A5087750544- Gut microbiota and health
- Hematopoietic Stem Cell Transplantation
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Childhood Cancer Survivors' Quality of Life
- Immune Cell Function and Interaction
- Bacterial Identification and Susceptibility Testing
- Tryptophan and brain disorders
- Immune Response and Inflammation
- Listeria monocytogenes in Food Safety
- Antibiotic Resistance in Bacteria
- Neutropenia and Cancer Infections
- Complementary and Alternative Medicine Studies
- COVID-19 Clinical Research Studies
- COVID-19 diagnosis using AI
- Oral microbiology and periodontitis research
- Mycobacterium research and diagnosis
- Infective Endocarditis Diagnosis and Management
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Renal Transplantation Outcomes and Treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Dysphagia Assessment and Management
Institute of Medical Microbiology and Hygiene
2015-2025
University Hospital Regensburg
2016-2025
University of Regensburg
2016-2024
Ljubljana University Medical Centre
2024
Instand
2024
Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of mammalian gut microbiomes. However, in absence a reference, this does not reveal alterations absolute abundance specific operational taxonomic units if microbial loads vary across specimens.Here we suggest spiking exogenous bacteria into crude specimens quantify ratios bacterial abundances. We use rDNA read counts spike-in adjust endogenous for changes total loads. Using series dilutions...
In allogeneic stem cell transplantation (ASCT), systemic broad-spectrum antibiotics are frequently used for treatment of infectious complications, but their effect on microbiota composition is still poorly understood. This retrospective analysis 621 patients who underwent ASCT at the University Medical Center Regensburg and Memorial Sloan Kettering Cancer in New York assessed impact timing peritransplant antibiotic intestinal as well transplant-related mortality (TRM) overall survival. Early...
The composition of human as well animal microbiota has increasingly gained in interest since metabolites and structural components endogenous microorganisms fundamentally influence all aspects host physiology. Since many the bacteria are still unculturable, molecular techniques such high-throughput sequencing have dramatically increased our knowledge microbial communities. majority microbiome studies published thus far based on bacterial 16S ribosomal RNA (rRNA) gene sequencing, so that they...
Major depressive disorder is the main cause of disability worldwide with imperfect treatment options. However, novel therapeutic approaches are currently discussed, from augmentation strategies to treatments targeting immune system or microbiome-gut-brain axis. Therefore, we examined potential beneficial effects minocycline, a tetracycline antibiotic pleiotropic, immunomodulatory action, alone as escitalopram on behavior, prefrontal microglial density, and gut microbiome in rats selectively...
Maintaining gastrointestinal (GI) microbiome diversity plays a key role during allogeneic stem cell transplantation (ASCT), and loss of correlates with acute GI graft versus host disease (GvHD) poor outcomes.In this retrospective analysis 161 ASCT patients, we used serial analyses urinary 3-indoxyl sulfate (3-IS) levels parameters within the first 10 days after to identify potential commensal microbiota-sparing antibiotics. Based on antibiotic activity, formed 3 subgroups (Rifaximin without...
Multidrug-resistant bacterial pathogens like vancomycin-resistant Enterococcus faecium (VREfm) are a critical threat to human health1. Daptomycin is last-resort antibiotic for VREfm infections with novel mode of action2, but which resistance has been widely reported unexplained. Here we show that rifaximin, an unrelated used prophylactically prevent hepatic encephalopathy in patients liver disease3, causes cross-resistance daptomycin VREfm. Amino acid changes arising within the RNA...
Antiseptics such as chlorhexidine digluconate (CHX) are widely used in clinical dental practice, but their potential risks, particularly regarding antimicrobial resistance (AMR), not yet known. This study explores the genomic and transcriptomic mechanisms of CHX adaptation 3 isolates Streptococcus spp. adapted counterparts. The analysis revealed mutations genes related to membrane structure, DNA repair, metabolic processes. Mutations include those diacylglycerol kinase that occurred...
Abstract CD83 is a maturation marker for dendritic cells. In the B cell lineage, expressed especially on activated cells and light zone during germinal center (GC) reaction. The function of GC responses unclear. CD83−/− mice have strong reduction CD4+ T cells, which makes it difficult to analyze functional role responses. Therefore, in present study we generated cell–specific conditional knockout (CD83 B-cKO) model. B-cKO show defective upregulation MHC class II CD86 expression impaired...
The use of NGS-based testing the bacterial microbiota is often impeded by inconsistent or non-reproducible results, especially when applying different analysis pipelines and reference databases. We investigated five frequently used software packages submitting same monobacterial datasets to them, representing V1-2 V3-4 regions 16S-rRNA gene 26 well characterized strains, which were sequenced Ion Torrent™ GeneStudio S5 system. results obtained divergent calculations relative abundance did not...
Acute graft-versus-host disease (aGvHD) is a significant complication after allogeneic hematopoietic stem cell transplantation (aHSCT), but major factors determining severity are not well defined yet. By combining multiplexed tissue imaging and single-cell RNA sequencing on gastrointestinal biopsies from aHSCT-treated individuals with fecal microbiome analysis, we link high diversity the abundance of short-chain fatty acid-producing bacteria to sustenance suppressive regulatory T cells...
Abstract Objectives The aims of this study were to investigate the antimicrobial efficacy antiseptics in saliva-derived microcosm biofilms, and examine phenotypic adaption bacteria upon repeated exposure sub-inhibitory antiseptic concentrations. Methods Saliva-derived biofilms formed mimicking caries- or gingivitis-associated conditions, respectively. Microbial compositions analyzed by semiconductor-based 16S rRNA sequencing. Biofilms treated with CHX, CPC, BAC, ALX, DQC for 1 10 min, colony...
In XIAP deficiency, ileitis is driven by TNFR1- and TNFR2-dependent targeting of TLR5-expressing Paneth dendritic cells.
Butyrogenic bacteria play an important role in gut microbiome homeostasis and intestinal epithelial integrity. Previous studies have demonstrated association between administration of short-chain fatty acids like butyrate protection from acute graft-vs-host disease (GvHD) after allogeneic stem cell transplantation (ASCT).In the current study, we examined abundance butyrogenic capacity butyrate-producing 28 healthy donors 201 patients ASCT. We prospectively collected serial stool samples...
Abstract Background At the entry site of respiratory virus infections, oropharyngeal microbiome has been proposed as a major hub integrating viral and host immune signals. Early studies suggested that infections with severe acute syndrome coronavirus 2 (SARS-CoV-2) are associated changes upper lower airway microbiome, specific microbial signatures may predict disease 2019 (COVID-19) illness. However, results not conclusive, critical illness can drastically alter patient’s through multiple...
Antiseptics are widely used in dental practice and included numerous over-the-counter oral care products. However, the effects of routine antiseptic use on microbial composition biofilms emergence resistant phenotypes remain unclear. Microcosm were inoculated from saliva samples four donors cultured Amsterdam Active Attachment biofilm model for 3 days. Then, they treated two times daily with chlorhexidine digluconate (CHX) or cetylpyridinium chloride (CPC) a period 7 Ecological changes upon...
Class 1 and 2 monoclonal antibodies inhibit SARS-CoV-2 entry by blocking the interaction of viral receptor-binding domain with angiotensin-converting enzyme (ACE2), while class 3 target a highly conserved epitope outside ACE2 binding site. We aimed to investigate plasticity spike protein propagating wild-type in presence antibody S309. After 12 weeks, we obtained strain that was completely resistant inhibition S309, due successively evolving amino acid exchanges R346S P337L located paratope...
In biomedicine, every molecular measurement is relative to a reference point, like fixed aliquot of RNA extracted from tissue, defined number blood cells, or volume biofluid. Reference points are often chosen for practical reasons. For example, we might want assess the metabolome diseased organ but can only measure metabolites in urine. this case, observable data indirectly reflects disease state. The statistical implications these discrepancies have not yet been discussed.Here, show that...
Stroke-associated pneumonia is a major cause for poor outcomes in the post-acute phase after stroke. Several studies have suggested potential links between neglected oral health and pneumonia. Therefore, aim of this prospective observational study was to investigate microbiota incidence patients consecutively admitted stroke unit with stroke-like symptoms. This involved three investigation timepoints. The baseline (within 24 h admission) collection demographic, neurological, immunological...