 modifies glycans containing α2,3-linked sialic acids•Glycan modification by B4GALNT2 infection all tested avian strainsSummaryInfluenza A virus (IAV) is pathogen that poses significant risks to human health. It therefore critical develop strategies prevent influenza disease. Many loss-of-function screens have been performed identify...
Influenza virus vaccine production is currently limited by the ability to grow circulating human strains in chicken eggs or cell culture. To facilitate cost-effective growth, are serially passaged under conditions, which frequently results mutations of major antigenic protein, viral hemagglutinin (HA). Human vaccination with an antigenically drifted strain known contribute poor efficacy. address this problem, we developed a replication-competent influenza A (IAV) artificial genomic...
A range of chemical strategies have been used for crosslinking recombinant polypeptide hydrogels, although only a few employed photocrosslinking approaches. Here, we capitalize on the novel insect protein, resilin, and versatility click reactions to introduce resilin-like (RLP) that is capable photoinitiated thiol-ene crosslinking. Lysine residues RLP were functionalized with norbornene acid as confirmed via 1H-NMR spectroscopy. The RLPNs subsequently photocrosslinked multi-arm PEG thiols in...
Influenza B virus (IBV) is an acute, respiratory RNA that has been assumed to induce the eventual death of all infected cells. We and others have shown however, infection with apparently cytopathic viruses does not necessarily lead cell death; some cells can intrinsically clear persist in host long-term. To determine if any survive direct IBV infection, we here generate a recombinant capable activating host-cell reporter permanently label Using this system, demonstrate leads formation...
Although extended-spectrum cephalosporin-resistant Enterobacterales (ESCrE) and carbapenem-resistant (CRE) are a global challenge, data on these organisms in low- middle-income countries limited. In this study, we sought to characterize colonization critical for greater antibiotic resistance surveillance efforts.This study was conducted three hospitals six clinics Botswana. We ongoing of adult patients adults children the community. All participants underwent rectal swab sampling identify...
The BioFire FilmArray Pneumonia Panel (BFPP), a multiplex PCR panel for the diagnosis of lower respiratory tract infections, has been proposed as tool antimicrobial stewardship. Few studies evaluate real-world implementation BFPP and no focus exclusively on children. Our institution implemented testing without restrictions. We conducted retrospective cohort study in children hospitalized at St. Louis Children's Hospital to (1) characterize use pediatric patients (2) assess how results...
Influenza A virus (IAV) is a seasonal pathogen with the potential to cause devastating pandemics. IAV infects multiple epithelial cell subsets in respiratory tract, eliciting damage lungs. Clearance of primarily dependent on CD8+ T cells, which must balance control infection immunopathology. Using expressing Cre recombinase permanently label infected cells Cre-inducible reporter mouse, we previously discovered club that survive both lytic replication and cell-mediated clearance. In this...
Abstract Objectives Broad-range bacterial polymerase chain reaction with sequencing (BRBPS) provides valuable diagnostic data, especially in cases of culture-negative infections. However, as BRBPS testing demonstrates generally low positivity, cost per impactful result can be high and commonly involves longer turnaround times compared other methods, targeting use this assay to high-yield situations remains a challenging goal. Diagnostic stewardship help alleviate these challenges increase...
Influenza viruses initiate infection in the upper respiratory tract (URT), but early viral tropism and importance of cell-type-specific antiviral responses this tissue remain incompletely understood. By infecting transgenic lox-stop-lox reporter mice with a Cre-recombinase-expressing influenza B virus, we identify olfactory sensory neurons (OSNs) as major cell target URT. These cells become infected, then eliminate virus survive host post-resolution infection. OSN to are characterized by...
Abstract Infectious diseases account for significant morbidity and mortality in healthcare, metagenomic next-generation sequencing of plasma microbial cell-free DNA has emerged as a promising non-invasive diagnostic tool this scenario. However, the available data on real impact test clinical practice is limited conflicting. In context, we performed retrospective evaluation utilization at our institution since February 17, 2019. A total 76 tests were period 68 different patients. The mean age...
Abstract Background This study outlines the development, implementation, and impact of a laboratory-developed, extraction-free real-time PCR assay as primary diagnostic test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in pediatric hospital. Methods Clinical specimens from both upper lower tract sources were validated, including nasopharyngeal aspirates, swabs, anterior nares tracheal aspirates (n = 333 clinical samples). Testing volumes laboratory turnaround times then...
A 79-year-old man with a medical history of hypertension presented to the hospital acute-on-chronic back pain. Ten weeks prior presentation, he developed severe, persistent lower pain while working on construction project, which progressed until required cane and ultimately walker for mobility. He had previously been active projects, biking, hiking. Two was evaluated by neurosurgeon, magnetic resonance imaging (MRI) revealed L4-L5 disc enhancement, concerning discitis. An outpatient...
Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription (RT-PCR) a transparent accessible qualitative method for SARS-CoV-2 detection from nasopharyngeal oral pharyngeal samples with potential generate actionable data more quickly, at lower cost, fewer experimental resources than full RT-qPCR. This study...
Nucleic acid amplification tests (NAATs) are frequently used in
Abstract Reverse transcription–quantitative polymerase chain reaction (RT-qPCR) is used worldwide to test and trace the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). “Extraction-less” or “direct” real time–reverse transcription (RT-PCR) an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal oral pharyngeal samples with potential generate actionable data more quickly, at a lower cost, fewer experimental resources than full RT-qPCR. This study...