A5035220446- Ultrasound and Hyperthermia Applications
- Ultrasound and Cavitation Phenomena
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Hydrogels: synthesis, properties, applications
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Supramolecular Self-Assembly in Materials
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- NMR spectroscopy and applications
- Advanced Polymer Synthesis and Characterization
- Nanoplatforms for cancer theranostics
- Material Dynamics and Properties
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pickering emulsions and particle stabilization
- Antimicrobial Peptides and Activities
- Electrochemical sensors and biosensors
- Proteins in Food Systems
- Electrochemical Analysis and Applications
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Microbial Inactivation Methods
- DNA and Nucleic Acid Chemistry
University of Rome Tor Vergata
2016-2025
RMIT University
2020-2025
MIT University
2020-2025
University of Padua
2018-2024
The University of Melbourne
2013-2023
Chiesi (France)
2015-2020
Australian Research Council
2017
Fondazione IRCCS Istituto Nazionale dei Tumori
2008
Rutherford Appleton Laboratory
2003-2008
Karolinska Institutet
2008
Oil-filled microcapsules of kraft lignin were synthesized by first creating an oil in water emulsion followed a high-intensity, ultrasound-assisted cross-linking at the water/oil interface. The rationale behind our approach is based on promoting documented hydrophobic interactions within phase, locking resulting spherical microsystems covalent using high intensity ultrasound treatment. As further evidence support rationale, confocal and optical microscopies demonstrated uniformly morphology...
The intracellular delivery of nucleic acids and proteins remains a key challenge in the development biological therapeutics. In gene therapy, inefficient small interfering RNA (siRNA) to cytosol by lipoplexes or polyplexes is often ascribed entrapment degradation siRNA payload endosomal compartments. A possible mechanism which rupture membrane release their acid cargo commonly defined as "proton sponge effect". This an osmosis-driven process triggered proton buffering capacity polyplexes....
The intracellular delivery of functional nanoparticles (NPs) and the release therapeutic payloads at a target site are central issues for biomedical applications. However, endosomal entrapment NPs typically results in degradation active cargo, leading to poor outcomes. Current advances promote escape largely involve use polycationic polymers cell-penetrating peptides (CPPs), which both can suffer from potential toxicity convoluted synthesis/conjugation processes. Herein, we report...
High-intensity ultrasound induces emulsification and cross-linking of protein molecules in aqueous medium. The stability the functionality resultant protein-coated microbubbles are crucial many their applications. For example, drug-loaded should be sufficiently long enough, vivo, so that they can ruptured only at specific sites for release drugs. In this study, we report synthesis stable functional microbubbles, coated with chemically reduced lysozyme, using high-intensity solution. absence...
Abstract Cardiovascular diseases remain the leading cause of death worldwide; hence there is an increasing focus on developing physiologically relevant in vitro cardiovascular tissue models suitable for studying personalized medicine and pre-clinical tests. Despite recent advances, that reproduce both complexity maturation are still limited. We have established a scaffold-free protocol to generate multicellular, beating human cardiac microtissues from hiPSCs—namely organotypic (hOCMTs)—that...
Abstract DNA‐based materials have attracted interest due to the tunable structure and encoded biological functionality of nucleic acids. A simple general approach synthesize with fine control over morphology bioactivity is important expand their applications. Here, we report synthesis particles via supramolecular assembly tannic acid (TA) DNA. Uniform different morphologies are obtained using a variety DNA building blocks. The enable co‐delivery cytosine‐guanine adjuvant sequences antigen...
Gas filled hollow microparticles, i.e., microbubbles and microballoons, are soft matter devices used in a number of diverse applications ranging from protein separation purification food science to drilling technology ultrasound imaging. Aqueous dispersions these mesoscopic systems characterized by the stabilization air/water interface thin shell phospholipid bilayer or multilayers denatured cross-linked proteic matrix. We present study type microballoons based on modified poly(vinyl...
We report the synthesis and characterization of DNA-grafted poly(N-isopropylacrylamide) (PNIPAM) micelles, their assembly into multilayered thin films, subsequent generation poly(ethylene glycol) (PEG) functionalization DNA−PNIPAM microcapsules. Multilayer films were assembled by sequentially depositing PNIPAM micelles containing cDNA sequences polyA30 or polyT30 (i.e., PNIPAM-A30 PNIPAM-T30). DNA−polymer microcapsules obtained alternate deposition PNIPAM-T30 onto silica particles, followed...
In this report, we describe the delivery of small interfering RNA (siRNA) using LbL-assembled microcapsules. The microcapsules are based on negatively charged poly(methacrylic acid) nanometer thin films containing cross-linking disulfide bonds. One system is polycation-free and another contains polylysine for siRNA complexation in microcapsule void. When a targeting survivin were delivered to PC-3 prostate cancer cells, significant inhibition expression antiapoptotic protein was observed....
Nanostructured materials have potential as platforms for analytical assays and catalytic reactions. Herein, we report the synthesis of electrocatalytically active cobalt phosphate nanostructures (CPNs) using a simple, low-cost, scalable preparation method. The electrocatalytic properties CPNs toward electrooxidation glucose (Glu) were studied by cyclic voltammetry chronoamperometry in relevant biological electrolytes, such phosphate-buffered saline (PBS), at physiological pH (7.4). Using...
We assembled dietary, bioactive flavonoids into a metal coordinated network to form thin, surface-bound films and hollow capsules, overcoming the poor water solubility of free flavonoids. Films formed from quercetin, myricetin, luteolin fisetin show radical scavenging activity, renowned feature their parent flavonoids, can be reused over multiple cycles. These are expected have potential applications in pharmaceutical food industries.
Abstract Interfacial self‐assembly is a powerful organizational force for fabricating functional nanomaterials, including nanocarriers, imaging and drug delivery. Herein, the interfacial of pH‐responsive metal–phenolic networks (MPNs) on liquid–liquid interface oil‐in‐water emulsions reported. Oleic acid 100–250 nm in diameter are generated by ultrasonication, to which poly(ethylene glycol) (PEG)‐based polyphenolic ligands assembled with simultaneous crosslinking metal ions, thus forming an...
The manipulation of interfacial properties has broad implications for the development high-performance coatings. Metal-phenolic networks (MPNs) are an emerging class responsive, adherent materials. Herein, host-guest chemistry is integrated with MPNs to modulate their surface and properties. Macrocyclic cyclodextrins (host) conjugated catechol or galloyl groups subsequently used as components assembly functional MPNs. assembled cyclodextrin-based highly permeable (even high molecular weight...
Glucose-responsive insulin-delivery platforms that are sensitive to dynamic glucose concentration fluctuations and provide both rapid prolonged insulin release have great potential control hyperglycemia avoid hypoglycemia diabetes. Here, biodegradable charge-switchable phytoglycogen nanoparticles capable of glucose-stimulated engineered. The "nanosugars" bearing glucose-sensitive phenylboronic acid groups amine moieties allow effective complexation with (≈95% loading capacity) form...
Abstract Nanoparticle‐mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and trafficking engineered nanoparticles. Unravelling molecular arrangements within nanoparticles as well their behavior are essential for designing effective nucleic acid systems. Herein, simple general strategy probing endosomal escape carrying in live cells reported. A triplex‐forming oligonucleotide probe designed to target transcription factor,...
Micro- and nanoparticles are considered suitable drug delivery systems for their unique features, such as a large surface to volume ratio, the possibility tune size hydrophobicity. A polymer/polymer/water emulsion method was used producing chemically cross-linked hydrogel made of poly(vinyl alcohol) poly(methacrylate) moieties. Mesoscopic investigation microparticles accomplished by laser scanning confocal microscopy. Dynamics confined water within gel meshes studied quasi-elastic incoherent...
A topologically extended model of a chemically cross-linked hydrogel poly(vinyl alcohol) (PVA) at high hydration degree has been developed for molecular dynamics simulation with atomic detail 323 K. The analysis the 5 ns trajectory discloses structural and dynamic aspects polymer solvation elucidates water hydrogen bonding diffusion in network. features local indicate that PVA mobility is not affected by constraints chemical junctions investigated cross-linking density, prevailing dumping...
Nitric oxide plays a central role in controlling arterial thrombosis and cardiovascular diseases by inhibiting the platelet aggregation process. This process is regulated giving deactivating signal for protein membrane integrins, major adhesion receptors. The localized production of NO, naturally occurring vessels, carried out NO synthase enzymatic system. Inhibition platelets coagulation cascade due to antagonist action toward integrin-fibrinogen induced adhesion. However, sometimes natural...
A sustainable, reagent-less and one-pot ultrasonic methodology has been developed to transform amorphous tannic acid into regularly shaped crystalline ellagic particles.
Abstract Self‐assembled structures obtained from organic molecules have shown great potential for applications in a wide range of domains. In this context, short peptides prove to be particularly versatile class building blocks self‐assembled materials. These species afford the biocompatibility and polymorphic richness typical proteins while allowing synthetic availability robustness smaller molecules. At nano‐to‐mesoscale, architectures peptide units exhibit stability large variety...
We report the microencapsulation of oil soluble vitamins (A, D and E) using a one pot ultrasonic process raw egg white proteins as shell material. Green tea catechin/iron complex coating method was further developed to impart UV filtering property microcapsules in order protect encapsulated nutrients from photodegradation. The showed antibacterial properties long shelf-life. were protected degradation upon heating, irradiation, simulated storage/transit cooking processes. in-vitro digestion...