A5000213146- Adipose Tissue and Metabolism
- Cardiovascular and exercise physiology
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Cardiovascular Effects of Exercise
- Heart Rate Variability and Autonomic Control
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Spaceflight effects on biology
- Metabolism and Genetic Disorders
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Smoking Behavior and Cessation
- Biochemical and Molecular Research
- Cancer, Stress, Anesthesia, and Immune Response
- RNA regulation and disease
- Cardiovascular Disease and Adiposity
- Regulation of Appetite and Obesity
- Congenital heart defects research
- Neurological diseases and metabolism
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
University of Minnesota Medical Center
2025
University of Louisville
2018-2024
University of Minnesota System
2024
University of Minnesota
2023-2024
Baxter (United States)
2018
E-cigarette use has surged, but the long-term health effects remain unknown. aerosols containing nicotine and acrolein, a combustion e-cigarette byproduct, may impair cardiac electrophysiology through autonomic imbalance. Here we show in mouse electrocardiograms that acute inhalation of disturbs conduction, part parasympathetic modulation. We demonstrate that, similar to acrolein or combustible cigarette smoke, from solvents (vegetable glycerin propylene glycol) induce bradycardia,...
Ketogenesis is a dynamic metabolic conduit supporting hepatic fat oxidation particularly when carbohydrates are in short supply. Ketone bodies may be recycled into anabolic substrates, but physiological role for this process has not been identified. Here, we use mass spectrometry–based 13 C-isotope tracing and shotgun lipidomics to establish link between ketogenesis lipid anabolism. Unexpectedly, mouse liver primary hepatocytes consumed ketone support fatty acid biosynthesis via both de novo...
The goal of this study was to develop an atlas the metabolic, transcriptional, and proteomic changes that occur with pregnancy in maternal heart. Timed studies FVB/NJ mice revealed a significant increase heart size by day 8 (midpregnancy; MP), which sustained throughout rest term compared nonpregnant control mice. Cardiac hypertrophy myocyte cross-sectional area were highest 7 days after birth (postbirth; PB) associated increases end-diastolic end-systolic left ventricular volumes higher...
Previous studies indicate that mitochondria-localized lactate dehydrogenase (mLDH) might be a significant contributor to metabolism. In the heart, presence of mLDH could provide cardiac mitochondria with higher capacity generate reducing equivalents directly available for respiration, especially during exercise when circulating levels are high. The purpose this study was test hypothesis contributes striated muscle bioenergetic function. Mitochondria isolated from murine and skeletal lacked...
Although the structural and functional effects of exercise on heart are well established, metabolic changes that occur in during after remain unclear. In this study, we used metabolomics to assess time-dependent murine cardiac metabolome following 1 session treadmill exercise. After bout, also recorded blood lactate, glucose, ketone body levels measured mitochondrial respiration. both male female mice, moderate- high-intensity acutely increased lactate levels. sexes, low- moderate-intensity...
Glucose metabolism comprises numerous amphibolic metabolites that provide precursors for not only the synthesis of cellular building blocks but also ATP production. In this study, we tested how phosphofructokinase-1 (PFK1) activity controls fate glucose-derived carbon in murine hearts vivo. PFK1 was regulated by cardiac-specific overexpression kinase- or phosphatase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase transgenes mice (termed GlycoLo GlycoHi mice, respectively)....
Coenzyme A (CoA) is an essential cofactor required for intermediary metabolism. Perturbations in homeostasis of CoA have been implicated various pathologies; however, whether changed and the extent to which levels contribute ventricular function remodeling during pressure overload has not explored. In this study, we sought assess changes biosynthetic pathway determine impact limiting on cardiac function. We limited by deleting rate-limiting enzyme biosynthesis, pantothenate kinase 1 (
The goal of the present study was to characterize changes in mitochondrial respiration maternal heart during pregnancy and after birth. Timed studies were performed 12-wk-old female FVB/NJ mice, cardiac mitochondria isolated from following groups mice: nonpregnant (NP), midpregnancy (MP), late (LP), 1-wk postbirth (PB). Similar our previous studies, we observed increased size all stages (e.g., MP LP) PB) compared with NP mice. Differential gene protein expression analyses revealed several...
Summary Therapeutic interventions targeting hepatic lipid metabolism in metabolic dysfunction-associated steatotic liver disease (MASLD) and steatohepatitis (MASH) remain elusive. Using mass spectrometry-based stable isotope tracing shotgun lipidomics, we established a novel link between ketogenesis MASLD pathophysiology. Our findings show that mouse primary hepatocytes consume ketone bodies to support fatty acid (FA) biosynthesis via both de novo lipogenesis (DNL) FA elongation. Analysis of...
The progression of metabolic-dysfunction-associated steatotic liver disease (MASLD) to steatohepatitis (MASH) involves complex alterations in both liver-autonomous and systemic metabolism that influence the liver's balance fat accretion disposal. Here, we quantify relative contribution hepatic oxidative pathways injury MASLD-MASH. Using NMR spectroscopy, UHPLC-MS, GC-MS, performed stable-isotope tracing formal flux modeling fluxes humans across spectrum MASLD-MASH, mouse models impaired...
Cardiometabolic complications of obesity present a growing public health concern and are associated with poor outcomes, mediated in part by an increased risk for cardiovascular disease, metabolic dysfunction-associated fatty liver systemic insulin resistance. Recent studies support that both resistance also aberrant brain metabolism cognitive impairment similar to what is observed neurodegenerative diseases. Central these pathological outcomes adverse changes tissue glucose ketone body...
Background: Electronic cigarette (e-cig) use has mounted. Some health authorities have even proclaimed e-cigs are profoundly safer than conventional cigarettes. However, generate several potentially cardiotoxic constituents by heating their solvent vehicles propylene glycol (PG) and vegetable glycerin (VG) into aerosols to deliver nicotine flavors. Objectives: To investigate the effects of chronic E-cig exposures, with or without nicotine, on cardiac gene expression compared mainstream smoke...
Cardiovascular disease is the leading cause of death in pregnant and postpartum women. During pregnancy, maternal heart rapidly adapts to increasing physiological metabolic demands growing fetus. This adaptation often takes form a hypertrophy which grows increase cardiac output; however, molecular processes underlying pregnancy-induced (PIH) are poorly understood. The goal this study was examine transcriptomic signatures associated with structural functional adaptations pregnancy. Therefore,...
Energy provision and biosynthesis are thought to be controlled by amphibolic enzymes such as phosphofructokinase (PFK). Nevertheless, it remains unclear how PFK influences collateral biosynthetic pathways in the heart. Here, we investigated control exerted on anabolic heart using vivo deep network tracing. Wild-type (WT) mice overexpressing kinase- or phosphatase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase transgenes, termed respectively Glyco Lo Hi mice, were fed a liquid...