The intracellular delivery of nucleic acids and proteins remains a key challenge in the development biological therapeutics. In gene therapy, inefficient small interfering RNA (siRNA) to cytosol by lipoplexes or polyplexes is often ascribed entrapment degradation siRNA payload endosomal compartments. A possible mechanism which rupture membrane release their acid cargo commonly defined as "proton sponge effect". This an osmosis-driven process triggered proton buffering capacity polyplexes....

ConspectusBiocompatible hydrogels are materials that hold great promise in medicine and biology since the porous structure, ability to entrap a large amount of water, tunability their mechanical tissue adhesive properties make them suitable for several applications, including wound healing, drug cell delivery, cancer treatment, bioelectronics, regeneration. Among possible developed systems, injectable hydrogels, owing properties, optimal candidates vivo minimally invasive procedures. To be...

Abstract The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics genetic engineering. However, the process mostly involves tedious techniques often requires extensive alteration protein itself. Herein we report straightforward approach encapsulate native by using breakable organosilica matrices that disintegrate upon exposure chemical stimulus. biomolecule‐containing capsules were tested intracellular...

Mesoporous silica nanoparticles (MSNPs), 100 nm in size, incorporating a Cy5 fluorophore within the framework, are synthesized and loaded with anti-cancer drug temozolomide (TMZ), used treatment of gliomas. The surface particles is then decorated, using electrostatic interactions, polyarginine-peptide nucleic acid (R8-PNA) conjugate targeting miR221 microRNA. multi-functional nanosystem thus obtained rapidly internalized into glioma C6 or T98G cells. anti-miR activity PNA retained, as...

There is an urgent need for new materials to treat bacterial infections. In order improve antibacterial delivery, anti‐infective nanomaterial developed that utilizes two strategies localization: i) a biodegradable nanoparticle carrier localize therapeutics within the tissue, and ii) novel tandem peptide cargo payload membranes. First, library of peptides screened combines membrane‐localizing with toxic discovers displays synergy between domains able kill Pseudomonas aeruginosa at...

Efficient and timely testing has taken center stage in the management, control, monitoring of current COVID-19 pandemic. Simple, rapid, cost-effective diagnostics are needed that can complement polymerase chain reaction-based methods lateral flow immunoassays. Here, we report development an electrochemical sensing platform based on single-walled carbon nanotube screen-printed electrodes (SWCNT-SPEs) functionalized with a redox-tagged DNA aptamer specifically binds to receptor binding domain...

Silencing of aberrantly expressed microRNAs (miRNAs or miRs) has emerged as one the strategies for molecular targeted cancer therapeutics. In particular, miR-21 is an oncogenic miRNA overexpressed in many tumors, including ovarian cancer. To achieve efficient administration anti-miR therapeutics, delivery systems are needed that can ensure local accumulation tumor environment, low systemic toxicity, and reduced adverse side effects. order to develop improved therapeutic agent treatment...

Abstract Point‐of‐care technologies need particularly stringent requirements, including portability, rapidity, affordability, and ease of use, enabling healthcare practitioners to perform reliable analyses in decentralized settings. Such diagnostic tests are usually based on bioassays that allow for the detection clinically relevant biomarkers, namely proteins, antibodies nucleic acids. In context electrochemical biosensors, implementation magnetic beads offers unique advantages stemming...

We describe a novel sensing approach based on functionalized microstructured optical fiber-Bragg grating for specific DNA target sequences detection. The inner surface of fiber, where Bragg was previously inscribed, has been by covalent linking peptide nucleic acid probe targeting sequence bearing single point mutation implicated in cystic fibrosis (CF) disease. A solution an oligonucleotide (ON) corresponding to tract the CF gene containing mutated infiltrated inside fiber capillaries and...

The design and synthesis of smart nanomaterials can provide interesting potential applications for biomedical purposes from bioimaging to drug delivery. Manufacturing multifunctional systems in a way carry bioactive molecules, like peptide nucleic acids able recognize specific targets living cells, represents an achievement towards the development highly selective tools both diagnosis therapeutics. This work describes very first example use zeolite nanocrystals as nanocarriers deliver...

Abstract The design and synthesis of multifunctional nanomaterials could lead to applications relevant for biomedicine. Manufacturing porous particles make them able carry bioactive molecules into living cells represents a substantial goal towards the development powerful tools nanomedicine. This work describes first example using zeolite‐L crystals as nanocontainers simultaneously deliver DNA oligonucleotides organic cells. Multifunctional was prepared by filling pore system with guest...

Scaffolds made from biocompatible polymers provide physical cues to direct the extension of neurites and encourage repair damaged nerves. The inclusion neurotrophic payloads in these scaffolds can substantially enhance regrowth processes. However, many promising candidates are excluded this approach due incompatibilities with polymer or processing conditions. This work provides one solution problem by incorporating porous silicon nanoparticles (pSiNPs) that pre-loaded therapeutic into a...

CRISPR-CAS systems have advanced many domains in life sciences, enabling diverse applications gene editing, diagnostics, and biosensing. Here, we introduce a platform that leverages transcription factors (TFs) to regulate CRISPR-Cas12a trans-cleavage activity via engineered DNA translators. These dynamic structures respond TF binding by switching conformations, modulating Cas12a activity. Using TATA-binding protein (TBP) Myc-Max as models, optimized translators for precise tunable control...

Abstract The growing ability of high‐performance consumer electronics is fostering innovation in prototyping and research. Although the Raspberry Pi single‐board computer (SBC) widely used across various fields, including diagnostics, applications involving UV‐based reading remain underexplored. In this study, a simple, low‐cost (less than 100 euros), portable device for UV–vis measurements based on presented. system integrates AS7341 multi‐channel multispectral digital sensor, offering...

Therapeutic drug monitoring (TDM) is increasingly valuable for tailoring personalized therapy, particularly in managing chronic inflammatory diseases where overtreatment can cause significant side effects. Monoclonal antibodies (mAbs), a primary therapeutic approach these conditions, face challenges from antidrug (ADAs), which reduce mAb bioavailability and efficacy. To address issues, we utilized Tumor Necrosis Factor α (TNF-α) as binding moiety fluorescence-based programmable nanosensor...

Integrating dynamic DNA nanotechnology with protein-controlled actuation will expand our ability to process molecular information. We have developed a strategy actuate strand displacement reactions using DNA-binding proteins by engineering synthetic translators that convert specific protein-binding events into trigger inputs through programmed conformational change. constructed networks responsive two different proteins, TATA-binding protein and Myc-Max, demonstrated multi-input activation...

Liquid biopsy is a non-invasive and efficient technique for the detection of tumor biomarkers in biological fluids, which currently represents new frontier theranostics precision medicine. Among mutations KRAS oncogene, p.G12D single nucleotide variation gene plays central role early diagnosis therapeutic treatment colorectal cancer. In this context, we developed highly sensitive magneto-genosensing assay based on PNA capture probes immobilized magnetic microbeads. To detect mutation two...

The development of smart nanoparticles (NPs) that encode responsive features in the structural framework promises to extend applications NP-based drugs, vaccines, and diagnostic tools. New nanocarriers would ideally consist a minimal number biocompatible components exhibit multiresponsive behavior specific biomolecules, but progress is limited by difficulty synthesizing suitable building blocks. Through nature-inspired approach combines programmability nucleic acid interactions sol–gel...

Organosilica nanoparticles that contain responsive organic building blocks as constitutive components of the silica network offer promising opportunities for development innovative drug formulations, biomolecule delivery, and diagnostic tools. However, synthetic challenges required to introduce dynamic multifunctional have hindered realization biomimicking nanoparticles. In this study, capitalizing on our previous research nucleic acid-based organosilica nanoparticles, we combine...

One of the most intriguing ways through which nature achieves regulation biological pathways encompasses coordination noncovalent interactions that bring biomolecules to be colocalized in a designated restricted space. Inspired by this mechanism, we have explored possibility using antibodies as bivalent biomolecular substrates for templated assembly functional RNA structure. We developed biosupramolecular complementation assay assembling fluorescent Spinach aptamer, is synthetic mimic Green...

An outlook on the current status of different strategies for magnetic micro- and nanosized bead functionalization with aptamers as prominent bioreceptors is given a focus electrochemical optical apta-assays, well aptamer-modified bead-based miniaturized extraction techniques in food control. Critical aspects that affect interaction target molecules, possible side effects caused by aptamer other molecules due to non-specific binding, are discussed. Challenges concerning real potential...

Transcription factor DNA binding activity is of pivotal importance in living systems because its primary involvement the regulation genetic machinery. The analysis transient expression levels transcription factors response to a certain cell status powerful means for investigating cellular dynamics at biomolecular level. Herein, DNA-based molecular switch that enables probing directly used cells. We demonstrate nanoswitch allows dynamic fluorescence imaging NF-κB and quantification downstream...

Abstract The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics genetic engineering. However, the process mostly involves tedious techniques often requires extensive alteration protein itself. Herein we report straightforward approach encapsulate native by using breakable organosilica matrices that disintegrate upon exposure chemical stimulus. biomolecule‐containing capsules were tested intracellular...