A5016264173- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Metabolomics and Mass Spectrometry Studies
- Pregnancy and preeclampsia studies
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Gestational Diabetes Research and Management
- Mass Spectrometry Techniques and Applications
- Muscle metabolism and nutrition
- Cancer, Hypoxia, and Metabolism
- Sports Performance and Training
- Metabolism and Genetic Disorders
- Neonatal Health and Biochemistry
- Analytical Chemistry and Chromatography
- Fatty Acid Research and Health
University of Minnesota
2020-2025
University of Minnesota Medical Center
2025
University of Minnesota System
2024
Institute of Molecular Medicine
2022-2023
Ketogenesis is a dynamic metabolic conduit supporting hepatic fat oxidation particularly when carbohydrates are in short supply. Ketone bodies may be recycled into anabolic substrates, but physiological role for this process has not been identified. Here, we use mass spectrometry–based 13 C-isotope tracing and shotgun lipidomics to establish link between ketogenesis lipid anabolism. Unexpectedly, mouse liver primary hepatocytes consumed ketone support fatty acid biosynthesis via both de novo...
Throughout the last decade, interest has intensified in intermittent fasting, ketogenic diets, and exogenous ketone therapies as prospective health-promoting, therapeutic, performance-enhancing agents. However, regulatory roles of ketogenesis metabolism on liver homeostasis remain unclear. Therefore, we sought to develop a better understanding metabolic consequences hepatic body by focusing redox-dependent interconversion acetoacetate (AcAc) D-β-hydroxybutyrate (D-βOHB). Using targeted...
Background. Responses of the metabolome to acute aerobic exercise may predict maximum oxygen consumption (VO2max) and longer-term outcomes, including development diabetes its complications.
Metabolic reprogramming is a hallmark of cancer, enabling tumor cells to adapt and exploit their microenvironment for sustained growth. The liver common site metastasis, but the interactions between hepatocytes remain poorly understood. In context these play crucial role in promoting survival progression. This study leverages multiomics coverage via liquid chromatography high-resolution, high-mass-accuracy mass spectrometry-based untargeted metabolomics, 13C-stable isotope tracing, RNA...
The progression of metabolic dysfunction-associated steatotic liver disease (MASLD) to steatohepatitis (MASH) involves alterations in both liver-autonomous and systemic metabolism that influence the liver's balance fat accretion disposal. Here, we quantify contributions hepatic oxidative pathways injury MASLD-MASH. Using NMR spectroscopy, UHPLC-MS, GC-MS, performed stable-isotope tracing formal flux modeling fluxes humans across spectrum MASLD-MASH, mouse models impaired ketogenesis. In with...
Fatty acid esters of hydroxy fatty (FAHFA) are anti-diabetic and anti-inflammatory lipokines. Recently FAHFAs were also found to predict cardiorespiratory fitness in a cross-sectional study recreationally trained runners. Here we report the influences body composition gender on static FAHFA abundances circulation. We compared association between circulating concentrations composition, determined by dual x-ray absorptiometry, female recreational runners who lean (BMI < 25 kg/m 2 , n = 6),...
FA esters of hydroxy FAs (FAHFAs) are lipokines with extensive structural and regional isomeric diversity that impact multiple physiological functions, including insulin sensitivity glucose homeostasis. Because their low molar abundance, FAHFAs typically quantified using highly sensitive LC-MS/MS methods. Numerous relevant MS databases house in silico-spectra allow identification speciation FAHFAs. These provisional chemical feature assignments provide a useful starting point but could lead...
Metformin is a widely prescribed medication whose mechanism of action not completely defined and role in gestational diabetes management remains controversial. In addition to increasing the risk fetal growth abnormalities preeclampsia, associated with placental development including impairments trophoblast differentiation. Given that metformin impacts cellular differentiation events other systems, we assessed metformin’s impact on metabolism Using established cell culture models...
Summary Therapeutic interventions targeting hepatic lipid metabolism in metabolic dysfunction-associated steatotic liver disease (MASLD) and steatohepatitis (MASH) remain elusive. Using mass spectrometry-based stable isotope tracing shotgun lipidomics, we established a novel link between ketogenesis MASLD pathophysiology. Our findings show that mouse primary hepatocytes consume ketone bodies to support fatty acid (FA) biosynthesis via both de novo lipogenesis (DNL) FA elongation. Analysis of...
The progression of metabolic-dysfunction-associated steatotic liver disease (MASLD) to steatohepatitis (MASH) involves complex alterations in both liver-autonomous and systemic metabolism that influence the liver's balance fat accretion disposal. Here, we quantify relative contribution hepatic oxidative pathways injury MASLD-MASH. Using NMR spectroscopy, UHPLC-MS, GC-MS, performed stable-isotope tracing formal flux modeling fluxes humans across spectrum MASLD-MASH, mouse models impaired...
Abstract Metabolic reprogramming is a hallmark of cancer, enabling tumor cells to adapt and exploit their microenvironment for sustained growth. The liver common site metastasis, but the interactions between hepatocytes remain poorly understood. In context these play crucial role in promoting survival progression. This study leverages multiomics coverage via liquid chromatography high-resolution, high-mass accuracy mass spectrometry-based untargeted metabolomics, 13 C-stable isotope tracing,...
Abstract Cytotrophoblasts fuse to form and renew syncytiotrophoblasts necessary maintain placental health throughout gestation. During cytotrophoblast syncytiotrophoblast differentiation, cells undergo regulated metabolic transcriptional reprogramming. Mitochondria play a critical role in differentiation events cellular systems, thus we hypothesized that mitochondrial metabolism played central trophoblast differentiation. In this work, employed static stable isotope tracing untargeted...
Abstract Metformin is a widely prescribed medication whose mechanism of action not completely defined and role in gestational diabetes management remains controversial. In addition to increasing risks fetal growth abnormalities preeclampsia, associated with placental development including impairments trophoblast differentiation. Given that metformin impacts cellular differentiation events other systems, we assessed metformin’s impact on metabolism Using established cell culture models...
Abstract Fatty acid esters of hydroxy fatty (FAHFA) are anti-diabetic and anti-inflammatory lipokines. Recently FAHFAs were also found to predict cardiorespiratory fitness in trained runners. Here we compared the association between circulating FAHFA baseline concentrations body composition, determined by dual x-ray absorptiometry, female runners who lean (BMI < 25 kg/m 2 , n = 6), those overweight ≥ 7). We male (n 8) same 6) runner group. Circulating increased females a manner that was...
Abstract Cytotrophoblasts fuse to form and renew syncytiotrophoblasts necessary maintain placental health throughout gestation. During cytotrophoblast syncytiotrophoblast differentiation, cells undergo regulated metabolic transcriptional reprogramming. Mitochondria play a critical role in differentiation events cellular systems, thus we hypothesized that mitochondrial metabolism played central trophoblast differentiation. In this work, employed static stable isotope tracing untargeted...