Rana Hussein
A5082874674- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Enzyme Structure and Function
- Photoreceptor and optogenetics research
- Advanced Electron Microscopy Techniques and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Advanced Thermodynamics and Statistical Mechanics
- Mitochondrial Function and Pathology
- Metalloenzymes and iron-sulfur proteins
- DNA and Nucleic Acid Chemistry
- Plant Stress Responses and Tolerance
- Molecular spectroscopy and chirality
- Synthesis of β-Lactam Compounds
- Pharmacological Effects of Natural Compounds
- Tea Polyphenols and Effects
- Hemoglobin structure and function
- Microbial Fuel Cells and Bioremediation
- Healthcare Education and Workforce Issues
- Electron Spin Resonance Studies
- Synthesis and Biological Evaluation
- Diverse Approaches in Healthcare and Education Studies
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Bioactive Compounds and Antitumor Agents
- Stellar, planetary, and galactic studies
Humboldt State University
2025
Humboldt-Universität zu Berlin
2016-2024
University of Manchester
2024
Health Innovation Manchester
2024
University of Sadat City
2023
University of Diyala
2023
University of Sharjah
2020
Universität Hamburg
2014
In oxygenic photosynthesis, light-driven oxidation of water to molecular oxygen is carried out by the oxygen-evolving complex (OEC) in photosystem II (PS II). Recently, we reported room-temperature structures PS four (semi)stable S-states, S
Abstract Light-driven oxidation of water to molecular oxygen is catalyzed by the oxygen-evolving complex (OEC) in Photosystem II (PS II). This multi-electron, multi-proton catalysis requires transport two molecules and four protons from OEC. A high-resolution 1.89 Å structure obtained averaging all S states refining data various time points during 2 3 transition has provided better visualization potential pathways for substrate insertion proton release. Our results indicate that O1 channel...
Abstract In natural photosynthesis, the light-driven splitting of water into electrons, protons and molecular oxygen forms first step solar-to-chemical energy conversion process. The reaction takes place in photosystem II, where Mn 4 CaO 5 cluster stores four oxidizing equivalents, S 0 to intermediate states Kok cycle, sequentially generated by photochemical charge separations center then catalyzes O–O bond formation chemistry 1–3 . Here, we report room temperature snapshots serial...
Photosystem II starts the photosynthetic electron transport chain that converts solar energy into chemical and thus sustains life on Earth. It catalyzes two reactions: water oxidation to molecular oxygen plastoquinone reduction. Coupling of proton transfer is crucial for efficiency; however, basis these processes remains speculative owing uncertain binding sites lack experimentally determined hydrogen positions. We collected high-resolution cryo-electron microscopy data fully hydrated...
In plants, algae and cyanobacteria, Photosystem II (PSII) catalyzes the light-driven splitting of water at a protein-bound Mn4CaO5-cluster, water-oxidizing complex (WOC). photosynthetic organisms, formation WOC from dissolved metal ions is key process because it essential in both initial activation continuous repair PSII. Structural information required for understanding this chaperone-free metal-cluster assembly. For first time, we obtained structure PSII Thermosynechococcus elongatus...
Detection of structural variants (SVs) is currently biased toward those that alter copy number. The relative contribution inversions genetic disease unclear. In this study, we analyzed genome sequencing data for 33,924 families with rare from the 100,000 Genomes Project. From a database hosting >500 million SVs, focused on 351 genes where haploinsufficiency confirmed mechanism and identified 47 ultra-rare rearrangements included an inversion (24 bp to 36.4 Mb, 20/47 de novo). Validation...
<title>Abstract</title> Photosystem II (PSII) is a large membrane-bound protein complex that essential for oxygenic photosynthesis in plants and cyanobacteria. While there are several high-resolution structures of PS from cyanobacteria, very few have been reported higher plants. Here, we present the first structure an intact active <italic>Arabidopsis thaliana</italic> at resolution 2.44 Å, which to date represents highest any land plant. The improved allowed modeling cofactors not...
The water oxidation reaction in photosystem II (PS II) produces most of the molecular oxygen atmosphere, which sustains life on Earth, and this process releases four electrons protons that drive downstream CO
Abstract Chlorophyll is essential in photosynthesis, converting sunlight into chemical energy plants, algae, and certain bacteria. Its structure, featuring a porphyrin ring enclosing central magnesium ion, varies forms like chlorophyll , b c d f allowing light absorption at broader spectrum. With 20‐carbon phytyl tail (except for ), anchored to proteins. Previous findings suggested the presence of with modified farnesyl thermophilic cyanobacteria Thermosynechoccocus vestitus . In our...
Self-resonant microhelix provides sensitivity for X-band electron paramagnetic resonance on protein crystals with <27-nl volume.
Detergents are widely used for the isolation and solubilization of membrane proteins to support crystallization structure determination. amphiphilic molecules that form micelles once characteristic critical micelle concentration (CMC) is achieved can solubilize by formation around them. The results presented a study observed in situ dynamic light-scattering (DLS) analyses performed on selected detergent solutions using newly designed advanced hardware device. DLS was initially applied...
Abstract Photosystem I (PS I) has a symmetric structure with two highly similar branches of pigments at the center that are involved in electron transfer, but shows very different efficiency along branches. We have determined cyanobacterial PS room temperature (RT) using femtosecond X-ray pulses from an free laser (XFEL) clear expansion entire protein complex direction membrane plane, when compared to previous cryogenic structures. This trend was observed by complementary datasets taken...
Albeit achieving the X-ray diffraction structure of dimeric photosystem II core complexes (dPSIIcc) at atomic resolution, nature detergent belt surrounding dPSIIcc remains ambiguous. Therefore, solution whole detergent-protein complex Thermosynechococcus elongatus (T. elongatus) solubilized in n-dodecyl-ß-d-maltoside (ßDM) was investigated by a combination small-angle scattering (SAXS) and neutron (SANS) with contrast variation. First, studied separately SANS experiments using 5% D2O....
In the last ten years, x-ray free-electron lasers (XFELs) have been successfully employed to characterize metalloproteins at room temperature using various techniques including diffraction, scattering, and spectroscopy. The approach has outrun radiation damage by femtosecond (fs) pulses. An example of an important sensitive active metal center is Mn4CaO5 cluster in photosystem II (PS II), catalytic site photosynthetic water oxidation. combination serial crystallography Kβ emission...
Photosystem II (PSII) catalyzes the photo-oxidation of water to molecular oxygen and protons. The splitting reaction occurs inside oxygen-evolving complex (OEC) via a Mn4CaO5 cluster. To elucidate mechanism, detailed structural information for each intermediate state OEC is required. Despite current high-resolution crystal structure PSII at 1.85 Å other efforts follow changes cluster using X-ray free electron laser (XFEL) crystallography in addition spectroscopic methods, many details about...
Abstract Photosystem II starts the photosynthetic electron transport chain that converts solar energy into chemical and thereby sustains life on Earth. It catalyzes two reactions, plastoquinone reduction water oxidation to molecular oxygen, which both are performed at sequestered sites. While it is known proton-coupled transfer crucial for these processes, details have remained speculative due incomplete structural data. Thus, we collected high-resolution cryo-EM data of photosystem from...