A5047503475- Cinema and Media Studies
- Plant Parasitism and Resistance
- Education, Psychology, and Social Research
- Plant Molecular Biology Research
- Plant and animal studies
- Cancer-related Molecular Pathways
- Race, History, and American Society
- European Cultural and National Identity
- Allelopathy and phytotoxic interactions
- Synthetic Organic Chemistry Methods
- Radical Photochemical Reactions
- Canadian Identity and History
- Oxidative Organic Chemistry Reactions
- Cancer therapeutics and mechanisms
- Eastern European Communism and Reforms
- Chronic Lymphocytic Leukemia Research
- Supramolecular Chemistry and Complexes
- Asymmetric Synthesis and Catalysis
- Insect-Plant Interactions and Control
- Photosynthetic Processes and Mechanisms
- Comics and Graphic Narratives
- Synthesis of heterocyclic compounds
- Recycling and utilization of industrial and municipal waste in materials production
- South Asian Studies and Conflicts
- Sulfur-Based Synthesis Techniques
Palacký University Olomouc
2015-2024
Czech Academy of Sciences, Institute of Experimental Botany
2014-2024
Centre of the Region Haná for Biotechnical and Agricultural Research
2018
Czech Academy of Sciences, Biology Centre
2014-2017
Brno University of Technology
2012-2014
Farmak (Czechia)
2011
Masaryk University
2002-2010
UCLouvain
2005-2009
University of Missouri
2003
The plant hormone auxin is believed to influence almost every aspect of growth and development. Auxin transport, biosynthesis degradation combine form gradients the that a range key developmental environmental response processes. There abundant genetic evidence for existence multiple pathways degradation. complexity these makes it difficult obtain clear picture relative importance specific metabolic during We have developed sensitive mass spectrometry-based method simultaneously profile...
Understanding the links between nucleophilic/reductive strength of environment, formation radicals and point defect characteristics is crucial for achieving control over functionalization fluorographene.
3,5,7-Trisubstituted pyrazolo[4,3-d]pyrimidines have been identified as potent inhibitors of cyclin-dependent kinases (CDKs), which are established drug targets. Herein, we describe their further structural modifications leading to novel nanomolar with strong antiproliferative activity. We determined the crystal structure fully active CDK2/A2 5-(2-amino-1-ethyl)thio-3-cyclobutyl-7-[4-(pyrazol-1-yl)benzyl]amino-1(2)H-pyrazolo[4,3-d]pyrimidine (24) at 1.7 Å resolution, confirming competitive...
The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic traceable analog to study auxin-related responses. Here we identified that not only exogenous but also its amide-linked metabolite 2,4-D-Glu displayed inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate conversion, identity activity, have developed novel purification procedure based combination of ion exchange immuno-specific sorbents combined with...
[reaction: see text] A novel modification of the classical Julia-Lythgoe olefination, using sulfoxides instead sulfones, affords, after in situ benzoylation and SmI2/HMPA- or DMPU-mediated reductive elimination, 1,2-di-, tri-, tetrasubstituted olefins moderate to excellent yields E/Z selectivity. The conditions are mild, procedure is broadly applicable.
Cyclin-dependent kinases are therapeutic targets frequently deregulated in various cancers. By convenient alkylation of the 5-sulfanyl group, we synthesized 3-isopropyl-7-[4-(2-pyridyl)benzyl]amino-1(2)H-pyrazolo[4,3-d]pyrimidines with substitutions at position 5 potent antiproliferative activity non-Hodgkin lymphoma cell lines. The most derivative 4.35 also displayed activities across more than 60 cancer kinase profiling confirmed high selectivity toward cyclin-dependent (CDKs) 2, 5, and 9,...
Real-world data on the long-term use of guselkumab for treatment psoriasis are still limited. We aimed to evaluate efficacy, safety, and drug survival in a real-world setting. This is retrospective study analyzing Czech Republic registry (BIOREP) patients treated with guselkumab. In total, 333 were included. Improvement Psoriasis Area Severity Index (PASI) score was significant. Mean PASI decreased from 16 at baseline 0.7, 0.9, 0.8 after 12, 24, 36 months, respectively. Absolute scores ≤ 3 1...
Abstract Jasmonates are a family of oxylipin phytohormones regulating plant development and growth mediating “defense versus growth” responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently CORONATIVE INSENSITIVE 1-mediated signaling in several stress-induced developmental processes. However, its perception metabolism only partially understood. An isoleucine analog the biologically active JA-Ile, OPDA-Ile, was detected years ago wounded...
Reaction of the antitumor agent leinamycin with cellular thiols results in conversion natural product to a DNA-alkylating episulfonium alkylating via an intriguing sequence chemical reactions. To establish whether chemistry first seen represents general motif that can function various molecular frameworks, construction greatly simplified analogues containing only "core" funcional groups anticipated be necessary for thiol-triggered generation was undertaken. For this purpose, "stripped-down"...
Irradiation of 2,5-dimethylbenzoyl oxiranes results in a relatively efficient and high-yielding formation β-hydroxy functionalized indanones that structurally resemble biologically active pterosines. Nanosecond laser flash photolysis quantum-chemical calculations based on density functional theory provided evidence this photochemical transformation proceeds primarily via photoenolization mechanism. Our study revealed considerable complexity the mechanism structural modifications can...
Targeting cyclin-dependent kinase 7 (CDK7) provides an interesting therapeutic option in cancer therapy because this participates regulating the cell cycle and transcription. Here, we describe a new trisubstituted pyrazolo[4,3-d]pyrimidine derivative, LGR6768, that inhibits CDK7 nanomolar range displays favourable selectivity across CDK family. We determined structure of fully active CDK2/cyclin A2 complex with LGR6768 at 2.6 Å resolution using X-ray crystallography, revealing conserved...
Abstract Bisimidazolium salts with one central biphenyl binding site and two terminal adamantyl sites form water‐soluble binary or ternary aggregates cucurbit[7]uril (CB7) β‐cyclodextrin (β‐CD) rotaxane pseudorotaxane architectures. The observed arrangements result from cooperation of the supramolecular stopper strength steric barriers against free slippage CB7 β‐CD host molecules over bisimidazolium guest axle.
Abstract Jasmonates (JAs) are a family of oxylipin phytohormones regulating plant development and growth mediating ‘defense versus growth’ responses. The upstream JA biosynthetic precursor cis -(+)-12-oxo-phytodienoic acid ( -OPDA) has been reported to act independently the COI1-mediated signaling in several stress-induced developmental processes. However, its means perception metabolism only partially understood. Furthermore, -OPDA, but not JA, occurs non-vascular species, such as...
Discadenine (1), a self-spore germination inhibitor from the cellular slim mold Dictyostelium discoideum, is structurally related to plant hormone cytokinin. This compound was synthesized l-aspartic acid, and its activities were confirmed by three classical cytokinin bioassays as well using binding activation assays with Arabidopsis receptors AHK3 CRE1/AHK4.