- Polysaccharides and Plant Cell Walls
- Food composition and properties
- Phytochemicals and Antioxidant Activities
- Microbial Metabolites in Food Biotechnology
- Fermentation and Sensory Analysis
- Biofuel production and bioconversion
- Polysaccharides Composition and Applications
- Lignin and Wood Chemistry
- Biochemical and biochemical processes
- Coffee research and impacts
- Polyamine Metabolism and Applications
- Essential Oils and Antimicrobial Activity
- Phytochemistry and Biological Activities
- Meat and Animal Product Quality
- Enzyme Production and Characterization
- Probiotics and Fermented Foods
- Tea Polyphenols and Effects
- Digestive system and related health
- biodegradable polymer synthesis and properties
- Spectroscopy and Chemometric Analyses
- Analytical Chemistry and Chromatography
- Mycotoxins in Agriculture and Food
- Metabolomics and Mass Spectrometry Studies
- Biopolymer Synthesis and Applications
- Identification and Quantification in Food
Karlsruhe Institute of Technology
2015-2024
Karlsruhe University of Education
2018-2024
Kentucky imaging Technologies (United States)
2024
University of Minnesota
2007-2013
Food and Nutrition Service
2011
German Institute of Human Nutrition
2007-2009
Universität Hamburg
2000-2009
University of Wisconsin–Madison
2000-2008
U.S. Dairy Forage Research Center
2000-2008
Agricultural Research Service
2000-2006
Abstract Cell wall cross‐linking can have a substantial effect on the properties of wall. To estimate (between arabinoxylans) in cereal fibres, dehydrodiferulate levels were measured soluble and insoluble dietary fibre (SDF IDF) isolated from whole grains maize ( Zea mays L), wheat Triticum aestivum spelt spelta rice Oryza sativa wild Zizania aquatica barley Hordeum vulgare rye Secale cereale oat Avena L) millet Panicum miliaceum L). After saponification fibres extracts investigated for...
Ferulate dehydrodimers and the more recently discovered dehydrotrimers play an important role in cell wall architecture of plant-based foods forages. High-performance liquid chromatography methods to determine ferulate dimers often lack specificity; for trimers did not exist yet. A method determination 11 wall-bound -trimers was developed, including crucial separation di/trimers from dominating phenolic monomers. Validation parameters basic calibration met our acceptance criteria. However,...
Pectins are dietary fibers that attributed with several beneficial immunomodulatory effects. Depending on the degree of esterification (DE), pectins can be classified as high methoxyl pectin (HMP) or low (LMP). The aim this study was to investigate effects methyl-esterification intestinal microbiota and its properties in naive mice. Supplementation diet LMP HMP induced changes composition mice toward Bacteroides, which mainly promoted by HMP. Metabolome analysis stool samples from pectin-fed...
Two new dehydrotriferulic acids and two dehydrotetraferulic were isolated from saponified maize bran insoluble fiber using size exclusion chromatography on Bio-Beads S-X3 followed by Sephadex LH-20 semipreparative phenyl-hexyl reversed phase high-performance liquid chromatography. On the basis of UV spectroscopy, mass spectrometry, one- two-dimensional NMR experiments, structures identified as 8-5(noncyclic)/5-5-dehydrotriferulic acid, 8-8(tetrahydrofuran)/5-5-dehydrotriferulic...
Phenolic compounds are incorporated into coffee melanoidins during roasting mainly in condensed form (42-62 mmol/100 g) and also ester-linked (1.1-1.6 g), with incorporation levels depending on the green chlorogenic acid content. The phenolic different melanoidin populations, but those soluble 75% ethanol (82%), a significant correlation between amount of protein color characteristics populations being observed. is pathway degradation roasting, representing 23% acids lost. These account for...
To analyze the accuracy of Klason lignin method as applied for determination contents in plant based-food products, preparations from curly kale, pears, whole wheat grains, and corn bran were chemically characterized. Characterization included routine ash protein determinations extraction fat/waxes well cutin/suberin depolymerization liberated monomers. Fat/wax amounts determined gravimetrically, their compositions analyzed by using GC-MS. Typical fat, wax, cutin (and suberin) constituents...
It is widely known that numerous adaptive responses of drought-stressed plants are stimulated by chemical messengers as phytohormones. Jasmonic acid (JA) one such kind phytohormone. But there very few reports revealing its direct implication in drought related or cross-talk with other In this study, we compared the morpho-physiological traits and root proteome a wild type (WT) rice plant JA biosynthesis mutant coleoptile photomorphogenesis 2 (cpm2), disrupted allene oxide cyclase (AOC) gene,...
Dietary fiber is a potential replacement for other ingredients such as starch in reformulated extruded breakfast cereals. Analysis of chokeberry pomace powder revealed total dietary content 57.8 ± 2 g/100 g with 76% being insoluble, 20% high molecular soluble and 4% low fiber. The polysaccharide composition was analyzed detail by using variety analytical approaches. Extrusion-like processing conditions were studies Closed Cavity Rheometer enabling the application defined thermal (temperature...
Salicylic acid is a phenolic phytohormone which controls plant growth and development. A methyl ester (MSA) derivative thereof volatile involved in plant-insect or plant-plant communication. Here we show that the nematode-trapping fungus Duddingtonia flagrans uses methyl-salicylic isomer, 6-MSA as morphogen for spatiotemporal control of trap formation chemoattractant to lure Caenorhabditis elegans into fungal colonies. product polyketide synthase an intermediate biosynthesis arthrosporols....
Coffee silver skin is produced in large amounts as a by-product during the coffee roasting process. In this study, of species Coffea arabica L. and canephora Pierre ex A. Froehner well pellets industry were characterized with respect to both nutritional value potential heat-induced contaminants. Enzymatic-gravimetric/chromatographic determination dietary fiber content showed values ranging from 59 67 g/100 g comparably high portion soluble fiber, whereas low molecular weight was not...
Plant cell walls containing suberin or lignin in the human diet are conjectured to protect against colon cancer. To confirm existence of authentic cereal grain dietary fibers, DFRC (derivatization followed by reductive cleavage) method was applied different fibers. By cleavage diagnostic arylglycerol-beta-aryl (beta-O-4) ether linkages and identification liberated monolignols, it ascertained that lignins truly present grains. From ratios monolignols coniferyl alcohol sinapyl alcohol, is...
Two 8−8-coupled sinapic acid dehydrodimers and at least three sinapate−ferulate heterodimers have been identified as saponification products from different insoluble soluble cereal grain dietary fibers. The two 8−8-disinapates were authenticated by comparison of their GC retention times mass spectra with authentic synthesized methyl or ethyl sinapate using single-electron metal oxidant systems. highest amounts (481 μg/g) found in wild rice fiber. Model reactions showed that it is unlikely...
Arabinogalactans and galactomannans from coffee beverages are part of the dietary fiber complex. Chemical structures fermentability soluble obtained a standard filter beverage (Coffea arabica, origin Colombia, medium roasted) by human intestinal bacteria were investigated. One cup (150 mL) contained approximately 0.5 g (enzymatic−gravimetric methodology), 62% which polysaccharides. The remainder was composed Maillard reaction products other nonidentified substances. Galactomannans type II...
Compositional information for lignins in food is rare and concentrated on cereal grains brans. As are suspected to have important health roles the dietary fiber complex, confusing current derived from nonspecific lignin determination methods needs be augmented by diagnostic structural studies. For this study, fractions were isolated kiwi, pear, rhubarb, and, comparison, wheat bran insoluble fiber. Clean pear kiwi isolates allowed substantive profiling, but it suggested that significance of...
To investigate the structure and fermentability of high M(r) components coffee brews by human gut bacteria Arabica samples three different degrees roast (light, medium, dark) were used for drip brew preparations fractionation ultrafiltration with cut-offs. Total carbohydrates fractions ranged from 28.6 g/100 g to 56.7 g. Galactomannans arabinogalactans main polysaccharides made up between one-fourth one-half respective fraction. After 24 h incubation a fecal suspension all extensively...