A5054762808- NF-κB Signaling Pathways
- Advanced Fluorescence Microscopy Techniques
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Platelet Disorders and Treatments
- Photosynthetic Processes and Mechanisms
- interferon and immune responses
- Cellular transport and secretion
- Cell death mechanisms and regulation
- Cell Image Analysis Techniques
- Atherosclerosis and Cardiovascular Diseases
- Peroxisome Proliferator-Activated Receptors
- Extracellular vesicles in disease
- Inflammatory Biomarkers in Disease Prognosis
- bioluminescence and chemiluminescence research
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Prostate Cancer Treatment and Research
- Venous Thromboembolism Diagnosis and Management
- Advanced Biosensing Techniques and Applications
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- Endoplasmic Reticulum Stress and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Medical University of Vienna
2016-2025
Universität Ulm
2016-2023
University of Vienna
1994-2021
Universität Hamburg
2021
The University of Texas MD Anderson Cancer Center
2020
Thrombosis Research Institute
2020
Czech Academy of Sciences, Institute of Physiology
2018
University of Regensburg
2012
Ludwig Boltzmann Institute for Cancer Research
2003-2008
MRC Laboratory of Molecular Biology
2008
By differential screening of tumor necrosis factor α (TNF-α) and lipopolysaccharide (LPS)- activated endothelial cells (ECs), we have identified a cDNA clone that turned out to be member the inhibitor apoptosis (iap) gene family. iap genes function protect from undergoing apoptotic death in response variety stimuli. These genes, hiap1, hiap2, xiap were found strongly upregulated upon treatment ECs with inflammatory cytokines TNF-α, interleukin 1β, LPS, reagents lead activation nuclear...
Immune cells are somewhat unique in that activation responses can alter quantitative phenotypes upwards of 100,000-fold. To date little is known about the metabolic adaptations necessary to mount such dramatic phenotypic shifts. Screening for novel regulators macrophage activation, we found nonprotein kinases glucose metabolism among most enriched classes candidate immune modulators. We find one these, carbohydrate kinase-like protein CARKL, rapidly downregulated vitro and vivo upon LPS...
We aimed to investigate the dynamics of NF-κB signaling pathway in living cells using GFP variants p65-NF-κB, IκBα, tumor necrosis factor-receptor associated factor 2 (TRAF2), inducing kinase (NIK) and IκB kinases (IKK1 IKK2). Detailed kinetic analysis constitutive nucleocytoplasmic shuttling processes revealed that IκBα enters nucleus faster than p65. Examination molecules upstream a predominant cytoplasmic localization at steady state. However, after addition leptomycin B, NIK rapidly...
We have recently shown that stimulation of TNF-R2 selectively enhances apoptosis induction by the death receptor TNF-R1. Here, we demonstrate CD30 or CD40 also leads to selective enhancement TNF-R1-induced cell death. Enhancement was correlated with depletion endogenous TRAF2 within 1 6 hours. Selective prestimulation for several hours inhibited TNF-R2-induced activation anti-apoptotic NF-κB pathway up 90% and dramatically enhanced this receptor. When both TNF-receptors were stimulated...
Exposure of endothelial and many other cell types to tumor necrosis factor α generates both apoptotic anti-apoptotic signals. The pathway leads activation the transcription NF-κB that regulates expression genes such as A20 or members IAP gene family protect cells from α-mediated apoptosis. In turn, some have been shown modulate activity. Here we demonstrate XIAP, a NF-κB-dependent member family, is strong stimulator NF-κB. Expression XIAP increased nuclear translocation p65 subunit via novel...
Recent biochemical studies indicate that the serotonin transporter can form oligomers. We investigated whether human (hSERT) be visualized as an oligomer in plasma membrane of intact cells. For this purpose, we generated fusion proteins hSERT and spectral variants green fluorescent protein (cyan yellow proteins, CFP YFP, respectively). When expressed embryonic kidney 293 cells, resulting (CFP-hSERT YFP-hSERT) were efficiently inserted into functionally indistinguishable from wild-type hSERT....
Abstract Purpose: Constitutive activation of inhibitor κB kinase (IKK) confers melanoma resistance to apoptosis and chemotherapy. Whether IKK is able serve as a therapeutic target in unknown. We explored the possibility exploiting by using BMS-345541, novel compound with highly selective IKKβ inhibitory activity, trigger cell apoptosis. Experimental Design: Three human lines (SK-MEL-5, Hs 294T, A375), all which have high constitutive activities, served vitro vivo models for treatment...
The question whether two proteins interact with each other or a protein localizes to certain region of the cell is often addressed fluorescence microscopy and analysis potential colocalization markers. Since mere visual estimation does not allow quantification degree colocalization, different statistical methods pixel-intensity correlation are commonly used score it. We observed that these coefficients prone false positive results tend show high values even for molecules reside in...
Na+/Cl–-dependent neurotransmitter transporters form constitutive oligomers. The topological arrangement is not known, but a leucine heptad repeat in transmembrane domain (TM) 2 and glycophorin-like motif TM6 have been proposed to stabilize the oligomer. To determine topology, we generated versions of human serotonin transporter (hSERT) that carried cyan or yellow fluorescent proteins at their amino and/or carboxyl terminus. Appropriate pairs were coexpressed measure fluorescence resonance...
Re-uptake of gamma-aminobutyric acid (GABA) into presynaptic specializations is mediated by the GABA transporter 1 (GAT1), a member SLC6 gene family. Here, we show that motif in COOH terminus GAT1 ((566)RL(567)), which conserved family members, binding site for COPII coat component Sec24D. We also identified residues Sec24D ((733)DD(734)) are required to support interaction with and two additional i.e. transporters serotonin dopamine. used three strategies prevent recruitment GAT1:...
Abstract Tissue remodeling or regeneration is believed to initiate from multipotent stem and progenitor cells. We report here the establishment of two spontaneously immortalized adult non-tumorigenic human prostate epithelial cell lines, NHPrE1 BHPrE1. (CD133high/CD44high/OCT4high/PTENhigh) was characterized as a putative cell, BHPrE1 (p63high/p53high/p21(WAF1)high/RBhigh) intermediate cell. Genomic analysis demonstrated an abnormal karyotype with genomic rearrangements including PTEN...
Platelets store a plethora of different molecules within their granules, modulating numerous pathways, not only in coagulation, but also angiogenesis, wound healing, and inflammatory diseases. These get rapidly released upon activation therefore represent an easily accessible indirect marker for platelet activation. Accurate analysis platelet-derived the plasma requires appropriate anticoagulation to avoid vitro subsequent degranulation platelets, potentially causing artificially high levels...
Binary enterotoxins Clostridium (C.) botulinum C2 toxin, C. perfringens iota toxin and difficile CDT are composed of a transport (B) separate non-linked enzyme (A) component. Their B-components mediate endocytic uptake into mammalian cells subsequently the A-components from acidic endosomes cytosol, where latter ADP-ribosylate G-actin resulting in cell rounding death causing clinical symptoms. Protein folding enzymes, including Hsp90 peptidyl-prolyl cis/trans isomerases facilitate across...
Microscopy of stained blood smears is still a ubiquitous technique in pathology. It often used addition to automated electronic counters or flow cytometers evaluate leukocytes and their morphologies rather simple manner has low requirements for resources equipment. However, despite the constant advances microscopy, computer science, pathology, it usually follows traditional approach manual assessment by humans. We aimed extend this using AI-based cell recognition methods while maintaining...