Alzheimer’s disease is a neurodegenerative disorder that affects the central nervous system. In this study, we characterized and examined early metabolic changes in triple transgenic mouse AD model (3xtg-AD), their relationship with hypothalamus, key regulator of metabolism We observed 3xtg-AD exhibited significantly higher oxygen consumption as well food intake before reported amyloid plaque formation, indicating abnormalities occurred at onset compared counterparts. Analysis gene...

α-Klotho, a known anti-aging protein, exerts diverse physiological effects including: maintenance of phosphate and calcium homeostasis, modulation cell proliferation, enhanced buffering reactive oxygen species. However, the role α-Klotho in regulation energy metabolism is complex poorly understood. Here we investigated 5 weeks peripheral administration high fat diet induced obese mice. Food intake, blood glucose, body weight were measured daily. Energy expenditure was determined with...

Our laboratory recently identified the centrally circulating α-klotho protein as a novel hypothalamic regulator of food intake and glucose metabolism in mice. The current study aimed to investigate molecular effectors central arcuate nucleus hypothalamus (ARC), while further deciphering its role regulating energy balance both humans Cerebrospinal fluid (CSF) was collected from 22 adults undergoing lower limb orthopedic surgeries, correlations between body weight were determined using an...

While much is known about the role of agouti-regulated peptide/neuropeptide Y (AgRP/NPY) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little how forced expenditure, such as exercise, modulates these if are involved in post-exercise feeding behaviors. We utilized multiple mouse models investigate effects acute, moderate-intensity exercise on food intake neuronal activity arcuate nucleus (ARC) hypothalamus. NPY-GFP reporter mice were for immunohistochemistry...

α-Klotho is a circulating factor with well-documented antiaging properties. However, the central role of α-klotho in metabolism remains largely unexplored. The current study investigated potential to modulate neuropeptide Y/agouti-related peptide (NPY/AgRP)-expressing neurons, energy balance, and glucose homeostasis. Intracerebroventricular administration suppressed food intake, improved profiles, reduced body weight mouse models type 1 2 diabetes. Furthermore, inhibition via an...

Abstract A central question in the field of aging research is to identify cellular and molecular basis neuroresilience. One potential candidate small GTPase, Rab10. Here, we used Rab10 +/− mice investigate mechanisms underlying Rab10-mediated Brain expression analysis 880 genes involved neurodegeneration showed that have increased activation pathways associated with neuronal metabolism, structural integrity, neurotransmission, neuroplasticity compared their +/+ littermates. Lower was...

Vertical sleeve gastrectomy (VSG) is an effective surgery to treat obesity and diabetes. However, the direct effect of VSG on metabolic functions not fully understood. We aimed investigate if alterations in hypothalamic neurons were linked with perturbations liver metabolism after energy intake-controlled obese mouse model. C57BL/6 hrNPY-GFP reporter mice received HFD for 12 weeks then divided into three groups: Sham (ad lib), sham (pair-fed) VSG, VSG. Food intake was measured daily, blood...

The potential to control feeding behavior via hypothalamic AgRP/NPY neurons has led many approaches modulate their excitability – particularly by glutamatergic input. In the present study using NPY-hrGFP reporter mice, we visualize neuronal metabotropic glutamate receptor 1 (mGluR1) expression and test effect of fasting on mGluR1 function. Using pharmacological agonist dihydroxyphenylglycine (DHPG) demonstrate enhanced capacity drive firing after overnight fasting, while antagonist 3-MATIDA...

Recent evidence identifies a potent role for aerobic exercise to modulate the activity of hypothalamic neurons related appetite; however, these studies have been primarily performed in male rodents. Since females markedly different neuronal mechanisms regulating food intake, current study aimed determine effects acute treadmill on neuron populations involved appetite female mice. Mature, untrained mice were exposed sedentary, low- (10 m/min), moderate- (14 and high (18 m/min)-intensity...

Emerging evidence identifies a potent role for aerobic exercise to modulate activity of neurons involved in regulating appetite; however, these studies produce conflicting results. These discrepancies may be, part, due methodological differences, including differences intensity and pre-exercise energy status. Consequently, the current study utilized translational, well-controlled, within-subject, treadmill protocol investigate differential effects status on post-exercise feeding behavior...

This study investigated the effects of coffee ingestion with supplemental caffeine (CAF) on serum testosterone (T) responses to exercise in recreationally strength-trained males.Subjects ingested 6 mg/kg body weight via 12 ounces supplemented anhydrous or decaffeinated (DEC) prior a randomized, within-subject, crossover design. The session consisted 21 minutes high-intensity interval cycling (alternating intensities at power outputs associated 2.0 mmol/L lactate for two and 4.0 one minute)...

Premedication with acetaminophen and/or diphenhydramine to prevent febrile nonhemolytic transfusion reactions and minor allergic is a common practice based on historical recommendations. However, recent small randomized-controlled trials showed no benefit of premedication. This inconsistency leads variability, which results in the inefficiency our institution's blood product ordering process. project aimed improve number encounters premedication plan documentation from baseline 19% 80% 12...

We present an elementary proof of a reduced version Gleason's theorem and the Kochen-Specker to provide novel perspective on relation between both theorems. The is based set linear equations for values function $m$ unit sphere. In case entire sphere needs be considered, while finite points suffices prove theorem.

Background/Aims: α-Klotho is a circulating factor with well-documented anti-aging properties; however, the central role of in metabolism remains largely unexplored. We aimed to investigate potential α-klotho as novel regulator energy balance and glucose homeostasis. Methods: Central administration was performed by using intracerebroventricular (ICV) injection for seven days diet-induced obesity (DIO) streptozotocin-injected (STZ) mice. hrNPY-GFP reporter mice were utilized electrophysiology...

α-Klotho is a circulating factor with well-documented anti-aging properties; however, the central role of α-klotho in metabolism remains largely unexplored. The current study investigated potential to modulate NPY/AgRP neurons, energy balance, and glucose homeostasis. Intracerebroventricular (ICV) administration suppressed food intake, improved profiles, reduced body weight mouse models Type I II diabetes. Furthermore, inhibition via an anti-α-klotho antibody impaired tolerance. <i>Ex...

α-Klotho is a circulating factor with well-documented anti-aging properties; however, the central role of α-klotho in metabolism remains largely unexplored. The current study investigated potential to modulate NPY/AgRP neurons, energy balance, and glucose homeostasis. Intracerebroventricular (ICV) administration suppressed food intake, improved profiles, reduced body weight mouse models Type I II diabetes. Furthermore, inhibition via an anti-α-klotho antibody impaired tolerance. <i>Ex...