A5000051355- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Autoimmune and Inflammatory Disorders Research
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- Sepsis Diagnosis and Treatment
- Parvovirus B19 Infection Studies
- Viral Infections and Vectors
- Long-Term Effects of COVID-19
- Complement system in diseases
- Kawasaki Disease and Coronary Complications
- Inhalation and Respiratory Drug Delivery
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Mobile Health and mHealth Applications
- Pharmacovigilance and Adverse Drug Reactions
- Immune responses and vaccinations
- Vector-borne infectious diseases
- MicroRNA in disease regulation
- Cell Adhesion Molecules Research
University Hospital Ulm
2014-2024
Universität Ulm
2002-2018
University College Cork
2015
University of Freiburg
2014-2015
University and Rehabilitation Clinics Ulm
2004-2014
University of Applied Sciences Kaiserslautern
2011
Sepsis continues to be a major cause of death, disability, and health-care expenditure worldwide. Despite evidence suggesting that host genetics can influence sepsis outcomes, no specific loci have yet been convincingly replicated. The aim this study was identify genetic variants survival.
An improved enzyme-linked immunosorbent (ELISA) assay using one-step antibody immobilization has been developed for the detection of human fetuin A (HFA), a specific biomarker atherosclerosis and hepatocellular carcinoma. The anti-HFA formed stable complex with 3-aminopropyltriethoxysilane (APTES) by ionic hydrophobic interactions. adsorbed on microtiter plates exhibited range 4.9 pg mL−1 to 20 ng HFA, limit 7 mL−1. Furthermore, an analytical sensitivity 10 was achieved, representing 51-fold...
Patients suffering from chronic fatigue syndrome (CFS) or post-COVID (PCS) exhibit a reduced physiological performance capability. Impaired mitochondrial function and morphology may play pivotal role. Thus, we aimed to measure the muscle oxidative phosphorylation (OXPHOS) capacity assess in CFS PCS patients comparison healthy controls (HCs). Mitochondrial OXPHOS was measured permeabilized fibers using high-resolution respirometry. (subsarcolemmal/intermyofibrillar...
Infection with Helicobacter pylori, a Gram-negative bacterium, is strongly associated gastric ulcers and adenocarcinoma. The mechanisms by which the innate immune system recognizes H. pylori lipopolysaccharide (LPS) remain unclear. Contradictory reports exist that suggest Toll-like receptors are involved. In this study we evaluated interactions of LPS from different strains pylori. Using reporter cell lines, as well HEK293 cells transfected either CD14 TLR4, or TLR2, show LPS-induced...
A rapid and highly-sensitive surface plasmon resonance (SPR)-based immunoassay (IA) has been developed validated for detecting human C-reactive protein (CRP), a specific biomarker inflammatory metabolic disorders, infections. The 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC)-activated A/G (Pr A/G) was diluted in 1% (v/v) 3-aminopropyltriethoxysilane (APTES), dispensed on KOH-treated gold (Au)-coated SPR chip, incubated 30 min. Pr functionalized Au chip then bound to...
Virus-associated chronic inflammation may contribute to autoimmunity in a number of diseases. In the brain, autoimmune encephalitis appears related fluctuating reactivation states neurotropic viruses. addition, viral miRNAs and proteins can be transmitted via exosomes, which constitute novel but highly relevant mediators cellular communication. The current study questioned role HSV-1-encoded host-derived cerebrospinal fluid (CSF)-derived enriched from stress-induced neuroinflammatory...
X-linked osteopetrosis, anhydrotic ectodermal dysplasia, and immunodeficiency (XL-O-EDA-ID) is a disorder that caused by hypomorphic mutations in the nuclear factor kappaB essential modulator (NEMO). These lead to an impaired NF-kappaB activation. In vitro analyses studies animal models show inhibition of leads decrease cytokine production T-cell proliferation. Patients classically display poor or delayed inflammatory response infections. We describe boy with XL-O-EDA-ID, 1167-1168insC NEMO...
Abstract Both hemophagocytic lymphohistiocytosis (HLH) and Kawasaki disease (KD) are diagnosed in patients with prolonged resistant fever by using a scoring system. Concurrent manifestation of both conditions has been reported previously. We describe an infant 7 weeks whose condition fulfilled the criteria HLH, but who, after clinical response to treatment, suddenly died from myocardial infarction at 11 weeks. Post‐mortem examination revealed previously unknown coronary arteritis typical for...
In this article, we report on the electronic monitoring of DNA denaturation by NaOH using electrochemical impedance spectroscopy in combination with fluorescence imaging as a reference technique. The probe consisting 36-mer fragment was covalently immobilized nanocrystalline-diamond electrodes and hybridized different types 29-mer target (complementary, single-nucleotide defects at two positions, non-complementary random sequence). mathematical separation impedimetric signals into time...
This article describes a highly-sensitive surface plasmon resonance (SPR)-based immunoassay (IA) for human fetuin A (HFA), specific biomarker atherosclerosis and hepatocellular carcinoma. The assay is based on novel immobilization procedure that simply involves the dilution of an anti-HFA capture antibody (Ab) in 1% (v/v) 3-aminopropyltriethoxysilane (APTES), followed by its dispensing KOH-treated gold (Au)-coated SPR chip incubation 30 min. developed IA detected 0.3-20 ng mL(-1) HFA with...