A5072121280- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Fungal Biology and Applications
- Plant Growth Enhancement Techniques
- Plant Pathogens and Fungal Diseases
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Metal complexes synthesis and properties
- Plant Stress Responses and Tolerance
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Flowering Plant Growth and Cultivation
- Seed Germination and Physiology
- Plant Virus Research Studies
- Allelopathy and phytotoxic interactions
- Plant biochemistry and biosynthesis
- Plant Physiology and Cultivation Studies
- Banana Cultivation and Research
- Fungal Plant Pathogen Control
- Transgenic Plants and Applications
Czech Academy of Sciences, Institute of Experimental Botany
2016-2025
Palacký University Olomouc
2016-2025
Czech Academy of Sciences, Biology Centre
2017
Swedish University of Agricultural Sciences
1998-2015
Umeå Plant Science Centre
2003-2015
Czech Academy of Sciences
2014-2015
University of the Basque Country
2015
University of Padua
2010
University of Veterinary Sciences Brno
2010
Vlaams Instituut voor Biotechnologie
2008
One of the most long-lived models in plant science is belief that long-distance transport and ratio two hormones, auxin cytokinin, at site action control major developmental events such as apical dominance. We have used vivo deuterium labeling mass spectrometry to investigate dynamics homeostatic cross talk between hormones. Interestingly, mediates a very rapid negative cytokinin pool by mainly suppressing biosynthesis via isopentenyladenosine-5′-monophosphate-independent pathway. In...
Although a substantial proportion of plant biomass originates from the activity vascular cambium, molecular basis radial growth is still largely unknown. To address whether cytokinins are required for cambial activity, we studied cytokinin signaling across zones 2 tree species, poplar (Populus trichocarpa) and birch (Betula pendula). We observed an expression peak genes encoding receptors in dividing cells. reduced levels endogenously by engineering transgenic trees (P. tremula x...
Abstract Together, auxin and cytokinin regulate many of the processes that are critical to plant growth, development, environmental responsiveness. We have previously shown exogenous regulates biosynthesis in Arabidopsis thaliana. In this work, we show that, conversely, application or induced ectopic leads a rapid increase young, developing root shoot tissues. also reducing endogenous levels, either through induction CYTOKININ OXIDASE expression mutation one more biosynthetic...
We have developed a new analytical approach for isolation and quantification of cytokinins (CK) in minute amounts fresh plant material, which combines simple one-step purification with ultra-high performance liquid chromatography-fast scanning tandem mass spectrometry.Plant tissue samples (1-5 mg FW) were purified by stop-and-go-microextraction (StageTip purification), previously has only been applied clean-up pre-concentration peptides. found that combination two reverse phases one...
Plant cells are totipotent and competent to regenerate from differentiated organs. It has been shown that two phytohormones, auxin cytokinin, play critical roles within this process. As in animals, the regenerative capacity declines with age plants, but molecular basis for phenomenon remains elusive. Here, we demonstrate an age-regulated microRNA, miR156, regulates shoot capacity. a plant ages, gradual increase miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription...
Cytokinins (CKs) play a crucial role in many physiological and developmental processes at the levels of individual plant components (cells, tissues, organs) by coordinating activities across these parts. High-resolution measurements intracellular CKs different tissues can therefore provide insights into their metabolism mode action. Here, we applied fluorescence-activated cell sorting green fluorescent protein (GFP)-marked types, combined with solid-phase microextraction an...
Abstract It is known that volatile emissions from some beneficial rhizosphere microorganisms promote plant growth. Here we show compounds (VCs) emitted by phylogenetically diverse and non‐rhizhosphere bacteria fungi (including pathogens microbes do not normally interact mutualistically with plants) growth flowering of various species, including crops. In Arabidopsis plants exposed to VCs the phytopathogen Alternaria alternata , changes included enhancement photosynthesis accumulation high...
Abstract Plant hormone cytokinins are perceived by a subfamily of sensor histidine kinases (HKs), which via two-component phosphorelay cascade activate transcriptional responses in the nucleus. Subcellular localization receptors proposed endoplasmic reticulum (ER) membrane as principal cytokinin perception site, while study transport pointed to plasma (PM)-mediated signalling. Here, detailed monitoring subcellular localizations fluorescently labelled natural probe and receptor ARABIDOPSIS...
Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate zeatinriboside-5'-monophosphate was around 66-fold higher than isopentenyladenosine-5'-monophosphate (iPMP), proposed primary product Agrobacterium ipt. Double tracer analysis, using [(2)H(6)] isopentenyladenosine oxide, provided evidence for an alternative, iPMP-independent, pathway zeatin-type...
Abstract Lys-63–linked multiubiquitin chains play important roles in signal transduction yeast and mammals, but the functions for this type of chain plants remain to be defined. The RING domain protein RGLG2 (for Ligase2) from Arabidopsis thaliana can N-terminally myristoylated localizes plasma membrane. It form an vitro reaction. has overlapping with its closest sequelog, RGLG1, single mutants either gene are inconspicuous. rglg1 rglg2 double mutant exhibit loss apical dominance altered...