A5033154839- Plant nutrient uptake and metabolism
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Plant Reproductive Biology
- Skin and Cellular Biology Research
- Synthesis and Characterization of Heterocyclic Compounds
- Photosynthetic Processes and Mechanisms
- Synthesis of Tetrazole Derivatives
- Quinazolinone synthesis and applications
- Polysaccharides and Plant Cell Walls
- Vector-borne infectious diseases
- Biochemical and Structural Characterization
- Parasite Biology and Host Interactions
- Plant Stress Responses and Tolerance
- Synthesis and Biological Evaluation
- Glycosylation and Glycoproteins Research
- Fungal and yeast genetics research
- 14-3-3 protein interactions
- Toxin Mechanisms and Immunotoxins
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Fluorine in Organic Chemistry
- Synthesis and Reactions of Organic Compounds
Veterinary Research Institute
2020-2024
Central European Institute of Technology – Masaryk University
2011-2017
Masaryk University
2006-2017
Central European Institute of Technology
2011-2017
Central European Institute of Technology
2017
University of Vienna
2001-2013
Max Perutz Labs
2004-2013
Mendel University in Brno
2008-2011
Czech Academy of Sciences, Institute of Biophysics
2011
MRC Laboratory of Molecular Biology
2008
Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via phosphotransfer proteins (AHP1-AHP5) nuclear response regulators. contrast ancestral two-component bacteria, protein interactions plant MSP are supposed be rather nonspecific. Here, we show that C-terminal receiver domain HK CKI1 (CKI1(RD) ) responsible for recognition downstream partners, and specifically...
The transition of microtubules (MTs) from an assembled to a disassembled state plays essential role in several cellular functions. While MT dynamics are often linked those actin filaments, little is known about whether intermediate filaments (IFs) have influence on dynamics. We show here that plectin 1c (P1c), one the multiple isoforms IF-associated cytolinker protein plectin, acts as destabilizer. found MTs P1c-deficient (P1c(-/-)) keratinocytes more resistant toward nocodazole-induced...
Plectin, a large and widely expressed cytolinker protein, is composed of several subdomains that harbor binding sites for variety different interaction partners. A canonical actin‐binding domain (ABD) comprising two calponin homology domains (CH1 CH2) located in proximity to its amino terminus. However, the ABD plectin unique among proteins as it form distinct, isoform‐specific versions. We have determined three‐dimensional structure distinct crystalline forms one versions (pleABD/2α) from...
Summary The diversity of 103 clinical isolates the Acinetobacter calcoaceticus–Acinetobacter baumannii complex obtained between 1991 and 1997 from 17 Czech hospitals was studied by ribotyping, biotyping, plasmid profiling antibiotic susceptibility testing. According to EcoRI ribotypes, all but one these were identified DNA group level: 77 allocated 2 (A. baumannii), 14 3, 10 13 sensu Tjernberg Ursing 1 calcoaceticus). In total, 50 different ribotypes biotypes observed. Plasmids found in 92%...
The gene pkwA coding for a typical WD-repeat protein was found in the chromosome of bacterium Thermomonospora curvata CCM 3352. Until now proteins were through to be confined eukaryotes.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFurther studies on the cyclization of aromatic azomethines ortho-substituted with a trifluoromethyl group: synthesis 2,4-di- or 2,3,4-trisubstituted quinolinesLucjan Strekowski, Steven E. Patterson, Lubomir Janda, Roman L. Wydra, Donald B. Harden, Malgorzata Lipowska, and Marek T. CeglaCite this: J. Org. Chem. 1992, 57, 1, 196–201Publication Date (Print):January 1992Publication History Published online1 May 2002Published inissue 1 January...
As an intermediate filament (IF)-based cytolinker protein, plectin plays a key role in the maintenance of cellular cytoarchitecture and serves at same time as scaffolding platform for signaling cascades. Consisting six structural repeats (R1-6) harboring binding sites different IF proteins involved signaling, C-terminal domain is strategic functional importance. Depending on species, it contains least 13 cysteines, 4 which reside R5 domain. To investigate biological functions we used...
Parasite inhibitors of cysteine peptidases are known to influence a vast range processes linked degradation either the parasites' own proteins or native their hosts. We characterise novel type I cystatin (stefin) found in sanguinivorous fish parasite Eudiplozoon nipponicum (Platyhelminthes: Monogenea). have identified transcript its coding gene transcriptome adult worms. Its amino acid sequence is similar other stefins except for containing legumain-binding domain, which this cystatins...
Abstract Plectin, a member of the cytolinkers protein family, plays crucial role in cells as stabilizing element against mechanical stress. Its absence results muscular dystrophy, skin blistering, and signs neuropathy. The C‐terminal domain plectin contains several highly homologous repeat domains that also occur other cytolinkers. Secondary structure analysis revealed building block these domains, PLEC repeat, is similar to ankyrin repeat. We present model attempts explain how domain, which...
Based on cDNA cloning and sequencing, human epiplakin has been classified as a member of the plakin protein family cytolinkers. We report here characterization mouse gene locus isolation full-length using BAC vectors. found that is encoded by single remarkably large exon (>20 kb) consists series 0.8–1.5-kb-long DNA repeats, eight which are virtually identical. Consequently, contains 16 repeat domains, three more than reported for predicted based contig characterized Mouse Genome Sequencing...
Abstract A tert ‐butoxide base‐mediated cyclization of fluoro‐ and trifluoromethyl‐substituted Schiff bases 12‐19 produces 4‐ ‐butoxyquinolines 23‐30 which are hydrolyzed to quinolin‐4‐ols 31‐38 .
Antibacterial antibiotic therapy has played an important role in the treatment of bacterial infections for almost a century. The increasing resistance pathogenic bacteria to antibiotics leads attempt use previously neglected antibacterial therapies. Here we provide information on two recombinantly modified antistaphylococcal enzymes derived from lysostaphin (LYSSTAPH-S) and endolysin (LYSDERM-S) kayvirus 812F1 whose target sites reside cell wall. LYSSTAPH-S showed stable antimicrobial effect...
Abstract The interaction of a series 2‐phenylquinoline derivatives with RNA was investigated by means viscometric, pKa, spectroscopic, binding, T m , and kinetic methods. Compounds 1 2 3 have piperazyl substituent at the para meta or ortho position, respectively, while 4 has an unsubstituted phenyl ring. pKa results suggest that three charges, more than two charges pH 6.2. Spectroscopic indicate binds strongly to 2–4 . Kinetic modeling is threading intercalator are classical intercalators....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHighly regioselective bromination reactions of polymethylpyrimidinesLucjan Strekowski, Roman L. Wydra, Lubomir Janda, and Donald B. HardenCite this: J. Org. Chem. 1991, 56, 19, 5610–5614Publication Date (Print):September 1, 1991Publication History Published online1 May 2002Published inissue 1 September 1991https://pubs.acs.org/doi/10.1021/jo00019a028https://doi.org/10.1021/jo00019a028research-articleACS PublicationsRequest reuse permissionsArticle...
β‐Glucosidases such as Zm‐p60.1 ( Zea mays ) and Bgl4:1 Brassica napus have implicated roles in regulating plant development by releasing biologically active cytokinins from O ‐glucosides. A key determinant of substrate specificity is the F193–F200–W373–F461 cluster. However, despite sharing same substrates, amino acids sites differ dramatically. In members Brassicaceae we found a group β‐glucosidases both high similarity to consensus motif A‐K‐K‐L corresponding To study mechanism further,...
Multistep phosphorelay (MSP) cascades mediate responses to a wide spectrum of stimuli, including plant hormonal signaling, but several aspects MSP await elucidation. Here, we provide first insight into the key step MSP-mediated phosphotransfer in eukaryotic system, phosphorylation receiver domain histidine kinase CYTOKININ-INDEPENDENT 1 (CKI1RD) from Arabidopsis thaliana. We observed that crystal structures free, Mg2+-bound, and beryllofluoridated CKI1RD (a stable analogue labile...
Abstract Antimicrobial peptides, including the human cathelicidin LL-37, offer a possible solution to global problem of bacterial resistance antibiotics. LL-37 peptide has potent antimicrobial effects against current multi-drug resistant strains. The itself is also characterized by very diverse range immunomodulatory effects. aim this study was produce antimicrobially active in E. coli high yields using an own expression system pUbEx100 with fusion protein ubiquitin. results showed that...
WD-repeat proteins are found in all eukaryotes and implicated a variety of regulatory functions as result protein–protein interactions. PkwA from Thermomonospora curvata CCM3352 is first potential example protein prokaryotic actinomycete. A mAb (3G2) was generated against the carboxy terminus used to analyse expression T. curvata. detected exponential growth phase following inoculation with spores, but could not be at any stage vegetative mycelium. its WD domain were expressed Escherichia...