A5040776413- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Fungal and yeast genetics research
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Algal biology and biofuel production
- Plant responses to water stress
- Diatoms and Algae Research
- Plant tissue culture and regeneration
- Biotin and Related Studies
- Plant Micronutrient Interactions and Effects
- Phytase and its Applications
- Plant Virus Research Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Sugarcane Cultivation and Processing
- Plant Parasitism and Resistance
- Biofuel production and bioconversion
- Advanced Topology and Set Theory
- Plant pathogens and resistance mechanisms
- Metabolism, Diabetes, and Cancer
- Nematode management and characterization studies
Wroclaw Medical University
2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2010-2019
Martin Luther University Halle-Wittenberg
1982-2011
Syngenta (United States)
2006
Max Planck Institute of Molecular Plant Physiology
2005
University of Iowa
2004
University of Southampton
2003
Salk Institute for Biological Studies
1986-1999
University of Cambridge
1999
Leibniz Institute of Plant Genetics and Crop Plant Research
1997
Macromolecular trafficking within the sieve element–companion cell complex, phloem unloading, and post-phloem transport were studied using jellyfish green fluorescent protein (GFP). The GFP gene was expressed in Arabidopsis tobacco under control of AtSUC2 promoter. In wild-type plants, this promoter regulates expression companion cell–specific sucrose–H+ symporter gene. Analyses promoter–GFP plants demonstrated that 27-kD can traffic through plasmodesmata from cells into elements migrate...
Abstract For more than 400 million years, plants have maintained a mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi. This evolutionary success can be traced to the role of these fungi in providing mineral nutrients, particularly phosphate. In return, photosynthates are given fungus, which support its obligate biotrophic lifestyle. Although mechanisms involved phosphate transfer been extensively studied, less is known about reciprocal carbon. Here, we present high-affinity...
Summary An important, most likely essential step for the long distance transport of sucrose in higher plants is energy‐dependent, uncoupler‐sensitive loading into phloem cells via a sucrose‐H + symporter. This paper describes functional expression Saccharomyces cerevisiae two cDNAs encoding energy‐dependent transporters from plasma membrane Arabidopsis thaliana, SUC1 and SUC2 . Yeast transformed with vectors allowing either or under control promoter yeast ATPase gene ( PMA1 ) sucrose, to...
Summary In order to understand sucrose transport in developing seeds of cereals at the molecular level, we cloned from a caryopses library two cDNAs encoding transporters, designated HvSUT1 and HvSUT2. Sucrose uptake activity was confirmed by heterologous expression yeast. Both transporter genes are expressed maternal as well filial tissues. series situ hybridizations analysed cell type‐specific seeds. is preferentially cells nucellar projection endospermal transfer layer, which represent...
Developing Arabidopsis (Arabidopsis thaliana) seeds and embryos represent a complex set of cell layers tissues that mediate the transport partitioning carbohydrates, amino acids, hormones, signaling molecules from terminal end funicular phloem to between these seed eventually growing embryo. This article provides detailed analysis symplastic domains cell-to-cell connectivity funiculus embryo, within embryo during its maturation. The movement green fluorescent protein or mobile nonmobile...
Abstract: Polyclonal antisera against a fusion protein of β‐galactosidase and the 20 C‐terminal amino acids Arabidopsis thaliana sucrose carrier AtSUC2 were used to determine cellular localization protein. Using fluorescence‐labelling on sections from different organs was immunolocalized exclusively in companion cells. The presented data indicate that phloem loading may be catalyzed by which transports into No evidence for participation second transporter AtSUC1 has been obtained.
Summary Transgenic Arabidopsis plants were constructed to express a range of GFP‐fusion proteins (36–67 kDa) under the companion cell (CC)‐specific AtSUC2 promoter. These used monitor trafficking these from CCs into sieve elements (SEs) and their subsequent translocation within out phloem. The results revealed large size exclusion limit (SEL) (>67 for plasmodesmata connecting SEs in loading Membrane‐anchored GFP‐fusions GFP variant targeted endoplasmic reticulum (ER) remained inside as...
To analyze sugar transport processes during seed development of fava bean, we cloned cDNAs encoding one sucrose and hexose transporter, designated VfSUT1 VfSTP1, respectively. uptake activity was confirmed after heterologous expression in yeast. Gene studied relation to development. Transcripts were detected both vegetative tissues. In the embryo, VfSTP1 mRNAs only epidermal cells, but a different temporal spatial pattern. mRNA accumulates midcotyledon stage cells covering mitotically active...
Abstract Powdery mildew fungi are biotrophic pathogens that form a complex interface, the haustorium, between host plant and parasite. The pathogen acts as an additional sink, competing with sinks, resulting in considerable modification of photoassimilate production partitioning within tissue. Here, we examine factors may contribute to these changes. We show for first time one interaction (Arabidopsis/Erysiphe cichoracearum) all following responses: Glc uptake tissues is enhanced after...
Summary The vacuolar membrane is involved in solute uptake into and release from the vacuole, which largest plant organelle. In addition to inorganic ions metabolites, large quantities of protons sugars are shuttled across this membrane. Current models suggest that proton gradient drives accumulation and/or sugars. Recent studies have associated AtSUC4 with Some members SUC family plasma proton/sucrose symporters. addition, sugar transporters TMT1 TMT2, localized membrane, been suggested...
A cDNA for the Arabidopsis STP4 gene (for sugar transport protein 4) was isolated, and properties of encoded were studied in Schizosaccharomyces pombe. The monosaccharide H+ symporter is composed 514 amino acids has a calculated molecular mass 57.1 kD. RNA gel blot analyses revealed that expressed primarily roots flowers Arabidopsis. This shown more detail with promoter-beta-glucuronidase (GUS) plants yielding strong STP4-driven GUS activity root tips anthers. Wounding transformed STP4-GUS...
Plant pathogenic fungi cause massive yield losses and affect both quality safety of food feed produced from infected plants. The main objective plant is to get access the organic carbon sources their carbon-autotrophic hosts. However, chemical nature source(s) mode uptake are largely unknown. Here, we present a novel, plasma membrane-localized sucrose transporter (Srt1) corn smut fungus Ustilago maydis its characterization as fungal virulence factor. Srt1 has an unusually high substrate...
We have characterized three different transcripts induced by fungal elicitor, wounding, or infection which encode apoproteins of cell wall hydroxyproline-rich glycoproteins involved in plant defense against infection. The proteins encoded two these contain a proline-rich domain involving tandem repetition the 16-amino-acid unit Tyr3-Lys-Ser-Pro4-Ser-Pro-Ser-Pro4. third transcript encodes protein with variant this 16-mer canonical repeat: Tyr3-His-Ser-Pro4-Lys-His-Ser-Pro4. Each is separate...
Summary The Arabidopsis AtSUC1 protein has previously been characterized as a plasma membrane H + ‐sucrose symporter. This paper describes the sites of gene expression and localization assigns specific functions to this sucrose transporter in anther development pollen tube growth. RNase protection assays revealed exclusively floral tissue, which was confirmed by analyses promoter‐β‐glucuronidase ( GUS ) plants. In situ hybridizations identified connective funiculi fully developed grains....
Abstract The Arabidopsis AtSUC3 gene encodes a sucrose (Suc) transporter that differs in size and intron number from all other Suc transport proteins. Each plant species analyzed so far possesses one of this special type, several functions have been discussed for these proteins, including the catalysis transmembrane transport, also sensing regulation transporters. Here, we show protein is localized sieve elements phloem not colocalized with companion cell-specific AtSUC2 loader. Even...