A5060653991- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Gene expression and cancer classification
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Cell Adhesion Molecules Research
- Molecular Biology Techniques and Applications
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- Plant tissue culture and regeneration
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Genetics, Bioinformatics, and Biomedical Research
- Genetic Mapping and Diversity in Plants and Animals
- Barrier Structure and Function Studies
- Complement system in diseases
- Genetics and Plant Breeding
- Reproductive System and Pregnancy
- Fungal Biology and Applications
- Bioenergy crop production and management
- Cancer, Lipids, and Metabolism
- Plant Virus Research Studies
- Wheat and Barley Genetics and Pathology
University of Nottingham
2016-2025
Australian Football League
2021
Loughborough University
2016-2017
Vlaams Instituut voor Biotechnologie
2005-2010
Ghent University
2005-2010
Max Planck Institute of Molecular Plant Physiology
2010
Laboratoire d'Écophysiologie Moléculaire des Plantes sous Stress Environnementaux
2010
Research International (United States)
2009
Weizmann Institute of Science
2009
Radboud University Nijmegen
2009
The plant hormone auxin regulates various developmental processes including root formation, vascular development, and gravitropism. Mutations within the AUX1 gene confer an auxin-resistant growth phenotype abolish gravitropic curvature. Polypeptide sequence similarity to amino acid permeases suggests that mediates transport of acid-like signaling molecule. Indole-3-acetic acid, major form in higher plants, is structurally similar tryptophan a likely substrate for product. cloned can restore...
Our aim was to generate and prove the concept of "smart" plants monitor plant phosphorus (P) status in Arabidopsis. Smart can be genetically engineered by transformation with a construct containing promoter gene up-regulated specifically P starvation an accessible tissue upstream marker such as beta-glucuronidase (GUS). First, using microarrays, we identified genes whose expression changed more than 2.5-fold shoots growing hydroponically when P, but not N or K, withheld from nutrient...
Abstract Together, auxin and cytokinin regulate many of the processes that are critical to plant growth, development, environmental responsiveness. We have previously shown exogenous regulates biosynthesis in Arabidopsis thaliana. In this work, we show that, conversely, application or induced ectopic leads a rapid increase young, developing root shoot tissues. also reducing endogenous levels, either through induction CYTOKININ OXIDASE expression mutation one more biosynthetic...
We previously showed that the N6-methyladenosine (m6A) mRNA methylase is essential during Arabidopsis thaliana embryonic development. also demonstrated this modification present at varying levels in all mature tissues. However, requirement for m6A plant was not tested. Here we show a 95% reduction later growth stages gives rise to plants with altered patterns and reduced apical dominance. The flowers of these commonly defects their floral organ number, size identity. global analysis gene...
Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes enable this activity remain largely unknown. Here we show BRs participate the regulation of freezing tolerance. BR signaling-defective mutants Arabidopsis thaliana were hypersensitive to before and after cold acclimation. The constitutive activation signaling, contrast, enhanced resistance. Evidence is provided BR-controlled basic helix-loop-helix transcription factor...
Abstract We have investigated the subcellular localization, domain topology, and amino acid residues that are critical for function of presumptive Arabidopsis thaliana auxin influx carrier AUX1. Biochemical fractionation experiments confocal studies using an N-terminal yellow fluorescent protein (YFP) fusion observed AUX1 colocalized with plasma membrane (PM) markers. Because its PM we were able to take advantage steep pH gradient exists across plant cell investigate topology YFP as a...
Arabidopsis, because of its diminutive size, small genome, and rapid life cycle, has become the premiere research organism in plant biology. The power Arabidopsis as a model system will even greater now that all five chromosomes have been sequenced. Before 1990, extensive
Recent research has highlighted the importance of auxin concentration gradients during plant development. Establishment these is believed to involve polar transport through specialized carrier proteins. We have used an experimental system, wood-forming tissue hybrid aspen, which allows tissue-specific expression analysis genes and quantification endogenous concentrations hormone. As part this study, we isolated putative genes, PttLAX1-PttLAX3 PttPIN1-PttPIN3, belonging AUX1-like family...
Whole-genome transcriptome profiling is revealing how biological systems are regulated at the transcriptional level. This study reports development of a robust method to profile and compare transcriptomes two nonmodel plant species, Thlaspi caerulescens, zinc (Zn) hyperaccumulator, arvense, nonhyperaccumulator, using Affymetrix Arabidopsis thaliana ATH1-121501 GeneChip arrays (Affymetrix, Santa Clara, CA, USA). Transcript abundance was quantified in shoots agar- compost-grown plants both...
Saponins, a group of glycosidic compounds present in several plant species, have aglycone moieties that are formed using triterpenoid or steroidal skeletons. In spite their importance as antimicrobial and possible benefits for human health, knowledge the genetic control saponin biosynthesis is still poorly understood. Medicago genus, hemolytic activity saponins related to nature moieties. We identified cytochrome P450 gene (CYP716A12) involved synthesis truncatula combined biochemical...
Abstract A major goal of the life sciences is to understand how molecular processes control phenotypes. Because understanding biological systems relies on work multiple laboratories, biologists implicitly assume that organisms with same genotype will display similar phenotypes when grown in comparable conditions. We investigated what extent this holds true for leaf growth variables and metabolite transcriptome profiles three Arabidopsis (Arabidopsis thaliana) genotypes 10 laboratories using...
Abstract Background Short oligonucleotide arrays for transcript profiling have been available several years. Generally, raw data from these are analysed with the aid of Microarray Analysis Suite or GeneChip Operating Software (MAS GCOS) Affymetrix. Recently, more methods to analyse become available. Ideally all should come up less same results. We set out evaluate different and include work on our own set, in order test which method gives most reliable Results Calculating gene expression 6...
Medicago truncatula contains a family of at least five genes related to AUX1 Arabidopsis thaliana (termed MtLAX for truncatula-like genes). The high sequence similarity between the encoded proteins and implies that encode auxin import carriers. are expressed in roots other organs, suggesting they play pleiotropic roles uptake. In primary roots, preferentially root tips, particularly provascular bundles caps. During lateral nodule development, primordia, cells were probably derived from...
Abstract As a genetic platform, tomato (Solanum lycopersicum) benefits from rich germplasm collections and ease of cultivation transformation that enable the analysis biological processes impossible to investigate in other model species. To facilitate assembly an open toolbox designed study Solanaceae, we initiated joint collection publicly available gene manipulation tools. We focused on characterization promoters expressed at defined time windows during fruit development, for regulated...
Interest in mRNA methylation has exploded recent years. The sudden interest a 40 year old discovery was due part to the finding of FTO's (Fat Mass Obesity) N6-methyl-adenosine (m6A) deaminase activity, thus suggesting link between obesity-associated diseases and presence m6A mRNA. Another catalyst rise research release methylomes for human, mouse Saccharomyces cerevisiae. However, molecular function, or functions this 'epimark' remain be discovered. There is supportive evidence that could...
<ns4:p>Artificial Intelligence (AI) is increasingly used within plant science, yet it far from being routinely and effectively implemented in this domain. Particularly relevant to the development of novel food agricultural technologies validated, meaningful usable ways integrate, compare visualise large, multi-dimensional datasets different sources scientific approaches. After a brief summary reasons for interest data science AI paper identifies discusses eight key challenges management that...
Abstract Transcript profiling is crucial to study biological systems, and various platforms have been implemented survey mRNAs at the genome scale. We assessed performance of CATMA microarray designed for Arabidopsis (Arabidopsis thaliana) transcriptome analysis compared it with Agilent Affymetrix commercial platforms. The array consists gene-specific sequence tags 150 500 bp, 2) 60mer oligonucleotides, gene chip (ATH1) 25mer oligonucleotide sets. matched each probe repertoire annotation...
The normal cellular counterpart of the v-fms oncogene product is a receptor for mononuclear phagocyte colony-stimulating factor, CSF-1. An interleukin-3 (IL-3)-dependent mouse myeloid cell line, FDC-P1, was infected with murine retrovirus vector containing linked to gene encoding resistance neomycin (neo). Infected cells selected aminoglycoside G418 contained few proviral DNA copies per haploid genome, expressed low levels v-fms-coded glycoprotein, remained IL-3 dependent growth, and were...
Abstract High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChip ® technology amongst the most widely used, although currently available only small number of plant and animal species. Thus, we have developed method to improve sensitivity high-density when applied heterologous species tested by analysing transcriptome Brassica oleracea L., which no array is available, using designed Arabidopsis thaliana (L.) Heynh. Genomic DNA from B....
Bambara groundnut (Vigna subterranea (L.) Verdc.) is an African legume and a promising underutilized crop with good seed nutritional values. Low temperature stress in number of countries at night, such as Botswana, can effect the growth development bambara groundnut, leading to losses potential yield. Therefore, this study we developed computational pipeline identify analyze genes gene modules associated low responses using cross-species microarray technique (as has no chip) coupled...
Plants are essential for human survival. Over the past three decades, work with reference plant Arabidopsis thaliana has significantly advanced biology research. One key event was sequencing of its genome 25 years ago, which fostered many subsequent research technologies and datasets. been instrumental in elucidating plant-specific aspects biology, developing tools, translating findings to crop improvement. It not only serves as a model understanding but also other fields, discoveries having...