A5042183780- Photosynthetic Processes and Mechanisms
- Photodynamic Therapy Research Studies
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- Porphyrin and Phthalocyanine Chemistry
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Cancer-related Molecular Pathways
- Plant Molecular Biology Research
- Nanoplatforms for cancer theranostics
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Advanced Proteomics Techniques and Applications
- Plant Parasitism and Resistance
- Chromosomal and Genetic Variations
- Microbial metabolism and enzyme function
- Plant and Biological Electrophysiology Studies
- Plant and animal studies
- Mass Spectrometry Techniques and Applications
- Plant-Microbe Interactions and Immunity
- DNA Repair Mechanisms
- Synthesis and Biological Evaluation
- Lipid metabolism and biosynthesis
- Toxin Mechanisms and Immunotoxins
Czech Academy of Sciences, Institute of Experimental Botany
2007-2025
Palacký University Olomouc
2014-2025
Centre of the Region Haná for Biotechnical and Agricultural Research
2014-2017
University Hospital Olomouc
2001-2013
Max Planck Institute of Biochemistry
2007-2010
Max Planck Society
2007-2010
University of Basel
2009
We have developed a new analytical approach for isolation and quantification of cytokinins (CK) in minute amounts fresh plant material, which combines simple one-step purification with ultra-high performance liquid chromatography-fast scanning tandem mass spectrometry.Plant tissue samples (1-5 mg FW) were purified by stop-and-go-microextraction (StageTip purification), previously has only been applied clean-up pre-concentration peptides. found that combination two reverse phases one...
Argonaute (Ago) proteins are highly conserved between species and constitute a direct-binding platform for small RNAs including short-interfering (siRNAs), microRNAs (miRNAs) Piwi interacting (piRNAs). Small function as guides whereas Ago the actual mediators of gene silencing. Although major steps in RNA-guided silencing have been elucidated, not much is known about Ago-protein regulation. Here we report comprehensive analysis Ago2 phosphorylation human cells. We find that tyrosine Y529,...
Impaired DNA damage response pathways may create vulnerabilities of cancer cells that can be exploited therapeutically. One such selective vulnerability is the sensitivity BRCA1- or BRCA2-defective tumors (hence defective in repair by homologous recombination, HR) to inhibitors poly(ADP-ribose) polymerase-1 (PARP-1), an enzyme critical for alternative HR. While promising, treatment with PARP-1 (PARP-1i) faces some hurdles, including (1) acquired resistance, (2) search other sensitizing,...
A series of eight N ‐heterocyclic carbenes (NHC) gold(I) complexes, involving 1,3‐bis(2,6‐diisopropylphenyl)imidazol‐2‐ylidene (iPr) ligand in combination with 6‐mercaptopurine derivatives (HL 1–8 ), has been prepared and thoroughly characterized, including elemental analysis, mass spectrometry, infrared multinuclear NMR spectroscopy, single crystal X‐ray analysis. The showing general composition [Au (iPr)(L n )] 1 – 8 , were evaluated for their vitro cytotoxicity against four human cancer...
During mitosis, phosphorylation of spindle associated proteins is a key regulatory mechanism for formation, mitotic progression, and cytokinesis. In the recent past, mass spectrometry has been applied successfully to identify proteomes phosphoproteomes, but did not address their dynamics. Here, we present quantitative comparison phosphoproteomes prepared from different stages. total, report identification SILAC based relative quantitation 1940 unique sites find that late mitosis (anaphase,...
Summary Cyclic electron transport ( CET ) around photosystem I PSI plays an important role in balancing the ATP / NADPH ratio and photoprotection of plants. The NAD(P)H dehydrogenase complex NDH has a key function one pathways. Current knowledge indicates that, order to fulfill its , needs be associated with ; however, until now there been no direct structural information about such supercomplex. Here we present data obtained for plant – Electron microscopy analysis revealed that this...
Many proteins are present in the nucleus; some involved with its structural and functional organization, gene expression, cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both artifactual alteration of extent contamination non-nuclear proteins. conventional multi-step fractionation procedure is laborious prone to contamination. Here, we describe a single-step method based on flow sorting. allows separation...
We investigated the importance of protein nutrition for honey bee immunity. Different diets (monofloral pollen Helianthus spp., Sinapis Asparagus Castanea a mixture four different and substitute FeedbeeTM) were fed to bees in cages ad libitum. After 18 days feeding, apidaecin 1 isoforms concentration thorax measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels genes, coding apidaecins abaecin abdomen determined quantitative PCR. The results indicate...
Sister chromatids are held together by the ring-shaped cohesin complex, which likely entraps both DNA-double strands in its middle. This tie is resolved anaphase when separase, a giant protease, becomes active and cleaves kleisin subunit of cohesin. Premature activation separase and, hence, chromosome missegregation prevented at least two inhibitory mechanisms. Although securin has long been appreciated as direct inhibitor surprisingly loss basically no phenotype mammals....
Almost 25 years ago, an enzyme named zeatin cis trans isomerase from common bean has been described by Bassil et al. in Plant Physiol. (1993) 102: 867-872. The partially purified required external addition of FAD and dithiothreitol for the conversion cis-zeatin to its trans- isomer that occurred only under light. Although existence this important involved metabolism plant hormones cytokinins was generally accepted biologists, corresponding protein encoding gene have not identified date....
Abstract Bisimidazolium salts with one central biphenyl binding site and two terminal adamantyl sites form water‐soluble binary or ternary aggregates cucurbit[7]uril (CB7) β‐cyclodextrin (β‐CD) rotaxane pseudorotaxane architectures. The observed arrangements result from cooperation of the supramolecular stopper strength steric barriers against free slippage CB7 β‐CD host molecules over bisimidazolium guest axle.
Photosystem II (PSII) complexes are organized into large supercomplexes with variable amounts of light-harvesting proteins (Lhcb). A typical PSII supercomplex in plants is formed by four trimers Lhcb (LHCII trimers), which bound to the core dimer via monomeric antenna proteins. However, architecture Norway spruce[Picea abies (L.) Karst.] different, most likely due a lack two proteins, Lhcb6 and Lhcb3. Interestingly, spruce shares similar structural features its counterpart green alga...