Mario Brosch
A5066017161- Liver Disease Diagnosis and Treatment
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Liver physiology and pathology
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Diet, Metabolism, and Disease
- Alcohol Consumption and Health Effects
- Endoplasmic Reticulum Stress and Disease
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Genetics, Bioinformatics, and Biomedical Research
- RNA Research and Splicing
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics, phytochemicals, and oxidative stress
- Genetic factors in colorectal cancer
- Pediatric Hepatobiliary Diseases and Treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- CRISPR and Genetic Engineering
- Gallbladder and Bile Duct Disorders
- Pharmacogenetics and Drug Metabolism
- Genomics and Phylogenetic Studies
- Lipid metabolism and biosynthesis
Technische Universität Dresden
2016-2025
University Hospital Carl Gustav Carus
2017-2025
Heinrich Heine University Düsseldorf
2022
Düsseldorf University Hospital
2022
Klinik und Poliklinik für Psychotherapie und Psychosomatik
2018-2021
University Medical Center
2020
Berlin-Brandenburger Centrum für Regenerative Therapien
2019
Kiel University
2005-2014
University Hospital Schleswig-Holstein
2007-2013
University of Lübeck
2007-2013
Significance Because obese people are at an increased risk of many age-related diseases, it is a plausible hypothesis that obesity increases the biological age some tissues and cell types. However, has been difficult to detect such accelerated aging effect because unclear how measure tissue age. Here we use recently developed biomarker (known as “epigenetic clock”) study relationship between epigenetic in several human tissues. We report unexpectedly strong correlation high body mass index...
So far, the annotation of translation initiation sites (TISs) has been based mostly upon bioinformatics rather than experimental evidence. We adapted ribosomal footprinting to puromycin-treated cells generate a transcriptome-wide map TISs in human monocytic cell line. A neural network was trained on footprints observed at previously annotated AUG codons (TICs), and used for ab initio prediction 5062 transcripts with sufficient sequence coverage. Functional interpretation suggested 2994 novel...
Abstract Hepatic stellate cells (HSCs) have a central pathogenetic role in the development of liver fibrosis. However, their fibrosis-independent and homeostatic functions remain poorly understood 1–5 . Here we demonstrate that genetic depletion HSCs changes WNT activity zonation hepatocytes, leading to marked alterations regeneration, cytochrome P450 metabolism injury. We identify R-spondin 3 (RSPO3), an HSC-enriched modulator signalling, as responsible for these hepatocyte-regulatory...
Objective Homozygous alpha1-antitrypsin (AAT) deficiency increases the risk for developing cirrhosis, whereas relevance of heterozygous carriage remains unclear. Hence, we evaluated impact two most relevant AAT variants (‘Pi*Z’ and ‘Pi*S’), present in up to 10% Caucasians, on subjects with non-alcoholic fatty liver disease (NAFLD) or alcohol misuse. Design We analysed multicentric case–control cohorts consisting 1184 people biopsy-proven NAFLD 2462 chronic misuse, both comprising cases...
Diverticular disease is a common complex disorder characterised by mucosal outpouchings of the colonic wall that manifests through complications such as diverticulitis, perforation and bleeding. We report to date largest genome-wide association study (GWAS) identify genetic risk factors for diverticular disease.Discovery GWAS analysis was performed on UK Biobank imputed genotypes using 31 964 cases 419 135 controls European descent. Associations were replicated in sample 3893 2829...
The rs641738C>T variant located near the membrane-bound O-acyltransferase domain containing 7 (MBOAT7) locus is associated with fibrosis in liver diseases, including non-alcoholic fatty disease (NAFLD), alcohol-related disease, hepatitis B and C. We aim to understand mechanism by which contributes pathogenesis of NAFLD.
Objective The intestinal epithelium is a rapidly renewing tissue which plays central roles in nutrient uptake, barrier function and the prevention of inflammation. Control epithelial differentiation essential to these processes dependent on cell type-specific activity transcription factors bind accessible chromatin. Here, we studied role SET Domain Bifurcated Histone Lysine Methyltransferase 1, also known as ESET (SETDB1), histone H3K9 methyltransferase, homeostasis IBD. Design We...
Nonalcoholic fatty liver disease (NAFLD) is a common metabolic dysfunction leading to hepatic steatosis. However, NAFLD's global impact on the lipidome poorly understood. Using high-resolution shotgun mass spectrometry, we quantified molar abundance of 316 species from 22 major lipid classes in biopsies 365 patients, including nonsteatotic patients with normal or excessive weight, diagnosed NAFL (nonalcoholic liver) NASH steatohepatitis), and bearing mutations NAFLD-related protein factors....
Abstract The liver has the remarkable capacity to regenerate. In clinic, regeneration is induced by portal vein embolization, which redirects blood flow, resulting in hypertrophy locations with increased supply, and atrophy of embolized segments. Here, we apply single-cell single-nucleus transcriptomics on healthy, hypertrophied, atrophied patient-derived samples explore cell states regenerating liver. Our data unveils pervasive upregulation genes associated developmental processes, cellular...
Abberrant DNA methylation is one of the hallmarks cancerogenesis. Our study aims to delineate differential in cirrhosis and hepatic Patterns 27,578 individual CpG loci 12 hepatocellular carcinomas (HCCs), 15 cirrhotic controls normal liver samples were investigated using an array-based technology. A supervised principal component analysis (PCA) revealed 167 hypomethylated 100 hypermethylated HCC as compared controls. Thus, these show a "cirrhotic" pattern that maintained HCC. In pairwise...
The sterolin locus (ABCG5/ABCG8) confers susceptibility for cholesterol gallstone disease in humans. Both the responsible variant and molecular mechanism causing an increased incidence of gallstones these patients have as yet not been identified. Genetic mapping utilized patient samples from Germany (2,808 cases, 2,089 controls), Chile (680 442 Denmark (366 766 India (247 224 China (280 244 controls). Analysis allelic imbalance complementary DNA (cDNA) human liver (n = 22) was performed...
A deeper epigenomic understanding of spatial organization cells in human tissues is an important challenge. Here we report the first combined positional analysis transcriptomes and methylomes across three micro-dissected zones (pericentral, intermediate periportal) liver. We identify pronounced anti-correlated transcriptional methylation gradients including a core 271 genes controlling zonated metabolic morphogen networks observe prominent porto-central gradient DNA at binding sites 46...