A5005638913- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Algal biology and biofuel production
- Light effects on plants
- Neonatal Respiratory Health Research
- Protist diversity and phylogeny
- Plant biochemistry and biosynthesis
- Genetic and Environmental Crop Studies
- Lipid metabolism and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Plant Stress Responses and Tolerance
- Agricultural pest management studies
- Geology and Paleoclimatology Research
- Infant Nutrition and Health
- Pharmaceutical studies and practices
- Plant responses to water stress
- Antioxidant Activity and Oxidative Stress
- Plant Micronutrient Interactions and Effects
- Plant Diversity and Evolution
- Biocrusts and Microbial Ecology
- Paleontology and Stratigraphy of Fossils
University of Nottingham
2010-2023
University of Warwick
2017
Royal Holloway University of London
1997-2013
Nottingham Trent University
2013
Syngenta (United Kingdom)
2013
Syngenta (United States)
2013
University of London
2002
University of York
1985-1994
Ludwig Cancer Research
1983-1985
The Royal Melbourne Hospital
1982-1985
The Arabidopsis chlorophyll a/b binding protein (CAB) gene underexpressed 1 (cue1) mutant underexpresses light-regulated nuclear genes encoding chloroplast-localized proteins. cue1 also exhibits mesophyll-specific chloroplast and cellular defects, resulting in reticulate leaves. Both the underexpression leaf cell morphology phenotypes are dependent on light intensity. In this study, we determine that CUE1 encodes plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) define...
Plastids are an important group of plant cellular organelles and comprise one the primary features that distinguish cells from those other eukaryotes. thought to have arisen as a result endosymbiotic event in which early photosynthetic prokaryote invaded primitive
Abstract Replication of chloroplasts is essential for achieving and maintaining optimal plastid numbers in plant cells. The division machinery contains components both endosymbiotic host cell origin, but little known about the regulation molecular mechanisms that govern process. Arabidopsis mutant arc6 defective division, its leaf mesophyll cells contain only one or two grossly enlarged chloroplasts. We show here also exhibit abnormal localization key proteins FtsZ1 FtsZ2. Whereas wild-type...
Whole-genome transcriptome profiling is revealing how biological systems are regulated at the transcriptional level. This study reports development of a robust method to profile and compare transcriptomes two nonmodel plant species, Thlaspi caerulescens, zinc (Zn) hyperaccumulator, arvense, nonhyperaccumulator, using Affymetrix Arabidopsis thaliana ATH1-121501 GeneChip arrays (Affymetrix, Santa Clara, CA, USA). Transcript abundance was quantified in shoots agar- compost-grown plants both...
A nuclear recessive mutant of Arabidopsis thaliana, arc5, has been isolated in which there is no significant increase chloroplast number during leaf mesophyll cell expansion and are only 13 chloroplasts per compared with 121 wild-type cells. Mature arc5 fully expanded cells 6-fold larger than large proportion also show some degree central constriction, suggesting that the mutation prevented completion division process. To examine interaction arc loci, a double was constructed between arc1,...
Abstract Carotenoids represent some of the most important secondary metabolites in human diet, and tomato (Solanum lycopersicum) is a rich source these health-promoting compounds. In this work, novel fruit-related regulator pigment accumulation has been identified by artificial neural network inference analysis its function validated transgenic plants. A fruit gene regulatory was generated using transcription factor expression profiles derived from fruits sampled at various points during...
ABSTRACT Bundle sheath cells form a around the entire vascular tissue in Arabidopsis leaves and constitute distinct leaf cell type, as defined by their elongate morphology, position adjacent to vein differences chloroplast development compared mesophyll cells. They about 15% of chloroplast-containing leaf. In order identify genes which play role differential bundle chloroplasts, screen reticulate mutants was used new class termed dov (differential vascular-associated cells). The dov1 mutant...
We have isolated three mutants of Arabidopsis thaliana in which there is a sevenfold change chloroplast number fully expanded leaf mesophyll cells and increases decreases are compensated for by changes size. The stably inherited sexual crosses generations mutant phenotypes effected at single recessive nuclear loci, termed arc loci. This the first report large, higher plants, represents major advance toward genetic dissection control division.
Journal Article Temporal and Spatial Development of the Cells Expanding First Leaf Arabidopsis thaliana (L.) Heynh Get access K. A. PYKE, PYKE Department Biology, University YorkHeslington, York YOl 5DD, UK Search for other works by this author on: Oxford Academic PubMed Google Scholar J. L. MARRISON, MARRISON M. LEECH 1 1To whom correspondence should be addressed. Experimental Botany, Volume 42, Issue 11, November 1991, Pages 1407–1416, https://doi.org/10.1093/jxb/42.11.1407 Published: 01...
A novel mutant of Arabidopsis thaliana, arc6 (accumulation and replication chloroplasts), has been isolated from a transfer DNA-mutagenized population seedlings. the most extreme arc phenotype we have yet described, with only one to three chloroplasts per leaf mesophyll cell compared mean 83 in cells wild-type var Wassilewskija. The are 20-fold larger than chloroplasts.Chloroplast division is almost certainly precluded cells, since chloroplast number does not increase during expansion. long...
To analyze the genetic control of process chloroplast division, a direct image analysis screening procedure has been developed in which mutants Arabidopsis thaliana (L.) Heynh. var Landsberg erecta are selected on basis abnormal number. The selection is based thresholding after iodine staining, facilitates automatic counting chloroplasts isolated mesophyll cells. M2 seedlings screened for significant deviation from wild type relationship between cell size and Mutants with both abnormally...
An in vitro system has been developed to elucidate the nature of cellular defect primary immunodeficiency diseases. Incubation, on human thymic epithelial monolayer cultures, peripheral blood lymphocytes and bone-marrow cells from a child with documented severe combined disease resulted appearance population that formed rosettes sheep erythrocytes. The same cell preparation permitted synthesis antigen-specific, complement-dependent antibodies after education, as demonstrated plaque assay...
Abstract Imaging of chlorophyll autofluorescence by confocal microscopy in intact whole petals ofArabidopsis thaliana has been used to analyze chloroplast development and redifferentiation during petal development. Young dissected from unopened buds contained green chloroplasts throughout their structure, but as the upper part lamina developed expanded, plastids lost redifferentiated into leukoplasts, resulting a white blade. Normal remained stalk mature petal. In epidermal cells were normal...
Summary Stromules are motile extensions of the plastid envelope membrane, whose roles not fully understood. They present on all types but more common and extensive non‐green plastids that sparsely distributed within cell. During tomato fruit ripening, chloroplasts in mesocarp tissue differentiate into chromoplasts undergo major shifts morphology. In order to understand what factors regulate stromule formation, we analysed biogenesis tobacco hypocotyls two distinct populations mesocarp. We...
ABSTRACT The arc6 (accumulation and replication of chloroplasts) mutant Arabidopsis has only two greatly enlarged chloroplasts per mature leaf mesophyll cell compared with ninety in the wild type. mutation is a single nuclear gene plant phenotype normal. Shoot root apical meristems plants have been examined to determine how early during plastid development can be recognised. In cells meristem there are proplastids, which larger than type highly variable morphology. primordia where...
By using green fluorescent protein targeted to the plastid organelle in tomato (Lycopersicon esculentum Mill.), morphology of plastids and their associated stromules epidermal cells trichomes from stems petioles chromoplasts pericarp fruit has been revealed. A novel characteristic is presence extensive bead‐like structures along that are often observed as free vesicles, distinct apparently unconnected body. Interconnections between red pigmented chromoplast bodies common suggesting could...
The ability to measure cell proliferation is important in the study of cancer biology. usual technique for quantitating proliferating cells tissue explant and organ culture by detection [3H]-thymidine incorporation into DNA autoradiography tedious time-consuming. We have developed a identification quantitation bromodeoxyuridine (an analogue thymidine) cultured explants. Fetal mouse colon explants were exposed vitro (BUdR) or 3 72 hr then various periods unlabeled thymidine. tissues stained...
There is a need to develop rice plants with improved photosynthetic capacity and efficiency in order enhance potential grain yield. Alterations internal leaf morphology may be needed underpin some of these improvements. One target the production ‘Kranz-like’ anatomy, commonly considered required achieve desired levels photosynthesis seen C4 crops. Kranz anatomy typically has two or three mesophyll cells interspersing adjacent veins. As first step determining for such anatomical modifications...
The division of plastids is an important part plastid differentiation and development in distinct cell types, such as leaf mesophyll cells, results large populations chloroplasts. morphology population dynamics have been well documented, but the molecular controls underlying are largely unknown. With isolation Arabidopsis mutants which specific aspects proplastid disrupted, potential exists for a detailed knowledge how divide what factors control rate different types. It likely that plant...