A5085144457- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Nanowire Synthesis and Applications
- Silicon Nanostructures and Photoluminescence
- Tissue Engineering and Regenerative Medicine
- Anodic Oxide Films and Nanostructures
- Immunotherapy and Immune Responses
- Graphene and Nanomaterials Applications
- Mesenchymal stem cell research
- Neuroscience and Neural Engineering
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Venous Thromboembolism Diagnosis and Management
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Quantum Dots Synthesis And Properties
- Genetic diversity and population structure
- Central Venous Catheters and Hemodialysis
- Bone Tissue Engineering Materials
- Atherosclerosis and Cardiovascular Diseases
- Nanoparticles: synthesis and applications
- Lung Cancer Research Studies
Houston Methodist
2013-2025
Methodist Hospital
2019-2025
Biodynamic Research Corporation (United States)
2022-2024
Indiana University – Purdue University Indianapolis
2024
Indiana University School of Medicine
2024
University of Southern California
2023
University of Miami
2022
Methodist Hospital
2011-2020
Ben-Gurion University of the Negev
2020
Methodist Sports Medicine
2019
Nanoneedles display potential in mediating the delivery of drugs and biologicals, as well intracellular sensing single-cell stimulation, through direct access to cell cytoplasm. enable cytosolic delivery, negotiating membrane endolysosomal system, thus overcoming these major obstacles efficacy nanotherapeutics. The low toxicity minimal invasiveness nanoneedles have a for sustained nonimmunogenic payloads vivo, provided that development biocompatible with simple deployment strategy is...
Through localized delivery of rapamycin via a biomimetic drug system, it is possible to reduce vascular inflammation and thus the progression disease.Use nanoparticles deliver vessel wall in an vivo model atherosclerosis after short dosing schedule.Biomimetic (leukosomes) were synthesized using membrane proteins purified from activated J774 macrophages. Rapamycin-loaded characterized dynamic light scattering found have diameter 108±2.3 nm, surface charge -15.4±14.4 mV, polydispersity index...
Tumor extracellular matrix (ECM) represents a major obstacle to the diffusion of therapeutics and drug delivery systems in cancer parenchyma. This biological barrier limits efficacy promising therapeutic approaches including siRNA or agents intended for thermoablation. After extravasation due enhanced penetration retention effect tumor vasculature, typical nanotherapeutics are unable reach nonvascularized anoxic regions deep within Here, we developed simple method provide mesoporous silica...
The advancement of nanotechnology toward more sophisticated bioinspired approaches has highlighted the gap between advantages biomimetic and biohybrid platforms availability manufacturing processes to scale up their production. Though transferring biological features from cells synthetic nanoparticles for drug delivery purposes have recently been reported, a standardizable, batch-to-batch consistent, scalable, high-throughput assembly method is required further develop these platforms....
Activation of the vascular endothelium is characterized by increased expression adhesion molecules and chemokines.This activation occurs early in progression several diseases triggers recruitment leukocytes.Inspired tropism leukocytes, we investigated leukocyte-based biomimetic nanoparticles (i.e., leukosomes) as a novel theranostic platform for inflammatory diseases.Methods: Leukosomes were assembled combining phospholipids membrane proteins from leukocytes.For imaging applications,...
Biomimetic nanovesicles deriving from leukocytes membrane proteins, called leukosomes, exhibit increased targeting of cancer vasculature and stroma by exploiting the inflammatory pathway responsible for recruiting immune cells to lesion.
A major challenge in regenerative medicine is to improve therapeutic cells' delivery and targeting using an efficient simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due their powerful immunosoppressive potential. Here we report a versatile method transiently overexpress hyaluronic acid (HA) receptor, CD44, on MSC membranes, homing potential towards inflammatory site without affecting behavior. The effect HA-coatings...
Here, we have demonstrated that biomimetic nanovesicles assembled from macrophages' membrane proteins are similar to macrophage-derived exosomes. The anti-inflammatory activity observed <italic>in vivo</italic> derives their direct interaction with macrophages.
Abstract In the field of oncology research, a deeper understanding tumor biology has shed light on role environmental conditions surrounding cancer cells. this regard, targeting microenvironment recently emerged as new way to access disease. work, novel extracellular matrix (ECM)-targeting nanotherapeutic was engineered using lipid-based nanoparticle chemically linked an inhibitor ECM-related enzyme, lysyl oxidase 1 (LOX), that inhibits crosslinking elastin and collagen fibers. We...
In the development of new nanoparticle-based technologies for therapeutic and diagnostic purposes, understanding fate nanoparticles in body is crucial. We recently developed a multistage vector delivery system comprising biodegradable biocompatible nanoporous silicon particles (first-stage microparticles [S1MPs]) able to host, protect, deliver second-stage (S2NPs) on intravenous injection. This aims at sequentially overcoming biologic barriers en route target site by separating assigning...
Approaches to achieve site-specific and targeted delivery that provide an effective solution reduce adverse, off target side effects are urgently needed for cancer therapy. Here, we utilized a Trojan-horse-like strategy carry photosensitizer Chlorin e6 conjugated porous silicon multistage nanovectors with tumor homing mesenchymal stem cells photodynamic therapy diagnosis. The inherent versatility of permitted the conjugation photosensitizers enable precise cell death induction (60%) upon...
Recently, engineering the surface of nanotherapeutics with biologics to provide them superior biocompatibility and targeting towards pathological tissues has gained significant popularity. Although functionalization drug delivery vectors cellular materials been shown synthetic particles unique biological properties, these approaches may have undesirable immunological repercussions upon systemic administration. Herein, we comparatively analyzed unmodified multistage nanovectors functionalized...
Abstract The characterization of nanomaterials and their influence on interactions with the biology cells tissues are still partially unknown. Multistage nanovectors based mesoporous silicon have been extensively studied for drug delivery, thermal heating, improved diagnostic imaging. Here, short‐ long‐term changes occurring in human upon internalization (MSV) analyzed. Using qualitative quantitative techniques as well vitro vivo biochemical, cellular, functional assays, it is demonstrated...
The ideal scaffold for regenerative medicine should concurrently mimic the structure of original tissue from nano‐ up to macroscale and recapitulate biochemical composition extracellular matrix (ECM) in space time. In this study, a multiscale approach is followed selectively integrate different types nanostructured composite microspheres loaded with reporter proteins, multi‐compartment collagen scaffold. Through preservation structural cues functionalized at microscale, its macroscopic...
Significance This study validates a path toward personalized medicine that utilizes survey of the effective signaling state tumors via posttranslational modification analysis as diagnostic alternative to direct examination genetic mutations. Here, this approach is utilized identify biomarkers for Cdk5-driven tumors. The same could be adapted markers other tumor drivers. As number cancer types in which Cdk5 implicated rising and drugs with potential selectively target are moved clinic,...
Abstract Small alterations during the early stages of innate immune response to an implant can drive large changes in adaptive immunity. Biomaterials for regenerative purposes be engineered modulate this beneficial ways. This study presents innovative patch designed and functionalized target immunity at site. Mannose moieties are incorporated into collagen patches, resulting a technology called Local Immunotuning Patch (LIP), directly interact with antigen presenting cells through their...