A5080774116- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Viral gastroenteritis research and epidemiology
- Autoimmune Bullous Skin Diseases
- Toxin Mechanisms and Immunotoxins
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Virology and Viral Diseases
- Plant and Fungal Interactions Research
- Bacterial Identification and Susceptibility Testing
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
- Animal Virus Infections Studies
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- Nail Diseases and Treatments
- Biofuel production and bioconversion
- Molecular Biology Techniques and Applications
Masaryk University
2015-2025
Abstract To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding adaptation processes primed by metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize non-native sugar D-xylose, but its assimilation into bacterial biochemical network via exogenous xylose...
Abstract Lytic bacteriophages are valuable therapeutic agents against bacterial infections. There is continual effort to obtain new phages increase the effectivity of phage preparations emerging phage-resistant strains. Here we described genomic diversity spontaneous host-range mutants kayvirus 812. Five mutant were isolated as rare plaques on Staphylococcus aureus The host range 812-derived was 42% higher than wild type, determined a set 186 methicillin-resistant S. strains representing...
Staphylococcus epidermidis is a leading opportunistic pathogen causing nosocomial infections that notable for its ability to form biofilm and high rates of antibiotic resistance. It serves as reservoir multiple antimicrobial resistance genes spread among the staphylococcal population by horizontal gene transfer such transduction. While phage-mediated transduction well studied in aureus, S. transducing phages have not been described detail yet. Here, we report characteristics four phages, 27,...
Abstract Antibiotic-resistant strains of Staphylococcus aureus pose a significant threat in healthcare, demanding urgent therapeutic solutions. Combining bacteriophages with conventional antibiotics, an innovative approach termed phage-antibiotic synergy, presents promising treatment avenue. However, to enable new strategies, there is pressing need for methods assess their efficacy reliably and rapidly. Here, we introduce novel real-time monitoring pathogen lysis dynamics employing the...
ABSTRACT Macrococci are usually found as commensals on the skin and mucosa of animals have been isolated from mammal-derived fermented foods; however, they can also act opportunistic pathogens. Here, we used whole-genome sequencing, comparative genomics, extensive biotyping, MALDI-TOF mass spectrometry, chemotaxonomy to characterize Macrococcus sp. strains livestock human-related specimens. Based results polyphasic taxonomy, propose species psychrotolerans nov. (type strain NRL/St 95/376 T =...
ABSTRACT Microviruses are single-stranded DNA viruses infecting bacteria, characterized by T = 1 shells made of single jelly-roll capsid proteins. To understand how microviruses infect their host cells, we have isolated and studied an unusually large microvirus, Ebor. Ebor belongs to the proposed “Tainavirinae” subfamily Microviridae infects model Alphaproteobacterium Rhodobacter capsulatus . Using cryogenic electron microscopy, show that enlarged is result extended C-terminus major protein....
Prophages play an important role in virulence, pathogenesis, and host preference, as well horizontal gene transfer staphylococci. In contrast, broad-host-range lytic staphylococcal kayviruses lyse most S. aureus strains, scientists worldwide have come to believe that the use of such phages will be successful for treating preventing bacterial diseases.
Staphylococcus aureus is one of the most frequent pathogens infecting respiratory tract patients with cystic fibrosis (CF). This study was first to examine S. isolates from CF in Czech Republic. Among 100 92 107 observed patients, we found a high prevalence resistance macrolide–lincosamide–streptogramin B (MLSB) antibiotics (56%). More than half resistant strains (29 56) carried mutation MLSB target site. The emergence and mutations conferring associated azithromycin treatment (p=0.000000184...
Staphylococci from the Staphylococcus intermedius-Staphylococcus hyicus species group include numerous animal pathogens and are an important reservoir of virulence antimicrobial resistance determinants. Due to their pathogenic potential, they possible causative agents zoonoses in humans; therefore, it is address properties these strains. Here we used a polyphasic taxonomic approach characterize coagulase-negative staphylococcal strain NRL/St 03/464T, isolated nostrils healthy laboratory rat...
ABSTRACT Novel species of coagulase-negative staphylococci, which could serve as reservoirs virulence and antimicrobial resistance factors for opportunistic pathogens from the genus Staphylococcus , are recognized in human animal specimens due to advances diagnostic techniques. Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, chemotaxonomy characterize five strains haemolyticus phylogenetic clade obtained ear swabs, wounds, bile. Based on results...
Phages with contractile tails employ elaborate strategies to penetrate bacterial cell walls and deliver their genomes into the host cytoplasm. Most tailed phages that have been structurally characterized date infect Gram-negative bacteria, but those targeting Gram-positive many of which are important human pathogens, less well understood. Here, we show baseplate phage phi812, infects Staphylococcus aureus , is formed a core, wedge modules, arms carrying receptor-binding proteins type 1 2...
Single-stranded DNA bacteriophages of the