A5032987023- Corneal surgery and disorders
- Intraocular Surgery and Lenses
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Connexins and lens biology
- Ophthalmology and Visual Impairment Studies
- Photoacoustic and Ultrasonic Imaging
- Ocular and Laser Science Research
- Optical measurement and interference techniques
- Force Microscopy Techniques and Applications
- Advanced Measurement and Metrology Techniques
- Optical Imaging and Spectroscopy Techniques
- Near-Field Optical Microscopy
- Cellular Mechanics and Interactions
- Tactile and Sensory Interactions
- Urinary Bladder and Prostate Research
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Veterinary Equine Medical Research
- Neuroscience and Neural Engineering
- Thermography and Photoacoustic Techniques
- Electrospun Nanofibers in Biomedical Applications
- Ergonomics and Musculoskeletal Disorders
- Diabetes and associated disorders
University of Miami
2016-2025
American Orchid Society
2019
University of Crete
2013
purpose. To quantify the forces necessary to change shape and optical power of human monkey lenses. methods. Cynomolgus (n = 48; age: 3.8–11 years), rhesus 35; 0.7–17 years) 20, age 8–70 eyes obtained postmortem, including lens, capsule, zonules, ciliary body, sclera were mounted in an optomechanical lens-stretching system. Starting at zero load, lenses symmetrically stretched a stepwise fashion 0.25- or 0.5-mm steps. The lens diameter, inner body measured each step diameter- power-load...
We report a water-soluble and non-toxic method to incorporate additional extracellular matrix proteins into gelatin hydrogels, while obviating the use of chemical crosslinkers such as glutaraldehyde.
To characterize the age dependence of isolated human crystalline lens power and quantify contributions surfaces refractive index gradient.Experiments were performed on 100 eyes 73 donors (average 2.8 +/- 1.6 days postmortem) with an range 6 to 94 years. Lens was measured a modified commercial lensmeter or optical system based Scheiner principle. The radius curvature asphericity by shadow photography. For each lens, gradient calculated using ray-tracing software. dependency these powers...
Vascular disease is one of the leading causes death worldwide. repair, essential for tissue maintenance, critically reduced during vascular and aging. Efficient repair requires functional adult stem cells unimpaired by aging or mutation. One protein candidate reducing cellâmediated progerin, an alternative splice variant lamin A. Progerin results from erroneous activation cryptic sites within LMNA gene, significantly increases Mutations triggering progerin overexpression cause premature...
PURPOSE: To assess the cut quality of anterior and posterior surfaces intrastromal refractive lenticules removed during small incision lenticule extraction (SMILE). METHODS: The VisuMax femtosecond laser (500 kHz; Carl Zeiss Meditec, Dublin, CA) was used to perform SMILE on 8 eyes 5 individuals correct only myopia (no cylinder). energy index 26 (equivalent an 130 nJ) with a 2.5 × μ m spot/track separation. diameter 6.5 mm minimum edge thickness 15 cap 7.3 intended 120 m. After treatment,...
Scaffolds made from biocompatible polymers provide physical cues to direct the extension of neurites and encourage repair damaged nerves. The inclusion neurotrophic payloads in these scaffolds can substantially enhance regrowth processes. However, many promising candidates are excluded this approach due incompatibilities with polymer or processing conditions. This work provides one solution problem by incorporating porous silicon nanoparticles (pSiNPs) that pre-loaded therapeutic into a...
Polyethylene glycol (PEG)–based conformal coating (CC) encapsulation of transplanted islets is a promising β cell replacement therapy for the treatment type 1 diabetes without chronic immunosuppression because it minimizes capsule thickness, graft volume, and insulin secretion delay. However, we show here that our original CC method, direct requiring exposure to low pH levels inclusion viscosity enhancers during coating, severely affected viability, scalability, biocompatibility in nonhuman...
Fungal keratitis has a poor prognosis given deep penetration into the corneal stroma. While Rose Bengal photodynamic antimicrobial therapy (RB-PDAT) is promising adjunct treatment for refractory cases, limits its effectiveness. This study explores depth of alternative photosensitizers to address this issue. Thirty-five human corneas were soaked 30 minutes in 0.0075% solution disodium (n = 5), lactone Erythrosin B Eosin Y Methylene blue or NaCl (control, n 5). Confocal microscopy was used...
To investigate the depth-dependent mechanical anisotropy of human corneal stroma at tissue (stroma) and molecular (collagen) level by using atomic force microscopy (AFM).Eleven donor corneas were dissected different stromal depths a microkeratome. Mechanical measurements performed in 15% dextran on surface exposed each sample custom-built AFM spectroscopy mode both microspherical (38-μm diameter) nanoconical (10-nm radius curvature) indenters 2-μm/s 15-μm/s indentation rates. Young's modulus...
Objectives: To determine the effect of hydration media on ex vivo corneal elasticity. Methods: Experiments were conducted 40 porcine eyes retrieved from an abattoir (10 each for phosphate-buffered saline (PBS), balanced salt solution, Optisol, 15% dextran). The epithelium was removed, and cornea excised with intact scleral rim placed in 20% dextran overnight to restore its physiological thickness. For media, corneas evenly divided into two groups: one other without. Corneas mounted onto a...
The aim of this study was to determine the roles collagen XII in regulation stromal hierarchical organization, keratocyte and corneal mechanics.The temporal spatial expression at postnatal days 4, 10, 30, 90, 150 were evaluated wild-type (WT) mice. role organization analyzed by measuring fibril diameter density, as well lamellar structure, within ultrastructural micrographs obtained from WT XII-deficient mice (Col12a1-/-). Keratocyte morphology networks assessed using actin staining with...
purpose. To determine whether changes in elastic properties of the lens capsule ex vivo with age contribute to forces necessary for accommodation. methods. Postmortem human (n = 22; average: 41 ± 17years; range: 6–7) and cynomolgus monkey 19; 7.7 1.8 years, 4.2–10) tissues including lens, capsule, zonules, ciliary body, sclera were mounted an optomechanical lens-stretching system. Starting at 0 load, was symmetrically stretched 2 mm 0.25-mm steps a speed 0.1 · s−1. The load diameter measured...
Abstract Islet transplantation within mechanically stable microcapsules offers the promise of long‐term diabetes reversal without chronic immunosuppression. Reinforcing ionically gelled network alginate (ALG) hydrogels with covalently linked polyethylene glycol (PEG) may create hybrid structures desirable mechanical properties. This report describes fabrication PEG‐ALG interpenetrating polymer networks and investigation microcapsule swelling, surface modulus, rheology, compression,...
To measure interspecies thickness differences in the central anterior and posterior capsules of postmortem crystalline lenses, by a technique that maintains anatomic integrity lens.Central capsule was measured with custom-built, noncontact optical system, using focus detection technique. Anterior lens measurements were performed on 22 human, 29 monkey, 34 New Zealand White rabbit intact lenses situ. Eyes prepared for bonding PMMA ring to sclera region ciliary body after conjunctiva, adipose,...
PURPOSE: To measure the elasticity of nucleus and cytoplasm human mesenchymal stem cells (MSCs) as well changes brought about by exposure to nicotine in vitro. METHODS: MSCs were synchronized G(0) stage cell cycle through serum deprivation techniques. The then treated with medium containing (0.1 µM, 0.5 1 µM). Atomic force microscopy was used Young's modulus both these cells. RESULTS: For unsynchronized cells, softer than cytoplasm, although this difference not found be statistically...
Abstract Elevated intraocular pressure (IOP) is a risk factor in glaucoma, group of irreversible blinding diseases. Endogenous lipids may be involved regulation IOP homeostasis. We present comparative fold analysis phospholipids and sphingolipids aqueous humour trabecular meshwork from human control vs primary open‐angle glaucoma mouse (normotensive) ocular hypertensive state. The disease state was based on ratiometric mass spectrometric data for above classes lipids. standardized vitro...