A5075431166- Respiratory Support and Mechanisms
- Sepsis Diagnosis and Treatment
- Hemodynamic Monitoring and Therapy
- Cardiac, Anesthesia and Surgical Outcomes
- Education, Psychology, and Social Research
- Cardiovascular Health and Disease Prevention
- Abdominal Surgery and Complications
- Cardiac Arrest and Resuscitation
- Aortic aneurysm repair treatments
- Intensive Care Unit Cognitive Disorders
- Mechanical Circulatory Support Devices
- Non-Invasive Vital Sign Monitoring
- Renal function and acid-base balance
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Airway Management and Intubation Techniques
- Diabetes Management and Research
- Heart Rate Variability and Autonomic Control
- Dialysis and Renal Disease Management
- Venous Thromboembolism Diagnosis and Management
- Nosocomial Infections in ICU
- Pneumonia and Respiratory Infections
- Clinical Nutrition and Gastroenterology
- Central Venous Catheters and Hemodialysis
- Inflammation biomarkers and pathways
- Blood Pressure and Hypertension Studies
St. Anne's University Hospital Brno
2012-2024
University Hospital Brno
2011-2024
Masaryk University
2013-2024
Na Homolce Hospital
2017
Resuscitation Council
2017
University of Veterinary Sciences Brno
2009
Charles University
1996-2008
University Hospital Plzen
1997-2008
St Ann’s Hospital
2001
Kuopio University Hospital
2000
Sepsis continues to be a major cause of death, disability, and health-care expenditure worldwide. Despite evidence suggesting that host genetics can influence sepsis outcomes, no specific loci have yet been convincingly replicated. The aim this study was identify genetic variants survival.
Abstract Background Ventilator-associated pneumonia caused by Pseudomonas aeruginosa (PA) in hospitalised patients is associated with high mortality. The effectiveness of the bivalent, bispecific mAb MEDI3902 (gremubamab) preventing PA nosocomial was assessed PA-colonised mechanically ventilated subjects. Methods EVADE (NCT02696902) a phase 2, randomised, parallel-group, double-blind, placebo-controlled study Europe, Turkey, Israel, and USA. Subjects ≥ 18 years old, ventilated, tracheally...
Abstract Background Pseudomonas aeruginosa infections are a serious threat in intensive care units (ICUs). The aim of this confirmatory, randomized, multicenter, placebo-controlled, double-blind, phase 2/3 study was to assess the efficacy, immunogenicity, and safety IC43 recombinant vaccine non-surgical ICU patients. Methods Eight hundred patients aged 18 80 years admitted with expected need for mechanical ventilation ≥ 48 h were randomized 1:1 either 100 μg or saline placebo, given two...
Introduction Major clinically relevant inflammatory events such as septic shock and severe COVID-19 trigger dynamic changes in the host immune system, presenting promising candidates for new biomarkers to improve precision diagnostics patient stratification. Hepcidin, a master regulator of iron metabolism, has been intensively studied many pathologies associated with system activation, however these data have never compared other clinical settings. Thus, we aimed reveal dynamics regulation...
Objective To test the hypothesis that dopexamine reduces postoperative mortality and morbidity in high-risk, major abdominal surgery patients, when given to fluid-resuscitated patients starting before operation continued for 24 hrs after surgery. Design Prospective, randomized, controlled, double-blind multicenter trial. Setting Intensive care units 13 hospitals from six European countries. Patients A total of 412 with predefined high-risk criteria, undergoing an expected duration at least...
Abstract Around half of people with severe COVID‐19 requiring intensive care unit (ICU) treatment will survive, but it is unclear how the immune response to SARS‐CoV‐2 differs between ICU patients that recover and those do not. We conducted whole‐blood immunophenotyping upon admission during their uncovered marked differences in circulating cell subsets. At admission, who later succumbed had significantly lower frequencies all memory CD8+ T subsets, resulting increased CD4‐to‐CD8...
Euglykemická ketoacidóza je vzácná, ale potenciálně fatální komplikace léčby SGLT2 inhibitory (glifloziny). Obdobná euglykemická se však vzácněji může vyskytnout i při užívání jiných antidiabetik či u stavů jako těhotenství nebo abúzus alkoholu. V textu popisujeme 3 případy vlastních pacientů s euglykemickou ketoacidózou asociovanou glifloziny a 1 případ asociovaný DPP4 inhibitorem sitagliptinem podrobně diskutujeme přítomné acidobazické poruchy odlišnost euglykemické ketoacidózy od klasické...
Abstract Sepsis is characterized by dynamic changes of the immune system resulting in deregulated inflammation and failure homoeostasis can escalate to septic shock. Circulating monocytes other innate cells are among first ones recognize clear pathogens. Monocytes have an important role sepsis shock been studied as potential diagnostic markers. In total, forty‐two patients with were recruited blood samples obtained within 12 hours ICU admission. We showed that frequency classical...
Abstract Background Euglycemic diabetic ketoacidosis associated with SGLT2 inhibitors is a rare, relatively new and potentially fatal clinical entity, characterized by metabolic acidosis normal or only moderately elevated glycemia. The mechanisms are not fully understood but involve increased ketogenesis complex renal dysfunction, resulting in both hyperchloremic acidosis. We report rare case of empagliflozin-associated profound hyperchloremia review its pathogenesis. Case presentation A...
It is recognized that administration of insulin with glucose decreases catabolic response in sepsis. The aim the present study was to compare effects two levels insulinaemia on metabolism and energy expenditure septic patients volunteers. Glucose uptake, oxidation storage, were measured, using indirect calorimetry, 20 stable 10 volunteers a two-step hyperinsulinaemic (serum 250 1250 mIU/l), euglycaemic (blood concentration 5 mmol/l) clamp. Differences between steps clamp (from serum mIU/l)...
BackgroundCardiopulmonary exercise testing parameters including ventilatory efficiency (VE/VCO2 slope) are used for risk assessment of lung resection candidates. However, many patients unable or unwilling to undergo exercise. VE/VCO2 slope is closely related the partial pressure end-tidal carbon dioxide (PETCO2). We hypothesized PETCO2 at rest predicts postoperative pulmonary complications.MethodsConsecutive candidates were included in this prospective multicenter study. Postoperative...
Introduction Ventilatory efficiency (V E /VCO 2 slope) has been shown superior to peak oxygen consumption (VO ) for prediction of post-operative pulmonary complications in patients undergoing thoracotomy. V slope is determined by ventilatory drive and ventilation/perfusion mismatch whereas VO related cardiac output arteriovenous difference. We hypothesized pre-operative predicts cardiovascular lung resection. Methods Lung resection candidates from a published study were evaluated post-hoc...
Sepsis is a clinical syndrome characterized by dysregulated response to infection. It represents leading cause of mortality in ICU patients worldwide. Although sepsis the point interest research for several decades, its management and patient survival are improving slowly. Monitoring biomarkers their combinations could help early diagnosis, estimation prognosis patient's stratification treatment. Circulating soluble endoglin (sEng) cleaved extracellular part transmembrane glycoprotein...
Euglykemická ketoacidóza asociovaná se SGLT2 inhibitory, též označovanými jako glifloziny, je vzácný, ale potenciálně fatální syndrom, charakterizovaný metabolickou acidózou s normální nebo jen mírně zvýšenou glykemií, převážně u diabetiků 2. typu. Kromě ketoacidózy na metabolické acidóze může významně podílet hyperchloremická acidóza. Relativní hypoglykemie navozená glifloziny a souběžný stresový stav vedou ke snížení hladiny inzulinu zvýšení glukagonu, kortizolu katecholaminů, což...
Myeloid-derived suppressor cells (MDSCs) are important regulators of immune processes during sepsis in mice. However, confirming these observations humans has been challenging due to the lack defined preparation protocols and phenotyping schemes for MDSC subsets. Thus, it remains unclear how MDSCs involved acute whether they have a role long-term complications seen survivors. Here, we combined comprehensive flow cytometry with unsupervised clustering using self-organizing maps identify three...
Heparin-induced thrombocytopenia is a life-threatening immune-mediated complication of unfractionated heparin therapy. Fondaparinux therapeutic alternative, but it has limited evidence for its use in patients on extracorporeal membrane oxygenation (ECMO). We present series three adult with COVID-19 ECMO who were diagnosed heparin-induced after 7–12 days treatment and switched to fondaparinux. was initiated an intravenous loading dose 5 mg, followed by 2.5 mg subcutaneously every 8–12 h....
Abstract: Serum amino grams and daily losses of glutamine (Gin) other acids (AAs) into diafiltrate were measured during the first 5 days continuous venovenous hemodiafiltration (CVVHDF) in 6 ICU patients with acute renal failure (ARF). Four had ARF as a part multiple organ (MOF) septic origin, 2 isolated because primary disease. During study, all received defined total parenteral nutrition (TPN). The mean AA dialysate relatively low (0.61 ± 0.1 g N ) reached 4.5% substitution. Gln...
Context Hypothermia is known to influence thromboelastography (TEG). TEG reproducibility generally low. Objective The aim of this study was evaluate the rationale temperature adjustment in patients during hypothermia. We hypothesised that would not be important because low reproducibility. Design Prospective observational study. Setting Single-centre, secondary care performed 01/2009 07/2010. Patients Survivors cardiopulmonary resuscitation whom therapeutic hypothermia (32 34°C) indicated...