A5053869253- Sepsis Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Pulmonary Hypertension Research and Treatments
- Inflammation biomarkers and pathways
- Cardiac, Anesthesia and Surgical Outcomes
- Immune cells in cancer
- Intensive Care Unit Cognitive Disorders
- Immune responses and vaccinations
- Neuroscience of respiration and sleep
- Acute Myocardial Infarction Research
- Education, Psychology, and Social Research
- Immune Response and Inflammation
- Long-Term Effects of COVID-19
- Hemodynamic Monitoring and Therapy
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Aortic aneurysm repair treatments
- Acute Kidney Injury Research
- Cardiac Arrest and Resuscitation
- Traumatic Ocular and Foreign Body Injuries
- Vitamin C and Antioxidants Research
- Adenosine and Purinergic Signaling
- Coronary Artery Anomalies
- Restraint-Related Deaths
- Clusterin in disease pathology
- Operations Management Techniques
Masaryk University
2014-2024
St. Anne's University Hospital Brno
2011-2024
University Hospital Brno
2011-2024
International Clinical Research Center, St. Anne's University Hospital Brno
2014-2024
Mayo Clinic in Arizona
2020
Mayo Clinic
2014
Introduction Major clinically relevant inflammatory events such as septic shock and severe COVID-19 trigger dynamic changes in the host immune system, presenting promising candidates for new biomarkers to improve precision diagnostics patient stratification. Hepcidin, a master regulator of iron metabolism, has been intensively studied many pathologies associated with system activation, however these data have never compared other clinical settings. Thus, we aimed reveal dynamics regulation...
Abstract Around half of people with severe COVID‐19 requiring intensive care unit (ICU) treatment will survive, but it is unclear how the immune response to SARS‐CoV‐2 differs between ICU patients that recover and those do not. We conducted whole‐blood immunophenotyping upon admission during their uncovered marked differences in circulating cell subsets. At admission, who later succumbed had significantly lower frequencies all memory CD8+ T subsets, resulting increased CD4‐to‐CD8...
Abstract Sepsis is characterized by dynamic changes of the immune system resulting in deregulated inflammation and failure homoeostasis can escalate to septic shock. Circulating monocytes other innate cells are among first ones recognize clear pathogens. Monocytes have an important role sepsis shock been studied as potential diagnostic markers. In total, forty‐two patients with were recruited blood samples obtained within 12 hours ICU admission. We showed that frequency classical...
Septic shock is a major cause of mortality in ICU patients, its pathophysiology complex and not properly understood. Oxidative stress seems to be one the most important mechanisms progression multiple organ failure. In present pilot study, we have analysed eight oxidative-stress-related biomarkers seven consecutive time points (i.e., first days) 21 septic patients admitted ICU. Our objective was describe kinetics four related pro-oxidative processes (nitrite/nitrate, malondialdehyde,...
Background Hypoxia effects on pulmonary artery structure and function are key to diseases such as hypertension. Recent studies suggest that growth factors called neurotrophins, particularly brain-derived neurotrophic factor (BDNF), can influence lung function, their role in the warrants further investigation. In this study, we examined effect of hypoxia BDNF humans, hypoxia-enhanced expression signaling human smooth muscle cells (PASMCs). Methods Results 48h 1% enhanced TrkB expression, well...
Sepsis is a clinical syndrome characterized by dysregulated response to infection. It represents leading cause of mortality in ICU patients worldwide. Although sepsis the point interest research for several decades, its management and patient survival are improving slowly. Monitoring biomarkers their combinations could help early diagnosis, estimation prognosis patient's stratification treatment. Circulating soluble endoglin (sEng) cleaved extracellular part transmembrane glycoprotein...
Myeloid-derived suppressor cells (MDSCs) are important regulators of immune processes during sepsis in mice. However, confirming these observations humans has been challenging due to the lack defined preparation protocols and phenotyping schemes for MDSC subsets. Thus, it remains unclear how MDSCs involved acute whether they have a role long-term complications seen survivors. Here, we combined comprehensive flow cytometry with unsupervised clustering using self-organizing maps identify three...
COVID-19 manifestation is associated with a strong immune system activation leading to inflammation and subsequently affecting the cardiovascular system. The objective of study was reveal possible interconnection between prolongated development or exacerbation long-term complications after COVID-19. We investigated correlations humoral cellular markers together inflammation/dysfunction during onset subsequent recovery. analyzed 22 hospitalized patients severe within three timepoints (acute,...
<b><i>Background/Aims:</i></b> The pathophysiology of acute kidney injury (AKI) in ST-elevation myocardial infarction (STEMI) patients remains poorly explored. involvement the nitric oxide (NO) pathway has been demonstrated experimental ischemic AKI. aim this study was to assess predictive value circulating biomarkers NO for AKI STEMI patients. <b><i>Methods:</i></b> Four hundred and twenty-seven treated with primary percutaneous coronary...
Acute kidney injury (AKI) is associated with impaired outcomes in critically ill COVID-19 patients. However, the prognostic significance of early AKI poorly described. We aimed to determine whether on admission intensive care unit (ICU) and its development within first 48 h predict need for renal replacement therapy (RRT) increased mortality. An analysis 372 patients pneumonia requiring mechanical ventilation without advanced chronic disease from 2020 2021 was performed. The stages ICU Day 2...
Preterm infants can develop airway hyperreactivity and impaired bronchodilation following supplemental O2 (hyperoxia) in early life, making it important to understand mechanisms of hyperoxia effects. Endogenous hydrogen sulfide (H2 S) has anti-inflammatory vasodilatory effects with oxidative stress. There is little understanding H2 S signaling developing airways. We hypothesized that the endogenous system detrimentally influenced by conversely pathways be leveraged attenuate deleterious ....
Sepsis and septic shock remain leading causes of morbidity mortality for patients in the intensive care unit. During early phase, immune cells produce various cytokines to prompt activation system. Polymorphonuclear leukocytes (PMNs) respond different signals producing inflammatory factors executing their antimicrobial mechanisms, resulting engulfment elimination invading pathogens. However, excessive caused by produced during sepsis progression can lead alteration PMN signaling subsequent...
Our goal was to evaluate the effect of hyperoxia on sublingual and ileostomal microcirculation during hemorrhagic reperfusion shock in a porcine model simulating rupture an abdominal aortic aneurysm (AAA). We wanted test these two vascular beds because is known cause different arteriolar responses [1].
Background: Several years after the COVID–19 pandemic, role of trained immunity in remains controversial, and questions regarding long–term effects on immune cells remain unresolved. We investigated roles Bacillus Calmette–Guérin (BCG) vaccination latent infections progression sepsis. Methods: conducted a prospective analysis 97 individuals recovering from mild–to–critical 64 sepsis patients. Immune cell frequencies, expression functional markers, plasma titers anti–Toxoplasma...
Sepsis is a life-threatening condition characterised by an overwhelming immune response and high fatality. While most research has focused on its acute phase, many sepsis survivors remain immunologically weakened leaving them susceptible to serious complications from even mild infections. The mechanisms underlying this prolonged dysregulation unclear, limiting effective interventions. Here, we analysed whether induced long-term ″training″ in hematopoietic stem progenitor cells (HSPCs),...
Pneumatic weapons rarely cause severe trauma. However, pellet embolisation can and unexpected injuries.This is the case study of a 32 year old man, who was shot in chest with pneumatic rifle. Initially, urgent damage control surgery performed to resolve pneumothorax pericardial tamponade, but no projectile found. Subsequent atypical symptomatology led more extensive imaging that found embolised into right carotid artery, thrombosis middle cerebral development large hemispheric ischaemic...
Kontext: Cerebrální oxymetrie (rSO2) se jeví jako slibná metoda pro sledování adekvátnosti dodávky kyslíku do mozku v průběhu karotické endarterektomie (CEA). Cílem studie bylo zhodnotit vztah mezi závažností stenózy kontralaterální (neoperované) karotidy a změnami rSO2 CEA. Metodika: byla sledována u 38 pacientů podstupujících CEA regionální anestezii. Pacienti byli následně rozděleni podle přítomnosti (Gdef) nebo absence (Gnodef) nového neurologického deficitu. Výchozí před naložením...
During the first wave of SARS Cov-2 virus pandemic, we faced a rapidly spreading infection in Czech Republic. The lack experience with pandemic such magnitude and inconsistent information on situation China Italy meant that were forced to introduce an effective anti-epidemic measures very short period protect our patients. One key significantly contributed successful management was prevent spread among healthcare professionals who directly cared for patients active infection. after conducted...