A5050932325- Signaling Pathways in Disease
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Cell Function and Interaction
- Telomeres, Telomerase, and Senescence
- Sepsis Diagnosis and Treatment
- Immune responses and vaccinations
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Mindfulness and Compassion Interventions
- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Neuroblastoma Research and Treatments
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Tryptophan and brain disorders
- Olfactory and Sensory Function Studies
- Advanced biosensing and bioanalysis techniques
- Immunotherapy and Immune Responses
- Advanced Chemical Sensor Technologies
- Orthopedic Infections and Treatments
- COVID-19 Impact on Reproduction
- Inflammation biomarkers and pathways
- Antimicrobial Peptides and Activities
- Psychological Well-being and Life Satisfaction
St. Anne's University Hospital Brno
2017-2024
University Hospital Brno
2017-2024
International Clinical Research Center, St. Anne's University Hospital Brno
2024
Masaryk University
2017-2024
Background: Mindfulness-based programs have shown a promising effect on several health factors associated with increased risk of dementia and the conversion from mild cognitive impairment (MCI) to such as depression, stress, decline, immune system brain structural functional changes. Studies mindfulness in MCI subjects are sparse frequently lack control intervention groups. Objective: To determine feasibility mindfulness-based stress reduction (MBSR) practice cognition immunity compared...
Abstract Staphylococcus sciuri is a bacterial pathogen associated with infections in animals and humans, represents reservoir for the mecA gene encoding methicillin-resistance staphylococci. No S. siphophages were known. Here identification characterization of two temperate phages from Siphoviridae family designated ϕ575 ϕ879 are presented. The have icosahedral heads flexible noncontractile tails that end tail spike. genomes 42,160 41,448 bp long encode 58 55 ORFs, respectively, arranged...
Abstract Sepsis is characterized by dynamic changes of the immune system resulting in deregulated inflammation and failure homoeostasis can escalate to septic shock. Circulating monocytes other innate cells are among first ones recognize clear pathogens. Monocytes have an important role sepsis shock been studied as potential diagnostic markers. In total, forty‐two patients with were recruited blood samples obtained within 12 hours ICU admission. We showed that frequency classical...
Telomeropathies are rare disorders associated with impaired telomere length control mechanisms that frequently result from genetic mutations in the telomerase complex. Dyskeratosis congenita is a congenital progressive telomeropathy which mutation RNA component (TERC) impairs maintenance leading to accelerated cellular senescence and clinical outcomes resembling premature aging. The most severe feature perturbed hematopoiesis bone-marrow failure, but underlying not fully understood. Here, we...
COVID-19 manifestation is associated with a strong immune system activation leading to inflammation and subsequently affecting the cardiovascular system. The objective of study was reveal possible interconnection between prolongated development or exacerbation long-term complications after COVID-19. We investigated correlations humoral cellular markers together inflammation/dysfunction during onset subsequent recovery. analyzed 22 hospitalized patients severe within three timepoints (acute,...
Abstract Calcineurin–nuclear factor of activated T cells (CN-NFAT) inhibitors are widely clinically used drugs for immunosuppression, but besides their required cell response inhibition, they also undesirably affect innate immune cells. Disruption function can explain the observed susceptibility CN-NFAT inhibitor–treated patients to opportunistic fungal infections. Neutrophils play an essential role in immunity as a defense against pathogens; however, effect on neutrophil was poorly...
Calcineurin (CN) inhibitors are effective clinical immunosuppressants but leave patients vulnerable to potentially fatal fungal infections. This study tested the hypothesis that CN inhibition interferes with antifungal immune defenses mediated by monocytes. We showed NFAT is expressed human monocytes, and activated exposure ligands. confirmed translocation potently target gene transcription using a monocytic reporter cell line. Inhibition of CN-NFAT cyclosporine A significantly reduced...
Mesenchymal stromal cells (MSCs) combined with calcineurin-nuclear factor of activated T cell (CN-NFAT) inhibitors are being tested as a treatment for graft-versus-host disease (GvHD). The immunosuppressive properties MSCs seem beneficial; however, their response during fungal infection, which is an important cause mortality in patients GvHD, unknown. We report that phagocytose the component zymosan, resulting phosphorylation spleen tyrosine kinase (Syk), increase cytosolic calcium levels,...
Sepsis and septic shock remain leading causes of morbidity mortality for patients in the intensive care unit. During early phase, immune cells produce various cytokines to prompt activation system. Polymorphonuclear leukocytes (PMNs) respond different signals producing inflammatory factors executing their antimicrobial mechanisms, resulting engulfment elimination invading pathogens. However, excessive caused by produced during sepsis progression can lead alteration PMN signaling subsequent...
Background: Several years after the COVID–19 pandemic, role of trained immunity in remains controversial, and questions regarding long–term effects on immune cells remain unresolved. We investigated roles Bacillus Calmette–Guérin (BCG) vaccination latent infections progression sepsis. Methods: conducted a prospective analysis 97 individuals recovering from mild–to–critical 64 sepsis patients. Immune cell frequencies, expression functional markers, plasma titers anti–Toxoplasma...
Sepsis is a life-threatening condition characterised by an overwhelming immune response and high fatality. While most research has focused on its acute phase, many sepsis survivors remain immunologically weakened leaving them susceptible to serious complications from even mild infections. The mechanisms underlying this prolonged dysregulation unclear, limiting effective interventions. Here, we analysed whether induced long-term ″training″ in hematopoietic stem progenitor cells (HSPCs),...
Panton-Valentine leucocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) strains cause life-threatening diseases. We present a draft genome sequence of PVL-positive MRSA type 154 (ST154) strain NRL 08/001, isolated from fatal case necrotizing pneumonia. The consists 2.9 Mb over 39 contigs and harbors novel composite island staphylococcal cassette chromosome mec element (SCCmec)-mercury 2B&5.