A5037697352- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Protein purification and stability
- Biochemical and Structural Characterization
- Glycosylation and Glycoproteins Research
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Virology and Viral Diseases
- Microbial Metabolic Engineering and Bioproduction
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Probiotics and Fermented Foods
- Genetics, Bioinformatics, and Biomedical Research
- Bacterial Identification and Susceptibility Testing
- Gene Regulatory Network Analysis
- Transgenic Plants and Applications
- Dupuytren's Contracture and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Biosensing Techniques and Applications
- Carbohydrate Chemistry and Synthesis
MB Pharma (Czechia)
2018-2025
Masaryk University
2016-2024
Bacteriophages from the family Myoviridae use double-layered contractile tails to infect bacteria. Contraction of tail sheath enables tube penetrate through bacterial cell wall and serve as a channel for transport phage genome into cytoplasm. However, mechanisms controlling contraction release phages with "double-layered" baseplates were unknown. We used cryo-electron microscopy show that binding Twort-like phi812 Staphylococcus aureus requires 210° rotation heterohexameric receptor-binding...
Abstract To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding adaptation processes primed by metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize non-native sugar D-xylose, but its assimilation into bacterial biochemical network via exogenous xylose...
Abstract Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages increase the effectivity of phage preparations emerging phage-resistant strains. Here we described genomic diversity spontaneous host-range mutants kayvirus 812. Five mutant were isolated as rare plaques on Staphylococcus aureus The host range 812-derived was 42% higher than wild type, determined a set 186 methicillin-resistant S. strains representing...
Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that notable for its ability to form biofilm and high rates of antibiotic resistance. It serves as reservoir multiple antimicrobial resistance genes spread among the staphylococcal population by horizontal gene transfer such transduction. While phage-mediated transduction well studied in aureus, S. transducing phages have not been described detail yet. Here, we report characteristics four phages, 27,...
The preparation of a solid dosage form containing bacteriophages, which meets pharmaceutical requirements and ensures long-term stability the phage effect, is significant for implementing therapy in practice. A commonly used method processing phages into freeze-drying (so-called) freeze-dried cake; however, to date there have been no studies examining pharmacopeial parameters tablets with bacteriophages. In this study, we describe properties cocktail purified pseudomonal bacteriophage DSM...
Escherichia coli phage SU10 belongs to the genus Kuravirus from class Caudoviricetes of phages with short non-contractile tails. In contrast other short-tailed phages, tails Kuraviruses elongate upon cell attachment. Here we show that virion has a prolate head, containing genome and ejection proteins, tail, which is formed portal, adaptor, nozzle, tail needle proteins decorated long fibers. The binding fibers receptors in outer bacterial membrane induces straightening nozzle rotation After...
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as significant threat. Phage therapy could offer safe and effective alternative to antibiotics. preparations should comply quality safety requirements; therefore, it important develop efficient production control technologies. This study was conducted evaluate rapid reliable method for identifying staphylococcal bacteriophages, based on...
Phages with contractile tails employ elaborate strategies to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Most tailed phages that have been structurally characterized date infect Gram-negative bacteria, but those targeting Gram-positive many of which are important human pathogens, less well understood. Here, we show baseplate phage phi812, infects Staphylococcus aureus , is formed a core, wedge modules, arms carrying receptor-binding proteins type 1 2...
Clostridium histolyticum is used for production of several proteolytic enzymes such as elastase, neutral proteases, clostripain and in particular collagenase. Besides industrial applications, collagenase has been indispensable medical purposes including isolation pancreatic islets diabetes treatment. The aim this study was to optimize the method partial purification a new blend test its suitability successful rat model. Bacterial strain C. sequenced presence genes. Different fermentation...
Clostridial collagenases are essential biotechnological tissue dissociation agents owing to their ability cleave different types of collagen. Standardization collagenase-based protocols has been hampered by impurities in products manufactured from Clostridium histolyticum. To enhance the purification process, we produced recombinant collagenase classes G and H, taking advantage Escherichia coli expression system. The respective gene sequences were derived C. histolyticum modified addition a...
Abstract D-Xylose, a major constituent of plant biomass and second most abundant sugar on Earth, holds considerable potential as substrate for sustainable bio-production. Pseudomonas putida KT2440 is an attractive bacterial host valorizing biogenic feedstocks but lacks xylose utilization pathway. While several attempts to engineer P. growth have been reported, comprehensive understanding metabolism in this bacterium lacking, hindering its further improvement rational tailoring specific...