A5022174246- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Influenza Virus Research Studies
- interferon and immune responses
- Parvovirus B19 Infection Studies
- Biosensors and Analytical Detection
- Respiratory viral infections research
- Crystal structures of chemical compounds
- Synthesis of β-Lactam Compounds
- Immune Response and Inflammation
- Pharmacological Effects of Natural Compounds
- Rabies epidemiology and control
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Plant tissue culture and regeneration
- Hemoglobin structure and function
- Metal complexes synthesis and properties
- Advanced biosensing and bioanalysis techniques
- HIV Research and Treatment
- Toxoplasma gondii Research Studies
- Advanced Chemical Sensor Technologies
Biomedical Research Center of the Slovak Academy of Sciences
2020-2024
Institute of Virology of the Slovak Academy of Sciences
2019-2024
Comenius University Bratislava
2015-2024
The oncogenic gammaherpesviruses, Epstein-Barr virus (EBV) and Kaposi's sarcoma herpesvirus (KSHV), are etiologically associated with a variety of human cancers, including Burkitt's lymphoma (BL), Hodgkin (HL), (KS), primary effusion (PEL). Recently, we demonstrated KSHV infection B- endothelial cells to significantly upregulate the expression interferon induced transmembrane protein 1 (IFITM1) which in turn enhances entry. This is an extension above study. In here, determined EBV trigger...
Murine gammaherpesvirus 68 (MHV-68) provides a valuable tool to screen novel therapeutic strategies against oncogenic gammaherpesviruses. The development and characterization of antiviral agents usually depend on appropriate screening assays. aim this study was develop rapid sensitive method for testing compounds For purpose, recombinant MHV-68 expressing firefly luciferase (MHV-68/LUC) constructed. conditions MHV-68/LUC infection in Vero cells suitable assay 96-well plate format were then...
Abstract In vivo bioluminescence imaging (BLI) offers a unique opportunity to analyze ongoing bacterial infections qualitatively and quantitatively in intact animals over time, leading reduction the number of needed for study. Since accurate determination burden plays an essential role microbiological research, present study aimed evaluate ability quantify bacteria by non-invasive BLI technique comparison standard spread plate method reverse transcription quantitative PCR (RT-qPCR). For this...
Ecology and epidemiology of murine gammaherpesvirus 68 (MHV-68) have been intensively studied since the isolation virus from murid rodents in 1976. This was detected various mammalian species that share biotope with rodent reservoirs MHV-68. However, a survey MHV-68 birds has not so far performed. Therefore, aim this study to investigate presence blood samples two bird captured at four localities Slovakia. Using nested PCR targeting ORF50 gene MHV-68, we confirmed DNA 9 out 57 Great tits...
We demonstrated the positive effect of Isoprinosine treatment on persistent infection Balb/c mice with murine gammaherpesvirus 68 (MHV-68). Increased number leukocytes, increased percentage neutrophils, elevated levels virus-neutralizing (VN) antibodies, reduced atypical lymphocytes and virus titers were detected in examined organs after a 14-day treatment. The therapy vanished 120-150 days. After this interval, we lower numbers VN antibodies an lymphoid monocytes Isoprinosine-treated group....
Background. Cyclin-dependent kinase 8 (CDK8) is a multifaceted regulator and represents catalytic component of the transcriptional Mediator complex. CDK8 activity, on one hand, increases elongation by recruitment Mediator/super complexes, but, other negatively regulates CDK7-controlled initiation through inactivating cyclin H phosphorylation. Recently, these combined properties have also suggested its rate-limiting importance for herpesviral replication. Objectives. In this paper, we focused...
Based on our previous results, which confirmed the role of latent gammaherpesvirus infection in alteration immune homeostasis, we studied influence simultaneous with gammaherpes and influenza viruses selected parameters innate immunity, particularly subpopulations peripheral blood cell leukocytes.The aim was to analyze changes differential count BALB/c mice persistently infected murine 68 (MHV-68) subsequently co-infected A virus (IAV), comparison MHV-68 or IAV only.Our results showed that...
Oseltamivir phosphate (OS) is currently the most frequently used influenza antiviral drug. It moderates course of virus type A (IAV) infection, however, its impact on induction virus-neutralizing antibodies (VNAbs) not understood in details. Here, we examined influence low (10 mg/kg) or high (60 doses OS viral titer lungs BALB/c mice infected with 0.5 LD50 IAV and level VNAbs. Prophylactic application (6 h before infection) delayed increase a lower peak comparison to non-treated control...