A5089733480- Genetics, Aging, and Longevity in Model Organisms
- RNA and protein synthesis mechanisms
- Evolution and Paleontology Studies
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Physiological and biochemical adaptations
- Genetic Syndromes and Imprinting
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Bat Biology and Ecology Studies
- Molecular Biology Techniques and Applications
- Antimicrobial Peptides and Activities
- Thyroid Disorders and Treatments
- Fungal Infections and Studies
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Plant Disease Resistance and Genetics
- Advanced biosensing and bioanalysis techniques
- Genomics and Rare Diseases
- Vitamin D Research Studies
- Retinoids in leukemia and cellular processes
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2014-2023
Leibniz Institute for Zoo and Wildlife Research
2020
Leibniz Association
2010-2015
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2013
Warsaw University of Life Sciences
2010-2012
Sorbonne Université
2012
Schiller International University
2009
Max Delbrück Center
2004
Institute of Molecular Biotechnology
2003
Princeton University
2001
So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells generate a transcriptome-wide map TISs in human monocytic cell line. A neural network was trained on footprints observed at previously annotated AUG codons (TICs), and used for ab initio prediction 5062 transcripts with sufficient sequence coverage. Functional interpretation suggested 2994 novel...
Abstract Background Millions of humans and animals suffer from superficial infections caused by a group highly specialized filamentous fungi, the dermatophytes, which exclusively infect keratinized host structures. To provide broad insights into molecular basis pathogenicity-associated traits, we report first genome sequences two closely phylogenetically related Arthroderma benhamiae Trichophyton verrucosum , both induce inflammatory in humans. Results 97% 22.5 megabase A. T. are...
Alternative splicing (AS) is a cellular process that increases cell's coding capacity from limited set of genes.Although AS common in higher plants and animals, its prevalence other eukaryotes mostly unknown.In fungi the involvement gene expression effect on multi-cellularity virulence great medical economic interest.We present genome-wide comparative study 23 informative different taxa, based alignments public transcript sequences.Random sampling expressed sequence tags allows for robust...
The naked mole-rat (Heterocephalus glaber) is a long-lived, cancer resistant rodent and there great interest in identifying the adaptations responsible for these other of its unique traits. We employed RNA sequencing to compare liver gene expression profiles between mole-rats wild-derived mice. Our results indicate that genes associated with oxidoreduction mitochondria were expressed at higher relative levels mole-rats. largest effect nearly 300-fold epithelial cell adhesion molecule...
The annual fish Nothobranchius furzeri is the vertebrate with shortest known life span in captivity. Fish of GRZ strain live only three to four months under optimal laboratory conditions, show explosive growth, early sexual maturation and age-dependent physiological behavioral decline, express aging related biomarkers. Treatment resveratrol low temperature significantly extends maximum span. These features make N. a promising new model for age research. To contribute establishing as...
Abstract Naked mole‐rats ( Heterocephalus glaber ) can be extremely long‐lived and are resistant to cancer. Hence, they have been proposed as a model organism for delayed ageing. Adaptation constant hypoxic hypercapnic environment has suggested reason their apparent ability tolerate oxidative stress. Nevertheless, little is known about the natural habitat which species evolved. mole‐rat burrow environments were assessed in Ethiopia Kenya. Despite reported thermolability of naked mole‐rats,...
Mammals display a wide range of variation in their lifespan. Investigating the molecular networks that distinguish long- from short-lived species has proven useful to identify determinants longevity. Here, we compared livers young and old long-lived naked mole-rats (NMRs) phylogenetically closely related, shorter-lived, guinea pigs using an integrated omics approach. We found NMR unique expression pattern mitochondrial proteins results distinct metabolic features mitochondria. For instance,...
Many comparative genomics studies aim to find the genetic basis of species-specific phenotypic traits. A prevailing strategy is search genome-wide for genes that evolved under positive selection based on non-synonymous synonymous substitution ratio. However, incongruent results largely due high false rates indicate need standardization quality criteria and software tools. Main challenges are ortholog isoform assignment, sensitivity statistical models alignment errors imperative parallelize...
The genetics of lifespan determination is poorly understood. Most research has been done on short-lived animals and it unclear if these insights can be transferred to long-lived mammals like humans. Some African mole-rats (Bathyergidae) have life expectancies that are multiple times higher than similar sized phylogenetically closely related rodents. To gain new into genetic mechanisms determining mammalian lifespans, we obtained genomic transcriptomic data from 17 rodent species scanned...
Abstract Increasing evidence links metabolism, protein synthesis, and growth signaling to impairments in the function of hematopoietic stem progenitor cells (HSPCs) during aging. The Lin28b/Hmga2 pathway controls tissue development, postnatal downregulation this limits self-renewal adult vs fetal (HSCs). Igf2bp2 is an RNA binding downstream Lin28b/Hmga2, which regulates messenger stability translation. role HSC aging unknown. In study, analysis wild-type knockout mice showed that oxidative...
Advances in second-generation sequencing of RNA made a near-complete characterization transcriptomes affordable. However, the reconstruction full-length mRNAs via de novo RNA-seq assembly is still difficult due to complexity eukaryote with highly similar paralogs and multiple alternative splice variants. Here, we present FRAMA, genome-independent annotation tool for mRNA assemblies that addresses several post-assembly tasks, such as reduction contig redundancy, ortholog assignment,...
In the course of determining sequence Dictyostelium discoideum genome we have characterized in detail quantity and nature interspersed repetitive elements present this species. Several most abundant small complex repeats transposons (DIRS-1; TRE3-A,B; TRE5-A; skipper; Tdd-4; H3R) been described previously. our analysis identified additional elements. Thus, can now a complete list D. . All add up to 10% genome. Some newly belong established classes (TRE3-C, D; TRE5-B,C; DGLT-A,P; Tdd-5)....
One unexpected feature of the human genome is high structural variability across individuals. Frequently, large regions show polymorphisms and many vary in their abundance. However, accurate methods for characterization typing such copy number variations (CNV) are needed. The defensin cluster at region 8p23.1 one best studied CNV due to its potential clinical relevance innate immunity, inflammation, cancer. can be divided into two subclusters, which harbor predominantly either alpha- or...
Several recent studies indicate that alternative splicing in Arabidopsis and other plants is a common mechanism for post-transcriptional modulation of gene expression. However, few analyses have been done so far to elucidate the functional relevance higher plants. Representing frequent universal subtle event among eukaryotes, at NAGNAG acceptors contributes transcriptome diversity therefore, proteome plasticity. Alternatively spliced are overrepresented genes coding proteins with...
Sexual activity and/or reproduction are associated with a doubling of life expectancy in the long-lived rodent genus Fukomys. To investigate molecular mechanisms underlying this phenomenon, we analyzed 636 RNA-seq samples across 15 tissues. This analysis suggests that changes regulation hypothalamic-pituitary-adrenal stress axis play key role regarding extended reproductive vs. non-reproductive mole-rats. is substantiated by corpus independent evidence. In accordance previous studies,...
Abstract Background Defensins are important components of innate immunity to combat bacterial and viral infections, can even elicit antitumor responses. Clusters defensin (DEF) genes located in a 2 Mb range the human chromosome 8p23.1. This DEF locus, however, represents one regions euchromatic part final genome sequence which contains segmental duplications, recalcitrant gaps indicating high structural dynamics. Results We find that inter- intraindividual genetic variations within this...
Naked mole-rats (NMRs) are eusocially organized in colonies. Although breeders carry the additional metabolic load of reproduction, they extremely long-lived and remain fertile throughout their lifespan. This phenomenon contrasts disposable soma theory aging stating that organisms can invest resources either somatic maintenance, enabling a longer lifespan, or at cost longevity. Here, we present comparative transcriptome analysis vs. non-breeders eusocial, NMR polygynous shorter-lived guinea...
Ansell's mole-rats (Fukomys anselli) are subterranean, long-lived rodents, which live in eusocial families, where the maximum lifespan of breeders is twice as long that non-breeders. Their metabolic rate significantly lower than expected based on allometry, and their retinae show a high density S-cone opsins. Both features may indicate naturally low thyroid hormone levels. In present study, we sequenced several major components pathways analyzed free total thyroxine triiodothyronine serum...