A5006644897- 14-3-3 protein interactions
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Insect Resistance and Genetics
- Plant nutrient uptake and metabolism
- Protein Structure and Dynamics
- Insect and Arachnid Ecology and Behavior
- Photosynthetic Processes and Mechanisms
- Silk-based biomaterials and applications
- Enzyme Structure and Function
- Neurobiology and Insect Physiology Research
- Plant Stress Responses and Tolerance
- Protein Kinase Regulation and GTPase Signaling
- Insect Utilization and Effects
- Plant Reproductive Biology
- Mass Spectrometry Techniques and Applications
- Advancements in Transdermal Drug Delivery
- Plant Molecular Biology Research
- ATP Synthase and ATPases Research
- Plant and Biological Electrophysiology Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Acne and Rosacea Treatments and Effects
- Glycosylation and Glycoproteins Research
- Dermatology and Skin Diseases
- Phosphodiesterase function and regulation
Central European Institute of Technology
2011-2025
Central European Institute of Technology – Masaryk University
2011-2025
L'Oréal (France)
2024
Masaryk University
2011-2018
Central European Institute of Technology
2017
Lund University
2006-2007
Microtubule associated protein 2 (MAP2) interacts with the regulatory 14‐3‐3ζ in a cAMP‐dependent kinase (PKA) phosphorylation dependent manner. Using selective phosphorylation, calorimetry, nuclear magnetic resonance, chemical crosslinking, and X‐ray crystallography, we characterized interactions of various binding regions MAP2c. Although PKA increases affinity MAP2c for proline rich region C‐terminal domain, unphosphorylated also binds dimeric via its microtubule domain variable central...
Abstract Chemosensory Proteins (CSPs) represent a family of conserved proteins found in insects that may be involved chemosensory functions. BmorCSP1 is expressed mainly antennae and legs the silkworm moth Bombyx mori was cloned from antennal cDNA. Here we report determination structure CSP1 (BmorCSP1) by NMR. The overall fold globular comprises six α‐helices. These helices span residues 10–14, 17–27, 35–49, 57–72, 75–85, 92–100. internal hydrophobic sides are formed mostly leucine...
Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via phosphotransfer proteins (AHP1-AHP5) nuclear response regulators. contrast ancestral two-component bacteria, protein interactions plant MSP are supposed be rather nonspecific. Here, we show that C-terminal receiver domain HK CKI1 (CKI1(RD) ) responsible for recognition downstream partners, and specifically...
Microtubule-associated protein 2c (MAP2c) is a 49-kDa intrinsically disordered regulating the dynamics of microtubules in developing neurons. MAP2c differs from its sequence homologue Tau pattern and kinetics phosphorylation by cAMP-dependent kinase (PKA). Moreover, mechanisms through which interacts with binding partners conformational changes associated these interactions remain unclear. Here, we used NMR relaxation paramagnetic enhancement techniques to determine long-range within MAP2c....
Multistep phosphorelay (MSP) cascades mediate responses to a wide spectrum of stimuli, including plant hormonal signaling, but several aspects MSP await elucidation. Here, we provide first insight into the key step MSP-mediated phosphotransfer in eukaryotic system, phosphorylation receiver domain histidine kinase CYTOKININ-INDEPENDENT 1 (CKI1RD) from Arabidopsis thaliana. We observed that crystal structures free, Mg2+-bound, and beryllofluoridated CKI1RD (a stable analogue labile...
Microtubule-associated protein 2 (MAP2) is an important neuronal target of extracellular signal-regulated kinase (ERK2) involved in Raf signaling pathways, but mechanistic details MAP2 phosphorylation are unclear. Here, we used NMR spectroscopy to quantitatively describe the kinetics individual serines and threonines embryonic variant MAP2c. We carried out real-time monitoring discover major sites that were not identified previous studies relying on specific antibodies. Our comparison with...
ABSTRACT Sequence dependence of 13 C and 15 N chemical shifts in the receiver domain CKI1 protein from Arabidopsis thaliana , RD its complexed form, •Mg 2+ was studied by means MD/DFT calculations. MD simulations a 20–ns production run length were performed. Nine explicitly hydrated structures increasing complexity explored, up to 40‐amino‐acid structure. The size model necessary depended on type nucleus, amino acid sequence neighbors, other spatially close acids, orientation NH groups their...
Water molecules can interact with aromatic moieties using either their O-H bonds or lone-pairs of electrons. In proteins, water-π interactions have been reported to occur tryptophan and histidine residues, dynamic exchange between O-Hπ hydrogen bonding lone-pairπ was suggested take place, based on ab initio calculations. Here we used classical QM/MM molecular dynamics simulations, complemented an NMR study, examine a specific water-indole interaction observed in the engrailed homeodomain its...