A5015046170- Photosynthetic Processes and Mechanisms
- Extracellular vesicles in disease
- Mitochondrial Function and Pathology
- Advanced Polymer Synthesis and Characterization
- Boron Compounds in Chemistry
- Surfactants and Colloidal Systems
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Genomics and Phylogenetic Studies
- Nanoparticle-Based Drug Delivery
- Supramolecular Chemistry and Complexes
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- RNA Research and Splicing
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Light effects on plants
- Boron and Carbon Nanomaterials Research
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Characterization and Applications of Magnetic Nanoparticles
- ATP Synthase and ATPases Research
- Chemical Synthesis and Characterization
Charles University
2015-2024
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2023-2024
General University Hospital in Prague
2017-2023
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2021
University of Groningen
2006-2010
Molecular Research Institute
2009
Electron (Ukraine)
2007-2009
The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana depleted one these, the CP24 complex, have been analyzed. CP24-deficient showed decrease light-limited photosynthetic rate and growth, but pigment protein content thylakoid membranes were otherwise almost unchanged. However, there was major change macroorganization PSII within these membranes; electron microscopy image...
Abstract The main trimeric light-harvesting complex of higher plants (LHCII) consists three different Lhcb proteins (Lhcb1-3). We show that Arabidopsis thaliana T-DNA knockout lacking Lhcb3 (koLhcb3) compensate for the lack by producing increased amounts Lhcb1 and Lhcb2. As in wild-type plants, LHCII-photosystem II (PSII) supercomplexes were present (koLhcb3), preservation LHCII trimers (M trimers) indicates M has been replaced and/or However, rotational position trimer was altered,...
The PsbS protein is a critical component in the regulation of non‐photochemical quenching (NPQ) higher plant photosynthesis. Electron microscopy and image analysis grana membrane fragments from wild type mutant Arabidopsis plants showed that semi‐crystalline domains photosystem II supercomplexes were identical presence absence PsbS. However, frequency containing crystalline arrays was increased Conversely, there complete such membranes elevated amounts this protein. It proposed controls...
PsbW, a 6.1-kDa low-molecular-weight protein, is exclusive to photosynthetic eukaryotes, and associates with the photosystem II (PSII) protein complex. In vivo in vitro comparison of Arabidopsis thaliana wild-type plants T-DNA insertion knock-out mutants completely lacking PsbW or antisense inhibition exhibiting decreased levels demonstrated that loss destabilizes supramolecular organization PSII. No PSII-LHCII supercomplexes could be detected isolated absence protein. These changes...
The classical “hard corona–soft corona” paradigm is not valid for HPMA-based nanoparticles.
Abstract African Trypanosomes have developed elaborate mechanisms to escape the adaptive immune response, but little is known about complement evasion particularly at early stage of infection. Here we show that ISG65 human-infective parasite Trypanosoma brucei gambiense a receptor for human factor C3 and its activation fragments it takes over role in selective inhibition alternative pathway C5 convertase thus abrogation terminal pathway. No deposition C4b, as part classical lectin...
Stimuli-responsive copolymers are of great interest for targeted drug delivery. This study reports on a controllable post-polymerization quaternization with 2-bromomethyl-4-fluorophenylboronic acid the poly(4-vinyl pyridine) (P4VP) block common poly(styrene)-b-poly(4-vinyl pyridine)-b-poly(ethylene oxide) (SVE) triblock terpolymer in order to achieve selective responsivity various diols. For this purpose, reproducible method was established P4VP at defined ratio, confirming reaction yield by...
Mitochondria originated from proteobacterial endosymbionts, and their transition to organelles was tightly linked establishment of the protein import pathways. The initial most proteins is mediated by translocase outer membrane (TOM). Although TOM common all forms mitochondria, an unexpected diversity subunits between eukaryotic lineages has been predicted. However, experimental knowledge limited a few organisms, so far, it remains unsettled whether triplet-pore or twin-pore structure...
Abstract Lon is an essential, multitasking AAA + protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels the human mitochondrial ( h Lon) are linked to serious diseases including myopathies, paraplegia, and cancer. Here, we present first 3D structure full-length using cryo-electron microscopy. has a unique three-dimensional structure, which proteolytic ATP-binding domains (AP-domain) form hexameric chamber, while N-terminal domain...
The radiation-resistant bacterium Deinococcus radiodurans is known as the world's toughest bacterium. S-layer of D. radiodurans, consisting several proteins on surface cellular envelope and intimately associated with outer membrane, has therefore been useful a model for structural functional studies. Its main proteinaceous unit, deinoxanthin-binding complex (SDBC), hetero-oligomeric assembly to contribute resistance against environmental stress have porin features; however, its precise...
Abstract Lipid nanoparticles (LNPs) are the most advanced nonviral modality for nucleic acid (NA) delivery, and have recently gained enormous attention in fields of RNA therapeutics vaccine development. Here, ionizable adamantane‐based lipidoids named XMaNs, which circumvent usual need laborious optimization LNP components highly diverse types NAs, described. The non‐toxic XMaN6 lipidoid is versatile entrapment delivery siRNA, mRNA, plasmid DNA, a cyclic dinucleotide. XMaN6‐based LNPs...
Bacterial surface layers are paracrystalline assemblies of proteins that provide the first line defense against environmental shocks. Here, we report 3D structure, in situ localization, and orientation S-layer deinoxanthin-binding complex (SDBC), a hetero-oligomeric assembly Deinococcus radiodurans represents main unit. The SDBC is resolved at 11-Å resolution by single-particle analysis, while its localization determined cryo-electron crystallography on intact cell-wall fragments leading to...
The integral membrane light‐harvesting (LH) proteins from purple photosynthetic bacteria form circular oligomers of an elementary unit that is composed two very hydrophobic polypeptides, termed α and β. These apoprotein dimers are known to associate into closed arrays 8, 9 16 α/β‐mers. We report the existence peripheral LH purified Allochromatium vinosum with intermediate ring sizes postulate one a 13 α/β‐mer. This shows able rings continuously increasing diameter 68 115 Å. presence these...
The formation kinetics and structure of supported lipid bilayers embedded with quantum-dots (QDs) depend on the QD size.
In the extremophile bacterium Deinococcus radiodurans, outermost surface layer is tightly connected with rest of cell wall. This integrated organization provides a compact structure that shields against environmental stresses. The fundamental unit this (S-layer) S-layer deinoxanthin-binding complex (SDBC), which binds carotenoid deinoxanthin and both, thermostability UV radiation resistance. However, structural SDBC awaits elucidation. Here, we report isolation gentle procedure consisting...
There are huge demands on multifunctional nanocarriers to be used in nanomedicine. Herein, we present a simple and efficient method for the preparation of magnetically responsive polymeric-based optimized biomedical applications. The hybrid delivery system is composed drug-loaded polymer nanoparticles (poly(caprolactone), PCL) coated with multilayer shell polyglutamic acid (PGA) superparamagnetic iron oxide (SPIONs), which known as bio-acceptable components. PCL model anticancer drug...
Extracellular vesicles (EVs) are membranous structures in biofluids with enormous diagnostic/prognostic potential for application liquid biopsies. Any such downstream requires a detailed characterization of EV concentration, size and morphology. This study aimed to observe the native morphology EVs human cerebrospinal fluid after traumatic brain injury. Therefore, they were separated by gravity-driven size-exclusion chromatography (SEC) investigated atomic force microscopy (AFM) cryogenic...
ABSTRACT Adenovirus (AdV) infection of the respiratory epithelium is common but poorly understood. Human AdV species C types, such as HAdV-C5, utilize Coxsackie-adenovirus receptor (CAR) for attachment and subsequently integrins entry. CAR are however located deep within tight junctions in mucosa where they would not be easily accessible. Recently, a model CAR-independent entry was proposed. In this model, human lactoferrin (hLF), an innate immune protein, aids viral uptake into epithelial...
Amphiphilic triblock terpolymers have attractive applications in the preparation of nanoparticles with controlled morphology.
This work focuses on the synthesis and aqueous solution properties of novel amphiphilic PCL-b-PHPMA diblock copolymers possessing 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) stable radicals covalently conjugated to hydrophobic poly(ε-caprolactone) (PCL) block. A new synthetic approach (a four-step pathway) combining ring-opening polymerization (ROP), carbodiimide chemistry (DCC method), a reversible addition–fragmentation chain transfer (RAFT) technique, finally chemical oxidation was...
Abstract Boron‐rich particles with the boron fraction ca.10–20 wt % of controllable shape and size that can be easily prepared via simple ion co‐assembly are promising material for tumor treatment by neutron capture therapy. Electroneutral, dynamic core‐shell polymeric nanoparticles were cationic PEO‐ block ‐PGEA diblock copolymer sodium closo ‐dodecaborate, Na 2 [B 12 H ]. This is first example polymer based on ] 2− nano‐ion pairing. The high loading proven calorimetry at physiological salt...