A5090359031- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Muscle Physiology and Disorders
- Gestational Diabetes Research and Management
- Cardiomyopathy and Myosin Studies
- Diet and metabolism studies
- Muscle metabolism and nutrition
- Regulation of Appetite and Obesity
- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Metabolism, Diabetes, and Cancer
- Sirtuins and Resveratrol in Medicine
- Heat shock proteins research
- Ergonomics and Musculoskeletal Disorders
- Sports Performance and Training
- Adipokines, Inflammation, and Metabolic Diseases
- Mesenchymal stem cell research
- Cardiovascular and exercise physiology
- Muscle activation and electromyography studies
- Pregnancy-related medical research
- Whipple's Disease and Interleukins
- Advanced Sensor and Energy Harvesting Materials
- Mitochondrial Function and Pathology
- Genetic and Kidney Cyst Diseases
- Endoplasmic Reticulum Stress and Disease
Duke University
2023-2024
East Carolina University
2018-2024
Duke Medical Center
2023-2024
Duke University Hospital
2023
Salisbury University
2015-2021
University of Illinois Chicago
2017
Two coding variants of apolipoprotein L1 (APOL1) called G1 and G2 explain much the excess risk kidney disease in African Americans. While various cytotoxic phenotypes have been reported experimental models, proximal mechanism by which cause is poorly understood. Here, we leveraged three models a recently small molecule blocker APOL1 protein, VX-147, to identify upstream G1-induced cytotoxicity. In HEK293 cells, demonstrated that G1-mediated Na+ import/K+ efflux triggered activation G...
Resveratrol, a natural polyphenol compound contained in numerous plants, has been proposed as treatment for obesity-related disease processes such insulin resistance. However, humans there are conflicting results concerning the efficacy of resveratrol improving action; purpose present study was to determine whether obesity status (lean, severely obese) affects response human skeletal muscle. Primary muscle cells were derived from biopsies obtained age-matched lean and insulin-resistant women...
Skeletal muscle insulin resistance is a hallmark of Type 2 diabetes (T2DM) and may be exacerbated by protein modifications methylglyoxal (MG), known as dicarbonyl stress. The glyoxalase enzyme system composed 1/2 (GLO1/GLO2) the natural defense against stress, yet its expression, activity, regulation remain largely unexplored in skeletal muscle. Therefore, this study investigated stress subjects with T2DM (age: 56 ± 5 yr.; BMI: 32 kg/m ) compared lean healthy control (LHC; age: 27 1 22 )....
Exercise/exercise training can enhance insulin sensitivity through adaptations in skeletal muscle, the primary site of insulin-mediated glucose disposal; however, humans range improvement vary substantially. The purpose this study was to determine if obesity influences magnitude exercise response relation improving human muscle. Electrical pulse stimulation (EPS; 24 h) muscle myotubes improved action tissue from both lean and severely obese individuals, but responses EPS were blunted with...
Abstract Context Recent preclinical data suggest exercise during pregnancy can improve the metabolic phenotype not only of mother, but developing offspring as well. However, investigations in human are lacking. Objective To characterize effect maternal aerobic on offspring’s mesenchymal stem cells (MSCs). Design Randomized controlled trial. Setting Clinical research facility. Patients Healthy female adults between 18 and 35 years age ≤ 16 weeks’ gestation. Intervention Mothers were...
This study is the first to investigate effects of aerobic exercise intensity on circulating sRAGE isoforms, muscle RAGE protein, and splicing. isoforms tended diminish with exercise, although this effect was attenuated increasing intensity. Muscle protein gene expression were unaffected by exercise. However, individuals obesity displayed nearly twofold higher expression, which positively correlated P65 subunit NF-κB.
The progression of metabolic-dysfunction-associated steatotic liver disease (MASLD) to steatohepatitis (MASH) involves complex alterations in both liver-autonomous and systemic metabolism that influence the liver's balance fat accretion disposal. Here, we quantify relative contribution hepatic oxidative pathways injury MASLD-MASH. Using NMR spectroscopy, UHPLC-MS, GC-MS, performed stable-isotope tracing formal flux modeling fluxes humans across spectrum MASLD-MASH, mouse models impaired...
Thioredoxin-interacting protein (TXNIP) negatively effects the redox state and growth signaling via its interactions with thioredoxin (TRX) regulated in development DNA damage response 1 (REDD1), respectively. TXNIP expression is downregulated by pathways activated during aerobic exercise (AE), posttranslational modifications (PTMs; serine phosphorylation ubiquitination). The purpose of this investigation was to determine acute AE on expression, modifications, interacting partners, REDD1...
Preclinical rodent and nonhuman primate models investigating maternal obesity have highlighted the importance of intrauterine environment in development insulin resistance offspring; however, it remains unclear if these findings can be translated to humans. To investigate possible effects humans, we isolated mesenchymal stem cells (MSCs) from umbilical cord tissue infants born mothers normal weight or with obesity. Insulin-stimulated glycogen storage was determined MSCs undergoing myogenesis...
Abstract Background Optimal maternal metabolism during pregnancy is essential for healthy fetal growth and development. Chronic exercise shown to positively affect metabolism, predominantly demonstrated in nonpregnant populations. Objective To determine the effects of aerobic on metabolic biomarkers pregnancy, with expected lower levels glucose, insulin, lipids among exercise‐trained pregnant women. Methods Secondary data analyses were performed using from two, longitudinal prenatal...
Recent evidence identifies a potent role for aerobic exercise to modulate the activity of hypothalamic neurons related appetite; however, these studies have been primarily performed in male rodents. Since females markedly different neuronal mechanisms regulating food intake, current study aimed determine effects acute treadmill on neuron populations involved appetite female mice. Mature, untrained mice were exposed sedentary, low- (10 m/min), moderate- (14 and high (18 m/min)-intensity...
Emerging evidence identifies a potent role for aerobic exercise to modulate activity of neurons involved in regulating appetite; however, these studies produce conflicting results. These discrepancies may be, part, due methodological differences, including differences intensity and pre-exercise energy status. Consequently, the current study utilized translational, well-controlled, within-subject, treadmill protocol investigate differential effects status on post-exercise feeding behavior...
BACKGROUND: Resistance exercise provides an effective stimulus for improving the metabolic plasticity of skeletal muscle, and type acute muscle contraction plays important role in determining specific responses adaptations. The purpose current investigation was to examine effect order on by comparing monophasic concentric eccentric squats versus a protocol incorporating alternated repetitions.METHODS: Twelve recreationally active men (21.1±1.1yr) performed three nearly identical squat...
PURPOSE: The purpose of this study was to examine the validity Tendo Weightlifting Analyzer versus an infrared photocell laser timing system for assessing average power. A 1 kg-mass fixed dropped various distances ranging from 0.309 0.539m. METHODS: used corresponded barbell displacements subjects in a related who performed four different bench press trials using load and contraction intensities. As result there were per subject, resulting 36 (some subjects’ identical by chance). In we 4...
PURPOSE: Previous research has reported valid (within 15%) measurement of power during, bench press exercise using Tendo weightlifting analyzers. However, the accuracy measurements not been evaluated when different concentric contractions were used. The purpose this study was to examine comparability Tendo™ Sports Weightlifting Analyzer during recreational and explosive contractions. METHODS: 12 college-aged males with moderate experience performed 1RM press, two familiarizations, 4...
Resveratrol is a polyphenol compound that has been used for the prevention and treatment of obesity-related diseases. Precisely, some studies have indicated resveratrol improves insulin sensitivity in vivo, but these results are inconsistent yet to include severely obese cohort (BMI > 40 kg/m2). Preliminary research indicates myotubes derived from individuals resistant sensitizing effects resveratrol, mechanism remains undefined. PURPOSE: To determine on basal insulin-mediated glucose...
Introduction Exercise training has been shown to be effective in improving skeletal muscle insulin sensitivity, but individuals with severe obesity and/or type 2 diabetes (T2D) may not respond as robustly lean individuals. Electrical pulse stimulation (EPS) applied primary human cell cultures (myotubes) used an vitro exercise model understand responses contractile activity alone. Purpose The purpose of this study was examine the effect antecedent EPS on signaling myotubes from and severely...