A5066576863- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- ATP Synthase and ATPases Research
- Spectroscopy and Quantum Chemical Studies
- Plant Stress Responses and Tolerance
- Light effects on plants
- Advanced Electron Microscopy Techniques and Applications
- Plant responses to elevated CO2
- Metalloenzymes and iron-sulfur proteins
- Antioxidant Activity and Oxidative Stress
- RNA modifications and cancer
- Potato Plant Research
- Plant Micronutrient Interactions and Effects
- Microbial Fuel Cells and Bioremediation
- Autophagy in Disease and Therapy
- Plant Water Relations and Carbon Dynamics
- Parkinson's Disease Mechanisms and Treatments
- Enzyme Structure and Function
- Diatoms and Algae Research
- Plant Parasitism and Resistance
- Alzheimer's disease research and treatments
- Spectroscopy and Chemometric Analyses
- Marine and coastal ecosystems
Palacký University Olomouc
2013-2025
University of Groningen
2003-2013
Electron (Ukraine)
2008-2010
Molecular Research Institute
2009
University of Amsterdam
2004
Chloroplasts are central to the provision of energy for green plants. Their photosynthetic membrane consists two major complexes converting sunlight: photosystem I (PSI) and II (PSII). The flow toward both photosystems is regulated by light-harvesting complex (LHCII), which after phosphorylation can move from PSII PSI in so-called state 1 2 transition back dephosphorylation. To investigate changes during transitions, we studied structures frequencies all Arabidopsis thaliana chloroplasts at...
Mitochondrial respiratory chain complexes are arranged in supercomplexes within the inner membrane. Interaction of cytochrome c reductase (complex III) and oxidase IV) was investigated Saccharomyces cerevisiae. Projection maps at 15 A resolution III(2) + IV(1) IV(2) were obtained by electron microscopy. Based on a comparison our with atomic x-ray structures for III IV we present pseudo-atomic model their precise interaction. Two complex monomers specifically attached to dimeric convex sides....
The role of the light-harvesting complex Lhcb4 (CP29) in photosynthesis was investigated Arabidopsis thaliana by characterizing knockout lines for each three isoforms (Lhcb4.1/4.2/4.3). Plants lacking all (koLhcb4) showed a compensatory increase Lhcb1 and slightly reduced photosystem II/I ratio with respect to wild type. absence did not result alteration electron transport rates. However, kinetic state transition faster mutant, nonphotochemical quenching activity lower koLhcb4 plants either...
Summary Photo‐reduction of O 2 to water mediated by flavodiiron proteins ( FDP s) represents a safety valve for the photosynthetic electron transport chain in fluctuating light. So far, ‐mediated photo‐reduction has been evidenced only cyanobacteria and moss Physcomitrella ; however, recent phylogenetic analysis transcriptomes organisms also revealed presence genes several nonflowering plant groups. What remains be clarified is whether ‐dependent actually operational these organisms. We have...
A significant part of global primary productivity is provided by cyanobacteria, which are abundant in most marine and freshwater habitats. In many oceanographic regions, however, the concentration iron can be so low that it limits growth. Cyanobacteria respond to this condition expressing a number stress inducible genes, isiA gene encodes chlorophyll-binding protein known as IsiA or CP43'. It was recently shown 18 proteins encircle trimeric photosystem I (PSI) under iron-deficient growth...
The PsbS protein is a critical component in the regulation of non‐photochemical quenching (NPQ) higher plant photosynthesis. Electron microscopy and image analysis grana membrane fragments from wild type mutant Arabidopsis plants showed that semi‐crystalline domains photosystem II supercomplexes were identical presence absence PsbS. However, frequency containing crystalline arrays was increased Conversely, there complete such membranes elevated amounts this protein. It proposed controls...
In this work we have purified the Photosystem I (PSI) complex of Chlamydomonas reinhardtii to homogeneity. Biochemical, proteomic, spectroscopic, and structural analyses reveal main properties PSI-LHCI supercomplex. The data show that largest is composed one core nine Lhca antennas it contains all gene products. A projection map at 15 Å resolution obtained by electron microscopy reveals Lhcas are organized on side in a double half-ring arrangement, contrast with previous suggestions. series...
Summary Cyclic electron transport ( CET ) around photosystem I PSI plays an important role in balancing the ATP / NADPH ratio and photoprotection of plants. The NAD(P)H dehydrogenase complex NDH has a key function one pathways. Current knowledge indicates that, order to fulfill its , needs be associated with ; however, until now there been no direct structural information about such supercomplex. Here we present data obtained for plant – Electron microscopy analysis revealed that this...
Plants are exposed to continuous changes in light quality and quantity that challenge the performance of photosynthetic apparatus have evolved a series mechanisms face this challenge. In work, we studied state transitions, process redistributes excitation pressure between photosystems I II (PSI/PSII) by reversible association LHCII, major antenna complex higher plants, with either one them upon phosphorylation/dephosphorylation. By combining biochemical analysis electron microscopy, effect...
Summary Plant photosystem II (PSII) is organized into large supercomplexes with variable levels of membrane‐bound light‐harvesting proteins (LHCIIs). The largest stable form the PSII supercomplex involves four LHCII trimers, which are specifically connected to core dimer via monomeric antenna proteins. can further interact in thylakoid membrane, forming megacomplexes. So far, only megacomplexes consisting two associated parallel have been observed. Here we show that forms be much more...
Summary Photosynthesis in plants and algae relies on the coordinated function of photosystems ( PS ) I II . Their efficiency is augmented by finely‐tuned light‐harvesting proteins (Lhcs) connected to them. The most recent Lhcs (in evolutionary terms), Lhcb6 Lhcb3, evolved during transition from water land have so far been considered be an essential characteristic plants. We used single particle electron microscopy sequence analysis study architecture composition PSII supercomplex Norway...
Photosynthetic light-harvesting antennae are pigment-binding proteins that perform one of the most fundamental tasks on Earth, capturing light and transferring energy enables life in our biosphere. Adaptation to different environments led evolution an astonishing diversity systems. At same time, several strategies have been developed optimize input into photosynthetic membranes response fluctuating conditions. The basic feature these prompt responses is dynamic nature antenna complexes,...
Light-harvesting protein LHCB5 is one of the three minor antenna proteins (LHCB4-6) that connect core (C) photosystem II (PSII) with strongly (S) and moderately (M) bound peripheral trimeric antennae (LHCIIs), forming a dimeric PSII supercomplex known as C2S2M2. Plants lacking LHCB4 LHCB6 do not form C2S2M2, indicating these are crucial for C2S2M2 assembly. However, studies on antisense asLhcb5 plants suggest this may apply to LHCB5. Using mild clear-native PAGE (CN-PAGE) electron microscopy...
The monomeric photosystem I-light-harvesting antenna complex I (PSI-LHCI) supercomplex from the extremophilic red alga Cyanidioschyzon merolae represents an intermediate evolutionary link between cyanobacterial PSI reaction center and its green algal/higher plant counterpart. We show that C. PSI-LHCI is characterized by robustness in various extreme conditions. By a combination of biochemical, spectroscopic, mass spectrometry, electron microscopy/single particle analyses, we dissected three...
Abstract Diatoms are a large group of marine algae that responsible for about one-quarter global carbon fixation. Light-harvesting complexes diatoms formed by the fucoxanthin chlorophyll a/c proteins and their overall organization around core photosystems (PSs) I II is unique in plant kingdom. Using cryo-electron tomography, we have elucidated structural PSII PSI supercomplexes spatial segregation thylakoid membrane model diatom species Thalassiosira pseudonana. 3D sub-volume averaging...