A5068224616- Respiratory Support and Mechanisms
- Sepsis Diagnosis and Treatment
- Cardiac Arrest and Resuscitation
- Sulfur Compounds in Biology
- Neuroscience of respiration and sleep
- Mitochondrial Function and Pathology
- Renal function and acid-base balance
- Hemodynamic Monitoring and Therapy
- Intensive Care Unit Cognitive Disorders
- Thermal Regulation in Medicine
- Traumatic Brain Injury and Neurovascular Disturbances
- Genetics, Aging, and Longevity in Model Organisms
- Airway Management and Intubation Techniques
- Mechanical Circulatory Support Devices
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Respiratory Health Research
- Metabolism and Genetic Disorders
- Cardiac Ischemia and Reperfusion
- Alcohol Consumption and Health Effects
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Stress Responses and Cortisol
- Abdominal Surgery and Complications
- Erythropoietin and Anemia Treatment
- Acute Kidney Injury Research
- Pulmonary Hypertension Research and Treatments
Helmholtz-Institute Ulm
2009-2025
Universität Ulm
2014-2024
University Hospital Ulm
2015-2024
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2018
Deutsche Forschungsgemeinschaft
2016
Boehringer Ingelheim (Germany)
2015
Technische Hochschule Ulm
2012
University and Rehabilitation Clinics Ulm
2012
Zero to Three
2011
Université d'Angers
2011
Mitochondrial dysfunction might have a central role in the pathophysiology of depression. Phenotypically, depression is characterized by lack energy, concentration problems and fatigue. These symptoms be partially explained reduced availability adenosine triphosphate (ATP) as consequence impaired mitochondrial functioning. This study investigated respiration peripheral blood mononuclear cells (PBMCs), an established model to investigate was assessed intact PBMCs 22 individuals with diagnosis...
In awake spontaneously breathing mice, inhaling gaseous hydrogen sulfide (H2S) produced a "suspended animation-like" metabolic status with hypothermia and reduced O2 demand, thus protecting from lethal hypoxia. Murine models may be questioned, however, because due to their large surface area/mass ratio, rodents can rapidly drop core temperature. Therefore, we investigated whether intravenous H2S (Na2S, sodium sulfide) would induce comparable response in anesthetized mechanically ventilated...
Patients suffering from chronic fatigue syndrome (CFS) or post-COVID (PCS) exhibit a reduced physiological performance capability. Impaired mitochondrial function and morphology may play pivotal role. Thus, we aimed to measure the muscle oxidative phosphorylation (OXPHOS) capacity assess in CFS PCS patients comparison healthy controls (HCs). Mitochondrial OXPHOS was measured permeabilized fibers using high-resolution respirometry. (subsarcolemmal/intermyofibrillar...
Cellular responses to oxygen tension have been studied extensively. Oxygen can be determined by considering the phosphorescence lifetime of a sensor. The simultaneous usage FLIM coenzymes as NAD(P)H and FAD(+) PLIM sensors could provide information about correlation metabolic pathways tension. We investigated correlative NAD(P)H-FLIM sensing-PLIM for simultaneously analyzing cell metabolism Cell pO2 were observed under different hypoxic conditions in squamous carcinoma cultures complex ex...
Mammals display a wide range of variation in their lifespan. Investigating the molecular networks that distinguish long- from short-lived species has proven useful to identify determinants longevity. Here, we compared livers young and old long-lived naked mole-rats (NMRs) phylogenetically closely related, shorter-lived, guinea pigs using an integrated omics approach. We found NMR unique expression pattern mitochondrial proteins results distinct metabolic features mitochondria. For instance,...
Objective: Early goal-directed therapy aims at balancing tissue oxygen delivery and demand. Hyperoxia (i.e., pure breathing) has not been studied in this context, since sepsis increases radical production, which is believed to be directly related the tension. On other hand, breathing improved survival various shock models. Therefore, we hypothesized that hyperoxia may beneficial during early septic shock. Design: Laboratory animal experiments. Setting: Animal research laboratory university...
The current practice of mechanical ventilation comprises the use least inspiratory O2 fraction associated with an arterial tension 55 to 80 mm Hg or hemoglobin saturation 88% 95%. Early goal-directed therapy for septic shock, however, attempts balance delivery and demand by optimizing cardiac function concentration, without making hyperoxia. Clearly, it has been well-established more than a century that long-term exposure pure results in pulmonary and, under hyperbaric conditions, central...
Mitochondrial functions include production of energy, activation programmed cell death, and a number specific tasks, e.g., signaling, control Ca2+ metabolism, synthesis important biomolecules. As proper mitochondrial function is critical for normal performance survival cells, dysfunction often leads to pathological conditions resulting in various human diseases. Recently has been linked multiple organ failure (MOF) leading the death care patients. However, there are two main reasons why this...
In rodents, inhaled H2S and injection of donors protected against kidney ischemia/reperfusion (I/R) injury. During porcine aortic occlusion, the donor Na2S (sulfide) reduced energy expenditure decreased noradrenaline requirements needed to maintain hemodynamic targets during early reperfusion. Therefore, we tested hypothesis whether sulfide pretreatment may also ameliorate organ function in occlusion-induced I/R Anesthetized, ventilated, instrumented pigs randomly received either or vehicle...
Abstract Introduction Infusing arginine vasopressin (AVP) in vasodilatory shock usually decreases cardiac output and thus systemic oxygen transport. It is still a matter of debate whether this vasoconstriction impedes visceral organ blood flow thereby causes dysfunction injury. Therefore, we tested the hypothesis low-dose AVP safe with respect to liver, kidney, heart function injury during resuscitated septic shock. Methods After intraperitoneal inoculation autologous feces, 24 anesthetized,...
To test the hypothesis whether inhaled hydrogen sulfide amplifies effects of deliberate hypothermia during anesthesia and mechanical ventilation as is used to provide organ protection after brain trauma or circulatory arrest. Awake mice inhaling exhibit reduced energy expenditure, hypothermia, bradycardia despite unchanged systolic heart function. In rodents, alone causes decreased metabolic rate thus bradycardia.Prospective, controlled, randomized study.University animal research...
Inhaling hydrogen sulfide (H2S) reduced energy expenditure resulting in hypothermia. Because the inflammatory effects of either hypothermia alone or H2S per se still are a matter debate, we tested hypothesis whether inhaled amplifies hypothermia-related modulation response. Fifteen hours after cecal ligation and puncture sham laparotomy, anesthetized mechanically ventilated normothermic hypothermic mice (core temperature kept at 38°C 27°C, respectively) received 100 ppm vehicle. In...
Objective: Controversial data are available on the effects of hydrogen sulfide during hemorrhage. Because clinical significance administration in rodents may not be applicable to larger species, we tested hypothesis whether intravenous Na2S (sulfide) would beneficially influence organ dysfunction long-term, porcine hemorrhage and resuscitation. Design: Prospective, controlled, randomized study. Setting: University animal research laboratory. Subjects: Forty-five domestic pigs either gender....
Abstract Purpose Adrenomedullin (ADM) has been referred to as a double-edged sword during septic shock: On one hand, ADM supplementation improved organ perfusion and function, attenuated systemic inflammation, ultimately reduced tissue apoptosis mortality. the other overproduction can cause circulatory collapse failure due impaired vasoconstrictor response myocardial contractility. Since most of these data originate from un-resuscitated shock models, we tested hypothesis whether newly...
Hemorrhagic shock-induced tissue hypoxia induces hyperinflammation, ultimately causing multiple organ failure. Hyperoxia and hypothermia can attenuate due to increased oxygen supply decreased demand, respectively. Therefore, we tested the hypothesis whether mild therapeutic hyperoxia would postshock hyperinflammation thereby dysfunction.Prospective, controlled, randomized study.University animal research laboratory.Thirty-six Bretoncelles-Meishan-Willebrand pigs of either gender.After 4...
Alterations of cellular bioenergetics are a common feature in most neurodegenerative disorders. However, there is selective vulnerability different brain regions, cell types, and even mitochondrial populations to these metabolic disturbances. Thus, the aim our study was establish validate an vivo imaging technique screen for function on subcellular level. Based nicotinamide adenine dinucleotide (phosphate) fluorescence lifetime microscopy [NAD(P)H FLIM], we performed quantitative correlation...
Endonuclease G (EndoG) is a nuclear-encoded endonuclease, mostly localised in mitochondria. In the nucleus EndoG participates site-specific cleavage during replication stress and genome-wide DNA degradation apoptosis. However, impact of on mitochondrial (mtDNA) metabolism poorly understood. Here, we investigated whether involved regulation mtDNA removal aberrant copies. We applied single-cell Transcription Replication Imaging Protocol (mTRIP) PCR-based strategies human cells after...