A5013889951- Hematopoietic Stem Cell Transplantation
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Renal and related cancers
- T-cell and B-cell Immunology
- Birth, Development, and Health
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
University of Southern California
2021-2023
Abstract Increasing evidence links metabolism, protein synthesis, and growth signaling to impairments in the function of hematopoietic stem progenitor cells (HSPCs) during aging. The Lin28b/Hmga2 pathway controls tissue development, postnatal downregulation this limits self-renewal adult vs fetal (HSCs). Igf2bp2 is an RNA binding downstream Lin28b/Hmga2, which regulates messenger stability translation. role HSC aging unknown. In study, analysis wild-type knockout mice showed that oxidative...
Cells do not make fate decisions independently. Arguably, every cell-fate decision occurs in response to environmental signals. In many cases, cell-cell communication alters the dynamics of internal gene regulatory network a cell initiate transitions, yet models rarely take this into account. Here, we have developed multiscale perspective study granulocyte-monocyte versus megakaryocyte-erythrocyte decisions. This transition is dictated by GATA1-PU.1 network: classical example bistable...
Inference of gene regulatory networks (GRNs) can reveal cell state transitions from single-cell genomics data. However, obstacles to temporal inference snapshot data are difficult overcome. Single-nuclei multiomics offer means bridge this gap and derive information using joint measurements expression chromatin accessibility in the same single cells. We developed popInfer infer that characterize lineage-specific dynamic Benchmarking against alternative methods for GRN inference, we showed...
Abstract Mammalian organs exhibit distinct physiology, disease susceptibility and injury responses between the sexes. In mouse kidney, sexually dimorphic gene activity maps predominantly to proximal tubule (PT) segments. Bulk RNA-seq data demonstrated sex differences were established from 4 8 weeks after birth under gonadal control. Hormone injection studies genetic removal of androgen estrogen receptors receptor (AR) mediated regulation in PT cells as regulatory mechanism. Interestingly,...