A5033472075- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Silicon and Solar Cell Technologies
- Photoreceptor and optogenetics research
- Semiconductor materials and interfaces
- Mass Spectrometry Techniques and Applications
- Algal biology and biofuel production
- ATP Synthase and ATPases Research
- Solidification and crystal growth phenomena
- Redox biology and oxidative stress
- Antioxidant Activity and Oxidative Stress
- Plant and animal studies
- Silicon Nanostructures and Photoluminescence
- Light effects on plants
- Insect and Pesticide Research
- Analytical chemistry methods development
- Advanced Proteomics Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Insect and Arachnid Ecology and Behavior
- Analytical Chemistry and Chromatography
Umeå University
2012-2024
Umeå Plant Science Centre
2008-2024
University of Cologne
2024
LVR-Klinik Köln
2024
University Hospital Cologne
2024
Karolinska Institutet
2002-2022
Lodz University of Technology
2016
University of British Columbia
2013
Technical University of Munich
2001-2012
Hamburg University of Technology
1993-2011
The thylakoid membrane of the chloroplast is center oxygenic photosynthesis. To better understand function luminal compartment within network, we have carried out a systematic characterization proteins from model organism Arabidopsis thaliana. Our data show that lumen has its own specific proteome, which 36 were identified. Besides large group peptidyl-prolyl cis-trans isomerases and proteases, family novel PsbP domain was found. An analysis signal peptides showed 19 precursors marked by...
Photosystem II starts the photosynthetic electron transport chain that converts solar energy into chemical and thus sustains life on Earth. It catalyzes two reactions: water oxidation to molecular oxygen plastoquinone reduction. Coupling of proton transfer is crucial for efficiency; however, basis these processes remains speculative owing uncertain binding sites lack experimentally determined hydrogen positions. We collected high-resolution cryo-electron microscopy data fully hydrated...
In the Brenta area of northern Italy, a brown bear Ursus arctos population is rapidly going extinct. Restocking planned. order to study genetics this highly vulnerable with minimum stress animals we have developed PCR-based method that allows mitochondrial and nuclear gene sequences from droppings collected in field. This generally applicable wild. Using excremental as well hair samples, show monomorphic for one lineage female male bears exist area. addition, 70 samples other parts Europe...
Summary Cold acclimation and over‐wintering by herbaceous plants are energetically expensive dependent on functional plastid metabolism. To understand how the stroma lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) identify proteins that changed in abundance Arabidopsis chloroplasts during cold shock (1 day), short‐ (10 days) long‐term (40 5°C. We show day) results minimal change proteomes, while short‐term major changes stromal...
Native phosphorylated mouse small heat shock protein hsp25 from Ehrlich ascites tumor cells was isolated and the in vivo phosphorylation sites of were determined. Furthermore, native by endogenous kinase(s) a cell-free system as well recombinant vitro kinase C catalytic subunit cAMP-dependent kinase. The two major determined Ser-15 Ser-86. There are no differences C, unknown intracellular kinase(s). serine residues identified exist all known mammalian stress proteins located conserved...
Abstract Background Plants exhibit phenotypic plasticity and respond to differences in environmental conditions by acclimation. We have systematically compared leaves of Arabidopsis thaliana plants grown the field under controlled low, normal high light laboratory determine their most prominent differences. Results Compared conditions, "indoor plants" had larger leaves, modified leaf shapes longer petioles. Their pigment composition also significantly differed; indoor reduced levels...
The chloroplast compartment enclosed by the thylakoid membrane, "lumen," is poorly characterized. major aims of this work were to design a procedure for isolation lumen which could be generally used characterize lumenal proteins. preparation was stepwise in membranes isolated from intact chloroplasts. Loosely associated surface proteins removed, and following Yeda press fragmentation content recovered supernatant centrifugation. purity yield determined using appropriate marker specific...
In this report, we describe a newly developed method for isolating outer membranes from Synechocystis sp. PCC 6803 cells. The purity of the membrane fraction was verified by immunoblot analysis using antibodies against membrane-specific marker proteins. We investigated protein composition two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry followed database identification. Forty-nine proteins were identified corresponding to 29...
Abstract The light‐dependent regulation of stromal enzymes by thioredoxin (Trx)‐catalysed disulphide/dithiol exchange is known as a classical mechanism for control chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but all compartments, from envelope to thylakoid lumen. Trx‐mediated redox appears be common feature important pathways, such Calvin cycle, starch synthesis and tetrapyrrole biosynthesis. However, extent thiol‐dependent lumen...
Summary Siberian spruce ( P icea obovata ) is one of several boreal conifer species that can survive at extremely low temperatures ELT s). When fully acclimated, its tissues immersion in liquid nitrogen. Relatively little known about the biochemical and biophysical strategies survival. We profiled needle metabolites using gas chromatography coupled with mass spectrometry GC ‐ MS to explore metabolic changes occur during cold acclimation caused by natural temperature fluctuations. In total,...
Chloroplasts develop from undifferentiated proplastids present in meristematic tissue. Thus, chloroplast biogenesis is closely connected to leaf development, which restricts our ability study the process of per se. As a consequence, we know relatively little about regulatory mechanisms behind establishment photosynthetic reactions and how activities two genomes involved are coordinated during development. We developed single cell-based experimental system Arabidopsis (Arabidopsis thaliana)...
Conjugative DNA transfer of the self-transmissible broad-host-range plasmid RP4 is initiated by strand- and site-specific cleavage at nick site (nic) origin (oriT). Cleavage results in covalent attachment plasmid-encoded relaxase (TraI) to 5'-terminal 2'-deoxycytidine residue nic. We demonstrate that Tyr22 center catalytic TraI, mediating via formation a phosphodiester between 5' phosphoryl aromatic hydroxyl group. The specificity seen with form I oriT was verified short...
We describe the isolation and characterization of cDNAs encoding precursor polypeptide 6.1-kDa associated with reaction center core photosystem II complex from spinach. PsbW, gene this polypeptide, is present in a single copy per haploid genome. The mature 54 amino acid residues characterized by hydrophobic transmembrane segment, and, although an intrinsic membrane protein, it carries bipartite transit peptide 83 which directs N terminus protein into chloroplast lumen. Thylakoid integration...
The TraI protein of plasmid RP4 (IncP alpha) catalyzes a site- and strand-specific cleaving-joining reaction on form I or single-stranded DNA. Thus, is one the key components involved in initiation termination horizontal DNA transfer by bacterial conjugation. Amino acid sequence comparison revealed three motifs conserved relaxases from different origins. Site-directed mutagenesis traI structural gene application purified mutant proteins for vitro assays served to evaluate functional...
Some techniques are surveyed of how to monochromatize synchrotron X-radiation and eliminate harmonics in the most appropriate way. The theoretical estimates compared with spectral intensity distribution measurements at DESY Hamburg found be good agreement.