A5041270461- Leaf Properties and Growth Measurement
- Remote Sensing in Agriculture
- Plant Virus Research Studies
- Enzyme Production and Characterization
- Smart Agriculture and AI
- Viral Infectious Diseases and Gene Expression in Insects
- Geological Formations and Processes Exploration
- Plant Molecular Biology Research
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Machine Learning and Data Classification
- Plant nutrient uptake and metabolism
- Gene expression and cancer classification
- Biofuel production and bioconversion
- Plant Growth Enhancement Techniques
- Spectroscopy and Chemometric Analyses
- Bioinformatics and Genomic Networks
- Electrochemical sensors and biosensors
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Diet, Metabolism, and Disease
Palacký University Olomouc
2022-2024
Regional Centre of Advanced Technologies and Materials
2024
Mendel University in Brno
2016-2017
Central European Institute of Technology
2014-2017
Czech Academy of Sciences, Institute of Biophysics
2016-2017
Abstract Putrescine (Put) is a promising small molecule‐based biostimulant to enhance plant growth and resilience, though its mode of action remains unclear. This study investigated the Put priming effect on Arabidopsis mutant lines ( Atadc1, Atadc2, Atnata1 , Atnata2 ) under control conditions salinity understand role in regulating growth. The Atadc2 mutant, characterized by reduced endogenous levels, showed insensitivity without enhancement, which was linked significant imbalances nitrogen...
Inhibitors of cytokinin oxidase/dehydrogenase (CKX) reduce the degradation cytokinins in plants, and this effect can be exploited agriculture plant tissue culture. In study, we examine structure-activity relationship two series CKX inhibitors based on diphenylurea. The compounds Series I were derived from recently published 3TFM-2HM 3TFM-2HE, identified key substituents with increased selectivity for maize ZmCKX1 ZmCKX4a over AtCKX2 Arabidopsis. II contained that further exceled inhibitory...
Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve efficiency agriculture plant tissue culture-based practices. Here, we report a synthesis structure-activity relationship study novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, lowest value yet documented. Other isoforms Arabidopsis were also inhibited very effectively. binding mode...
In the context of global climate change and increasing need to study plant response drought, there is a demand for easily, rapidly, remotely measurable parameters that sensitively reflect leaf water status. Parameters with this potential include those derived from spectral reflectance (R) chlorophyll fluorescence. As each these methods probes completely different characteristics, their sensitivity loss may differ in species and/or under circumstances, making it difficult choose most...
Saturation mutagenesis is a cornerstone technique in protein engineering because of its utility (in conjunction with appropriate analytical techniques) for assessing effects varying residues at selected positions on proteins' structures and functions. Site-directed degenerate primers the simplest most rapid saturation technique. Thus, it highly whether or not variation certain sites permissible, but necessarily time- cost-effective detailed assessment variations' effects. presented study we...
Enzyme kinetic measurements are important for the characterization and engineering of biocatalysts, with applications in a wide range research fields. The measurement initial reaction velocity is usually slow laborious, which motivated us to explore possibilities automating this process. Our model enzyme maize β-glucosidase Zm-p60.1. Zm-p60.1 plays significant role plant growth development by regulating levels active hormone cytokinin. belongs group hydrolytic enzymes. Members hydrolyze...
Abstract Phenotypes, defining an organism’s behaviour and physical attributes, arise from the complex, dynamic interplay of genetics, development, environment, whose interactions make it enormously challenging to forecast future phenotypic traits a plant at given moment. This work reports AMULET, modular approach that uses imaging-based high-throughput phenotyping machine learning predict morphological physiological hours days before they are visible. AMULET streamlines process by...
The CRE1/AHK4 cytokinin receptor is an important component of plants' hormone signaling systems, and compounds that can alter its activity have potential utility for studying the receptor's functions and/or developing new plant growth regulators. A high-throughput method was developed screening with agonist or antagonist properties towards in a single experiment using Nanodrop II liquid handling system 384-well plates. Potential ligands are screened directly, reporter which triggers...