A5014619518- Neuroscience of respiration and sleep
- Hormonal Regulation and Hypertension
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Adipose Tissue and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Dental Anxiety and Anesthesia Techniques
- Inflammatory mediators and NSAID effects
- Neuropeptides and Animal Physiology
- Cannabis and Cannabinoid Research
- Oral microbiology and periodontitis research
- Nitric Oxide and Endothelin Effects
- Birth, Development, and Health
- Cancer, Hypoxia, and Metabolism
- Phosphodiesterase function and regulation
- Lipid metabolism and disorders
- Cleft Lip and Palate Research
- Neuroendocrine regulation and behavior
- Salivary Gland Disorders and Functions
- Sodium Intake and Health
- Peroxisome Proliferator-Activated Receptors
- Cancer, Stress, Anesthesia, and Immune Response
- Apelin-related biomedical research
- Analytical Methods in Pharmaceuticals
- Angiogenesis and VEGF in Cancer
Medical College of Wisconsin
2002-2024
Rzeszów School of Engineering and Economics
2021
Southern Illinois University School of Medicine
2016-2017
Universidade de São Paulo
2010-2017
Milwaukee VA Medical Center
2002-2005
University of Iowa
1996
Carotid body denervation (CBD) in neonatal goats and piglets results minimal irregular breathing no fatalities. Redundancy and/or plasticity of peripheral chemosensitivity a relatively mature ventilatory control system at birth may contribute to the paucity CBD effects these species. In present study, we tested hypothesis that mortality would be greater neonates less species such as rat. We found rats denervated 2-3 7-8 days age was significantly higher (P < 0.05) than sham-CBD rats. all...
Ventilatory sensitivity to CO(2) in awake adult Brown Norway (BN) rats is 50-75% lower than Sprague-Dawley (SD) and salt-sensitive Dahl S (SS) rats. The purpose of the present study was test hypothesis that this difference would be apparent during development sensitivity. Four litters each strain were divided into four groups such exposed 7% inspired for 5 min a plethysmograph every third day from postnatal (P) 0 P21 again on P29 P30. From P0 P14, exposure increased pulmonary ventilation...
Background: Fibroblasts are now seen as active components of the immune response because these cells express Toll‐like receptors (TLRs), recognize pathogen‐associated molecular patterns, and mediate production cytokines chemokines during inflammation. The innate host to lipopolysaccharide (LPS) from Porphyromonas gingivalis is unusual inasmuch different studies have reported that it can be an agonist for receptor 2 (TLR2) antagonist or 4 (TLR4). This study investigates compares whether...
The aim of this study was to quantify and compare the production transforming growth factor beta (TGF-β), interleukin (IL)-8 IL-10 by human cultured periodontal ligament gingival fibroblasts both obtained from same donors challenged with lipopolysaccharide (LPS) Porphyromonas gingivalis. Fibroblasts were exposed 0.1-10 µg/mL LPS P. gingivalis after 24 h supernatants collected analyzed enzyme-linked immunosorbent assay (ELISA). TGF-β protein upregulated in a concentration-dependent manner,...
Our aim was to determine the effects of focal acidification in raphe obscurus (RO) and pallidus (RP) on ventilation other physiological variables both awake sleep states adult goats. Through chronically implanted microtubules, 1) a acidosis created by microdialysis mock cerebrospinal fluid (mCSF), equilibrated with various levels CO2, 2) medullary extracellular (ECF) pH measured using custom-made electrode. Focal RO or RP, dialyzing either 25 80% CO2 (mCSF approximately 6.8 6.3), increased...
OBJECTIVE: This study investigated the role of periodontal disease in development stroke or cerebral infarction patients by evaluating clinical conditions and subgingival levels periodontopathogens. MATERIAL AND METHODS: Twenty with ischemic (I-CVA) hemorrhagic (H-CVA) cerebrovascular episodes (test group) 60 systemically healthy (control were evaluated for: probing depth, attachment level, bleeding on plaque index. Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans both...
This double-blind crossover randomized clinical trial compared the efficacy of 2 concentrations articaine, 2% (A2) and 4% (A4), with 1:200,000 epinephrine, for lower third molar removal. During separate appointments either A2 or A4, both similarly positioned molars in 46 volunteers were extracted. The following evaluated: onset duration anesthetic action on soft tissues, intraoperative bleeding, hemodynamic parameters, postoperative analgesia, mouth opening wound healing during 7th day,...
This study aimed to compare the quantity and quality of human DNA extracted from saliva that was fresh or frozen for three, six twelve months using five different extraction protocols: protocol 1 - Oragene™ commercial kit, 2 QIAamp mini 3 ammonium acetate, 4 Instagene™ Matrix 5 diluted 1:1 proteinase K 1% SDS. Briefly, analyzed spectrophotometry, electrophoresis PCR.Results indicated time spent in storage typically decreased with exception 1. The purity generally not affected by times based...
This study investigated whether ACTN3 R577X, AMPD1 C34T, I/D ACE, and M235T AGT polymorphisms can affect performance tests such as jumping, sprinting, endurance in 220 young male athletes from professional minor league soccer team São Paulo Futebol Clube, Brazil. ACE were also analyzed according to cardiac hemodynamic parameters. Athletes grouped or not by age. DNA saliva Taqman assays used for genotyping the results associated with tests. Ventricle mass, ventricle end-diastolic diameter,...
In an effort to contribute proper dental planning and define possible phenotypes of nonsyndromic cleft lip and/or palate (CL/P), this study aimed investigate the occurrence taurodontism, root dilaceration, tooth transposition in persons with CL/P, specifically analyzing differences among gender, types, most affected teeth.This retrospective analyzed 974 panoramic x-rays from Brazilians older than 16 years categorized into following four groups: group 1, 250 unilateral palate; 2, lip; 3, 224...
This study investigated how proteins of the insulin signaling cascade could modulate resistance after dexamethasone (Dexa) treatment and aerobic training. Rats were distributed into 4 groups: sedentary control (SC), sedentary+Dexa (SD), trained (TC), trained+Dexa (TD), underwent training for 70 days or remained sedentary. Dexa was administered during last 10 (1 mg · kg<sup>−1</sup> per day i. p.). After days, an intraperitoneal glucose tolerance test (ipGTT) performed. Protein levels IRS-1,...
Objective: Nonsteroidal anti-inflammatory drugs (NSAIDs) are metabolized by the cytochrome P450 enzymes (CYPs), predominantly CYP2C8 and CYP2C9. The aim of this study was to evaluate possible association polymorphisms in * 3 CYP2C9 genes with clinical efficacy oral piroxicam (20 mg daily for 4 days) after lower third molar surgeries regard postoperative pain, swelling, trismus, adverse reactions, need rescue medication volunteer's overall satisfaction. Materials methods: For purpose, 102...
Arterial stiffness predicts cardiovascular disease and all-cause mortality, but its treatment remains challenging. Mice treated with angiotensin II (Ang II) develop hypertension, arterial stiffness, vascular dysfunction, a downregulation of Rho-related BTB domain-containing protein 1 (RhoBTB1) in the vasculature. RhoBTB1 is associated blood pressure regulation, function poorly understood. We tested hypothesis that restoring can attenuate dysfunction Ang II-treated mice. Genetic...
GPCRs (G protein-coupled receptors) are implicated in blood pressure (BP) and fluid intake regulation. There is a developing concept that these effects mediated by both canonical G protein signaling noncanonical β-arrestin signaling, but the contributions of each remain largely unexplored. Here, we hypothesized contributes to homeostasis regulation deoxycorticosterone acetate (DOCA) salt hypertension, prototypical model salt-sensitive hypertension.Global β-arrestin1 (Arrb1) β-arrestin2...
The objective of the present study was to test hypothesis that in neonatal piglets there would be no hypoventilation after sham denervation or aortic (AOD) alone, but transient carotid body (CBD) and greatest combined (CBD+AOD). There a significant ( P < 0.05) CBD CBD+AOD denervated at 5, 15, 25 days age. 5 age greater than all other groups. Conversely, sham-denervated AOD did not hypoventilate denervation. Injections sodium cyanide showed chemoreceptors were site recovery peripheral...
Abstract Human hypertension caused by in-frame deletion of CULLIN3 exon-9 (Cul3∆9) is driven renal and vascular mechanisms. We bred conditionally activatable Cul3∆9 transgenic mice with tamoxifen-inducible Tie2-CREERT2 to test the importance endothelial Cul3. The resultant (E-Cul3∆9) trended towards elevated nighttime blood pressure (BP) correlated increased activity, but displayed no difference in daytime BP or activity. Male female E-Cul3∆9 together exhibited a decline...
Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used by the general population to alleviate inflammation and pain after oral surgeries. Piroxicam is among most commonly NSAIDs excels in controlling pain, swelling, trismus other common symptoms of inflammation. This study aimed evaluate different concentrations piroxicam its major metabolite, 5'-hydroxypiroxicam, human plasma samples over time using high performance liquid chromatography (HPLC) liquid-liquid extraction. Briefly, 10...
A digital rat brain atlas, in PDF format, was derived from high-resolution situ magnetic resonance images (MRI). The depict transverse, sagittal, and horizontal views contiguous 0.2 mm slices, with a planar resolution of 50 µm. Each slice indexed to the skull surface as well landmarks Bregma Lambda. Using common interactive file format enables users customize for their application, including image reproduction or without background labels. objective provide an open access supplementary guide...