A5017559605- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Bacterial Identification and Susceptibility Testing
- Probiotics and Fermented Foods
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Fungal Biology and Applications
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Mycotoxins in Agriculture and Food
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Enterobacteriaceae and Cronobacter Research
- Streptococcal Infections and Treatments
- Polar Research and Ecology
- Toxin Mechanisms and Immunotoxins
- Milk Quality and Mastitis in Dairy Cows
- Plant Pathogenic Bacteria Studies
- Cancer Research and Treatments
- Antimicrobial Peptides and Activities
Masaryk University
2016-2025
Seqirus (United States)
2017
University of the Sunshine Coast
2017
RMIT University
2017
Palacký University Olomouc
2001-2005
Temperate bacteriophages play an important role in the pathogenicity of Staphylococcus aureus, for instance, by mediating horizontal gene transfer virulence factors. Here we established a classification scheme staphylococcal prophages major Siphoviridae family based on integrase polymorphism. Seventy-one published genome sequences phages were clustered into distinct groups which related to chromosomal integration site and encoded content. Analysis three marker modules (lysogeny, tail, lysis)...
Phage therapy is increasingly put forward as a “new” potential tool in the fight against antibiotic resistant infections. During “Centennial Celebration of Bacteriophage Research” conference Tbilisi, Georgia on 26–29 June 2017, an international group phage researchers committed to elaborate expert opinion three contentious related issues that are hampering clinical progress field therapy. This paper explores and discusses bacterial resistance, training presence prophages production strains...
Sequencing of at least 13 Staphylococcus aureus isolates has shown that genomic plasticity impacts significantly on the repertoire virulence factors. However, genome sequencing does not reveal which genes are expressed by individual isolates. Here, we have therefore performed a comprehensive survey composition and variability S. exoproteome. This involved multilocus sequence typing, gene, prophage profiling multiplex PCR, proteomic analyses secreted proteins using 2-DE. Dissection...
Abstract Alphaproteobacteria, which are the most abundant microorganisms of temperate oceans, produce phage-like particles called gene transfer agents (GTAs) that mediate lateral exchange. However, mechanism by GTAs deliver DNA into cells is unknown. Here we present structure GTA Rhodobacter capsulatus (RcGTA) and describe conformational changes required for its ejection. The RcGTA resembles a tailed phage, but it has an oblate head shortened in direction tail axis, limits packaging capacity...
Bacteriophages from the family Myoviridae use double-layered contractile tails to infect bacteria. Contraction of tail sheath enables tube penetrate through bacterial cell wall and serve as a channel for transport phage genome into cytoplasm. However, mechanisms controlling contraction release phages with "double-layered" baseplates were unknown. We used cryo-electron microscopy show that binding Twort-like phi812 Staphylococcus aureus requires 210° rotation heterohexameric receptor-binding...
The genus Macrococcus is a close relative of the Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered material identified sp. by polyphasic taxonomic approach comparative genomics. Relevant phenotypic, genotypic chemotaxonomic methods divided analyzed strains into two separate clusters within Macrococcus. Comparative genomics four...
This perspective paper follows up on earlier communications bacteriophage therapy that we wrote as a multidisciplinary and intercontinental expert-panel when first met at conference hosted by the Eliava Institute in Tbilisi, Georgia 2015. In context of society is confronted with an ever-increasing number antibiotic-resistant bacteria, build previously made recommendations specifically address how Nagoya Protocol might impact further development therapy. By reviewing recently conducted case...
Cryo-EM reveals the genome ejection mechanism of bacteriophage P68, a potential phage therapy agent against Staphylococcus aureus .
Staphylococcus aureus is a serious human and veterinary pathogen in which new strains with increasing virulence antimicrobial resistance occur due to acquiring genes by horizontal transfer. It generally accepted that temperate bacteriophages play major role gene In this study, we proved the presence of various bacterial S. COL strain directly within phage particles via qPCR quantified their packaging frequency. Non-parametric statistical analysis showed transducing φ11, φ80 φ80α serogroup B,...
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources comprising stone fragments and sandy soil in James Ross Island, Antarctica. Here, we describe properties of a novel species the genus Staphylococcus that has 16S rRNA gene sequence nearly identical to saprophyticus However, compared S. next closest relatives, new demonstrates considerable phylogenetic distance at whole-genome level, with an average nucleotide identity <85% inferred DNA-DNA...
Abstract Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages increase the effectivity of phage preparations emerging phage-resistant strains. Here we described genomic diversity spontaneous host-range mutants kayvirus 812. Five mutant were isolated as rare plaques on Staphylococcus aureus The host range 812-derived was 42% higher than wild type, determined a set 186 methicillin-resistant S. strains representing...
Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that notable for its ability to form biofilm and high rates of antibiotic resistance. It serves as reservoir multiple antimicrobial resistance genes spread among the staphylococcal population by horizontal gene transfer such transduction. While phage-mediated transduction well studied in aureus, S. transducing phages have not been described detail yet. Here, we report characteristics four phages, 27,...
The preparation of a solid dosage form containing bacteriophages, which meets pharmaceutical requirements and ensures long-term stability the phage effect, is significant for implementing therapy in practice. A commonly used method processing phages into freeze-drying (so-called) freeze-dried cake; however, to date there have been no studies examining pharmacopeial parameters tablets with bacteriophages. In this study, we describe properties cocktail purified pseudomonal bacteriophage DSM...
ABSTRACT Macrococci are usually found as commensals on the skin and mucosa of animals have been isolated from mammal-derived fermented foods; however, they can also act opportunistic pathogens. Here, we used whole-genome sequencing, comparative genomics, extensive biotyping, MALDI-TOF mass spectrometry, chemotaxonomy to characterize Macrococcus sp. strains livestock human-related specimens. Based results polyphasic taxonomy, propose species psychrotolerans nov. (type strain NRL/St 95/376 T =...
A polyphasic identification approach was applied to a group of 11 novobiocin-resistant staphylococci isolated from human clinical materials. Phenotypic characteristics obtained by both commercial and conventional tests assigned eight strains as Staphylococcus xylosus three ambiguous S. xylosus/Staphylococcus equorum. In contrast biotyping, ribotyping with EcoRI HindIII restriction endonucleases whole-cell protein fingerprinting six analysed equorum, five succinus. Confirmation the done...
Given the great biological importance and high diversity of temperate Staphylococcus aureus bacteriophages, a method is needed for description their genomic structure. Here we have updated multiplex PCR strategy complex characterization S. phages family Siphoviridae. Based on comparative analysis available phage sequences, multilocus typing major modules genome was designed. The were classified basis genes integrase (10 types), anti-repressor (five replication proteins polA, dnaC dnaD (four...
Abstract Staphylococcus sciuri is a bacterial pathogen associated with infections in animals and humans, represents reservoir for the mecA gene encoding methicillin-resistance staphylococci. No S. siphophages were known. Here identification characterization of two temperate phages from Siphoviridae family designated ϕ575 ϕ879 are presented. The have icosahedral heads flexible noncontractile tails that end tail spike. genomes 42,160 41,448 bp long encode 58 55 ORFs, respectively, arranged...
The transduction mediated by bacteriophages is considered to be one of the primary driving forces in horizontal gene transfer staphylococci, which crucial their adaptation and successful evolution. For a effective, it generally accepted that recipient strain should susceptible transducing phage. In this study, we demonstrate plasmid DNAs are effectively transduced into Staphylococcus aureus strains spite insensitivity lytic action phage, provided these phages adsorb bacterial cells....