A5069118685- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Polar Research and Ecology
- Microbial infections and disease research
- Microbial Community Ecology and Physiology
- Advanced X-ray Imaging Techniques
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Mycobacterium research and diagnosis
- Plant and Fungal Interactions Research
- Plant Parasitism and Resistance
- Private Equity and Venture Capital
- Bacterial Identification and Susceptibility Testing
- Integrated Circuits and Semiconductor Failure Analysis
- Vector-Borne Animal Diseases
- Bacterial biofilms and quorum sensing
- Animal Disease Management and Epidemiology
- Microbial Natural Products and Biosynthesis
Thermo Fisher Scientific (Czechia)
2023
Masaryk University
2017-2020
The genus Macrococcus is a close relative of the Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered material identified sp. by polyphasic taxonomic approach comparative genomics. Relevant phenotypic, genotypic chemotaxonomic methods divided analyzed strains into two separate clusters within Macrococcus. Comparative genomics four...
Two Gram-stain-positive, coagulase-negative staphylococcal strains were isolated from abiotic sources comprising stone fragments and sandy soil in James Ross Island, Antarctica. Here, we describe properties of a novel species the genus Staphylococcus that has 16S rRNA gene sequence nearly identical to saprophyticus However, compared S. next closest relatives, new demonstrates considerable phylogenetic distance at whole-genome level, with an average nucleotide identity <85% inferred DNA-DNA...
Abstract Staphylococcus sciuri is a bacterial pathogen associated with infections in animals and humans, represents reservoir for the mecA gene encoding methicillin-resistance staphylococci. No S. siphophages were known. Here identification characterization of two temperate phages from Siphoviridae family designated ϕ575 ϕ879 are presented. The have icosahedral heads flexible noncontractile tails that end tail spike. genomes 42,160 41,448 bp long encode 58 55 ORFs, respectively, arranged...
Four rod-shaped and Gram-stain-negative bacterial strains, CCM 8647, 8649T, 8643T 8648T, were isolated from rock samples collected on James Ross Island, Antarctica. Extensive biotyping, fatty acid profiling, chemotaxonomy, 16S rRNA gene sequencing whole-genome was applied to isolates clarify their taxonomic position. Phylogenetic analysis based indicated that all four belonged the genus Hymenobacter. Strains 8649T 8647 most closely related Hymenobacter arizonensis OR362-8T (94.4 % sequence...
Members of the genus
A set of three psychrotrophic bacterial strains was isolated from different soil samples collected at the deglaciated northern part James Ross Island (Antarctica) in 2014. All isolates were rod-shaped, Gram-stain-negative, non-motile, catalase-positive and oxidase-negative, produced moderately slimy red-pink pigmented colonies on Reasoner's 2A (R2A) agar. polyphasic taxonomic approach based 16S rRNA gene sequencing, whole-genome automated ribotyping, MALDI-TOF MS, chemotaxonomy methods...
Correlative cryo-FLM-FIB milling is a powerful sample preparation technique for in situ cryo-ET. However, correlative workflows that incorporate precise targeting remain challenging. Here, we demonstrate the development and use of an integrated Fluorescence Light Microscope (iFLM) module within cryo-FIB-SEM to enable coordinate-based two-point 3D workflow. The iFLM guided regions interest coupled with automated process instrument allows efficient 9-12 ∼200 nm thick lamellae 24 hours. Using...
Journal Article Precise 3D Localization by Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET Get access Jae Yang, Yang Department of Biochemistry, University Wisconsin, Madison, WI, United StatesMidwest Center Cryo-Electron Tomography, States Search other works this author on: Oxford Academic Google Scholar Veronika Vrbovská, Vrbovská Thermo Fisher Scientific, Brno, Czech Republic Tilman Franke, Franke Bryan Sibert, Sibert StatesCryo-Electron Research Center,...
Bacteriophages of the significant veterinary pathogen Staphylococcus pseudintermedius are rarely described morphologically and genomically in detail, mostly include phages Siphoviridae family. There is currently no taxonomical classification for this bacterial species. Here we describe a new phage designated vB_SpsS_QT1, which related to 2638A originally as aureus phage. Propagating strain S. 2854 latter was reclassified by rpoB gene sequencing work. Both have narrow but different host range...
Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) strains cause life-threatening diseases. We present a draft genome sequence of PVL-positive MRSA type 154 (ST154) strain NRL 08/001, isolated from fatal case necrotizing pneumonia. The consists 2.9 Mb over 39 contigs and harbors novel composite island staphylococcal cassette chromosome mec element (SCCmec)-mercury 2B&5.
Journal Article Fluorescence-guided Cryo-lift-out using an Integrated Fluorescence Light Microscope and Optimized Sample-needle Attachment Procedure Get access Veronika Vrbovská, Vrbovská Thermo Fisher Scientific, Brno, Czech Republic Search for other works by this author on: Oxford Academic Google Scholar Sven Klumpe, Klumpe Research Group CryoEM Technology, Max Planck Institute of Biochemistry, Martinsried, Germany Christopher Thompson, Thompson Eindhoven, Netherlands Alexander Rigort,...