A5030011233- Cancer-related Molecular Pathways
- Crystallization and Solubility Studies
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- X-ray Diffraction in Crystallography
- Fungal Plant Pathogen Control
- Plant tissue culture and regeneration
- Antimicrobial Resistance in Staphylococcus
- Mycotoxins in Agriculture and Food
- Synthesis and Biological Evaluation
- Plant Molecular Biology Research
- Bioactive Compounds and Antitumor Agents
- DNA Repair Mechanisms
- Calcium signaling and nucleotide metabolism
- Microbial Metabolites in Food Biotechnology
- Genetics and Neurodevelopmental Disorders
- Biochemical and Structural Characterization
- Advanced Breast Cancer Therapies
- Genomics and Phylogenetic Studies
- Microtubule and mitosis dynamics
- Toxin Mechanisms and Immunotoxins
- Agriculture, Plant Science, Crop Management
- Adenosine and Purinergic Signaling
- Wheat and Barley Genetics and Pathology
- Cancer Mechanisms and Therapy
Palacký University Olomouc
2012-2024
Institute of Molecular and Translational Medicine
2016-2024
Czech Academy of Sciences, Institute of Experimental Botany
2010-2021
University Hospital Olomouc
2016-2017
Masaryk University
2005
Cyclin-dependent kinases (CDKs) are an important and emerging class of drug targets for which many small-molecule inhibitors have been developed. However, there is often insufficient data available on the selectivity CDK (CDKi) to attribute effects presumed target these inhibitors. Here, we highlight discrepancies between kinase CDKi phenotype exhibited; evaluated 31 (claimed CDK1–4) activity toward CDKs 1, 2, 4, 5, 7, 9 cell cycle. Our results suggest that most should be reclassified as...
Inhibition of cyclin-dependent kinases (CDKs) with small molecules has been suggested as a strategy for treatment cancer, based on deregulation CDKs commonly found in many types human tumors. Here, new potent CDK2 inhibitor pyrazolo[4,3-d]pyrimidine scaffold synthesized, characterized, and evaluated cellular biochemical assays. 7-Benzylamino-5(R)-[2-(hydroxymethyl)propyl]amino-3-isopropyl-1(2)H-pyrazolo[4,3-d]pyrimidine, compound 7, was prepared bioisostere the well-known CDK roscovitine. An...
This study aimed to characterise compounds with activity against carbapenemase-expressing Gram-negative bacteria and nematodes evaluate their cytotoxicity non-cancerous human cells. The antimicrobial toxicity of a series phenyl-substituted urea derivatives were evaluated using broth microdilution, chitinase, resazurin reduction assays. effects different substitutions present on the nitrogen atoms backbone investigated. Several active Staphylococcus aureus Escherichia coli control strains....
Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve efficiency agriculture plant tissue culture-based practices. Here, we report a synthesis structure-activity relationship study novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, lowest value yet documented. Other isoforms Arabidopsis were also inhibited very effectively. binding mode...