A5017455265- Metal-Catalyzed Oxygenation Mechanisms
- Enzyme Structure and Function
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Tuberculosis Research and Epidemiology
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- Porphyrin Metabolism and Disorders
- Microbial metabolism and enzyme function
- Advanced Electron Microscopy Techniques and Applications
- Atmospheric and Environmental Gas Dynamics
- Advanced Queuing Theory Analysis
- Metal complexes synthesis and properties
- Synthesis and Biological Evaluation
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- Parasitic Infections and Diagnostics
- Protein purification and stability
- Studies on Chitinases and Chitosanases
- Iron Metabolism and Disorders
- Synthesis and biological activity
- Antimicrobial Resistance in Staphylococcus
- Animal Virus Infections Studies
- Probability and Risk Models
Stockholm University
2014-2025
Rajiv Gandhi University of Health Sciences
2020-2024
GITAM University
2024
Institute for Systems Biology
2018-2022
North Seattle College
2020
Seattle University
2020
InSysBio (Russia)
2018-2019
Bangalore University
1992-2016
Indian Veterinary Research Institute
2016
Indian Institute of Technology Bombay
2015
Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme that catalyzes the conversion of to methanol at ambient temperature using nonheme, oxygen-bridged dinuclear iron cluster in active site. Structural changes hydroxylase component (sMMOH) containing diiron caused by complex formation with regulatory (MMOB) and reduction are important for regulation O
Aerobic ribonucleotide reductases (RNRs) initiate synthesis of DNA building blocks by generating a free radical within the R2 subunit; is subsequently shuttled to catalytic R1 subunit through proton-coupled electron transfer (PCET). We present high-resolution room temperature structure class Ie protein captured x-ray laser serial femtosecond crystallography. The reveals conformational reorganization shield and connect it translocation path, with structural changes propagating surface where...
Background: Joint ill is a septicemic polyarthritis condition due to the localization of pathogenic bacteria within joints young calves. The emergence antibiotic resistant pathogen in such cases may lead further complication treatment. Thus, present study was aimed isolate and identify bacterial pathogens its antimicrobial genes from joint Methods: A total 31 pus swabs were collected calves subjected for isolation identification by phenotypic genotypic method followed determination (tet mecA...
Abstract Class I ribonucleotide reductases (RNRs) convert ribonucleotides into deoxyribonucleotides under oxic conditions. The R2 subunit provides a radical required for catalysis conducted by the R1 subunit. In most R2s is generated on tyrosine via oxidation an adjacent metal site. discovery of metal-free defined new RNR subclass Ie. R2e, three otherwise strictly conserved metal-binding glutamates in active site are substituted. Two variants have been found, VPK and QSK. To date, version...
Understanding the structure of an active pharmaceutical ingredient is essential for gaining insights into its physicochemical properties. Sodium valproate, one most effective antiepileptic drugs, was first approved medical use in 1967. However, anhydrous form has remained unresolved. This because it difficult to grow crystals sufficient size single-crystal X-ray diffraction (SCXRD). Although 3D electron (3D ED) can be used studying too small SCXRD, sodium valproate are extremely sensitive...
Mycobacterium tuberculosis (MTB) displays the remarkable ability to transition in and out of dormancy, a hallmark pathogen's capacity evade immune system exploit susceptible individuals. Uncovering gene regulatory programs that underlie phenotypic shifts MTB during disease latency reactivation has posed challenge. We develop an experimental precisely control dissolved oxygen levels cultures order capture transcriptional events unfold as transitions into hypoxia-induced dormancy. Using...
Aim: The present study was conducted to isolate and characterize canine parvovirus circulating in Southern India by genetic analysis of VP2 capsid protein gene. Materials Methods:In this study, 128 samples were collected from nine different locations covering five Indian states (Pondicherry, Tamil Nadu, Kerala, Andhra Pradesh Karnataka) .Out samples, 69 found be positive PCR assay.Out 36 randomly selected processed for virus isolation.Twenty viruses could isolated successfully 18 subjected...
The present study was conducted to determine the zoonotic importance of methicillin resistant staphylococci associated with bovine mastitis and their potential role in transmission animal handlers.
Upstream open reading frames (uORFs) and upstream AUGs (uAUGs) can regulate the translation of downstream ORFs. The AT rich genome Plasmodium falciparum, due to higher content start stop codons, has potential give rise a large number uORFs uAUGs that may affect expression their flanking A bioinformatics approach was used detect uATGs associated with different genes in parasite. To study effect some these on ORF, promoters 5′ leaders containing were cloned luciferase reporter gene. Luciferase...
Maximum likelihood and uniform minimum variance unbiased estimators of steady-state probability distribution system size, at least ℓ customers in the steady state, certain measures effectiveness M/M/1 queue are obtained/derived based on observations X, number customer arrivals during a service time. The compared using Asympotic Expected Deficiency (AED) criterion leading to recommendation over maximum for some measures.
Antibody-recruiting protein-catalyzed capture agent (AR-PCCs) are a new class of all-synthetic and highly targeted antibiotics that recruit endogenous immune responses to eliminate drug-resistant microbes.
Mycobacterium tuberculosis (MTB) persists and survives antibiotic treatments by generating phenotypically heterogeneous drug-tolerant subpopulations. The surviving cells, persisters, are a major barrier to the relapse-free treatment of (TB), which is already killing >1.8 million people every year becoming deadlier with emergence multidrug-resistant strains.
Redox reactions are central to biochemistry and both controlled by induce protein structural changes. Here, we describe rearrangements crosstalk within the Bacillus cereus ribonucleotide reductase R2b-NrdI complex, a di-metal carboxylate-flavoprotein system, as part of mechanism generating essential catalytic free radical enzyme. Femtosecond crystallography at an X-ray electron laser was utilized obtain structures room temperature in defined redox states without suffering photoreduction....
In the imbedded Markov chain (IMC) analysis of M/G/1 queuing system, X1, X2, …, Xn, … form a sequence i.i.d random variables. where Xn denotes number customer arrivals during service time customer. M/D/1 queue, distribution common variable X is Poisson with mean ρ, traffic intensity. This fact utilized for maximum likelihood (ML) and uniformly minimum variance unbiased (UMVU) estimation intensity, performance measures, transition probabilities IMC, correlation functions departure process,...
There is an urgent need for strategies to discover secondary drugs prevent or disrupt antimicrobial resistance (AMR), which causing >700,000 deaths annually. Here, we demonstrate that tetracycline-resistant (Tet
Aim: The present study was conducted to determine the prevalence of antibodies against leptospira using a genus specific and serovar antigen by indirect ELISA (iELISA) Microscopic Agglutination test (MAT), respectively.Materials Methods: 300 sera samples were collected from dogs, Pondicherry, suspected be suffering Canine leptospirosis with no history vaccination.Using outer membrane proteins (OMP's) Leptospira interrogans canicola as leptospiral detected iELISA compared MAT five commonly...